Lycium barbarum polysaccharide enhances development of previously-cryopreserved murine two-cell embryos via restoration of mitochondrial function and down-regulated generation of reactive oxygen species
Lycium barbarum polysaccharide (LBP) exhibits multiple pharmacological and biological effects, including displaying antioxidant and cytoprotective properties. The current study investigated the effects of LBP-supplemented culture medium on mitochondrial distribution, mitochondrial membrane potential...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Society for Reproduction and Development
2019-01-01
|
| Series: | The Journal of Reproduction and Development |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jrd/65/2/65_2018-104/_pdf/-char/en |
| _version_ | 1797614766969061376 |
|---|---|
| author | Lei YANG Zhen GAO Lanjie LEI Qizhuang LV Qihan ZHAO Lixin LI Xiaoming CAO Wenxue FU |
| author_facet | Lei YANG Zhen GAO Lanjie LEI Qizhuang LV Qihan ZHAO Lixin LI Xiaoming CAO Wenxue FU |
| author_sort | Lei YANG |
| collection | DOAJ |
| description | Lycium barbarum polysaccharide (LBP) exhibits multiple pharmacological and biological effects, including displaying antioxidant and cytoprotective properties. The current study investigated the effects of LBP-supplemented culture medium on mitochondrial distribution, mitochondrial membrane potential (MMP), adenosine triphosphate (ATP) production, mitochondrial deoxyribonucleic acid (mtDNA) copy number, reactive oxygen species (ROS) accumulation, and development of previously-cryopreserved murine two-cell embryos. Results indicate that LBP enhances development of such embryos, and that potential mechanisms include: (1) mitochondrial function enhancement via altering mitochondrial distribution and increasing MMP, ATP production, mtDNA copy number, and expression of genes involved in mitochondrial biogenesis and energy metabolism (NAD-dependent deacetyltransferase sirtuin-1 (SIRT1) and phosphorylated adenosine monophosphate-activated protein kinase (pAMPK)); (2) down-regulation of ROS generation and enhanced expression of the antioxidant genes glutathione peroxidase 4 (GPX4) and superoxide dismutase 1 (SOD1), thereby increasing embryo oxidative stress tolerance; and (3) increased expression of B-cell lymphoma-2 (BCL2), a critical gene for cell survival and embryo development. These results demonstrate that LBP improves development of previously-cryopreserved murine two-cell embryos via restoration of mitochondrial function and down-regulated generation of ROS. |
| first_indexed | 2024-03-11T07:15:59Z |
| format | Article |
| id | doaj.art-db0944c68a954df483b05658906fd13e |
| institution | Directory Open Access Journal |
| issn | 0916-8818 1348-4400 |
| language | English |
| last_indexed | 2024-03-11T07:15:59Z |
| publishDate | 2019-01-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj.art-db0944c68a954df483b05658906fd13e2023-11-17T08:16:59ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002019-01-0165216317010.1262/jrd.2018-104jrdLycium barbarum polysaccharide enhances development of previously-cryopreserved murine two-cell embryos via restoration of mitochondrial function and down-regulated generation of reactive oxygen speciesLei YANG0Zhen GAO1Lanjie LEI2Qizhuang LV3Qihan ZHAO4Lixin LI5Xiaoming CAO6Wenxue FU7College of Basic Medical Science, Jiujiang University, Jiangxi 332000, ChinaKey Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Shaanxi 712100, ChinaAffiliated Hospital of Jiujiang University, Jiujiang University, Jiangxi 332000, ChinaCollege of Biology & Pharmacy, Yulin Normal University, Guangxi 53700, ChinaKey Laboratory of System Bio-medicine of Jiangxi Province, Jiujiang University, Jiangxi 332000, ChinaCollege of Basic Medical Science, Jiujiang University, Jiangxi 332000, ChinaCollege of Basic Medical Science, Jiujiang University, Jiangxi 332000, ChinaCollege of Basic Medical Science, Jiujiang University, Jiangxi 332000, ChinaLycium barbarum polysaccharide (LBP) exhibits multiple pharmacological and biological effects, including displaying antioxidant and cytoprotective properties. The current study investigated the effects of LBP-supplemented culture medium on mitochondrial distribution, mitochondrial membrane potential (MMP), adenosine triphosphate (ATP) production, mitochondrial deoxyribonucleic acid (mtDNA) copy number, reactive oxygen species (ROS) accumulation, and development of previously-cryopreserved murine two-cell embryos. Results indicate that LBP enhances development of such embryos, and that potential mechanisms include: (1) mitochondrial function enhancement via altering mitochondrial distribution and increasing MMP, ATP production, mtDNA copy number, and expression of genes involved in mitochondrial biogenesis and energy metabolism (NAD-dependent deacetyltransferase sirtuin-1 (SIRT1) and phosphorylated adenosine monophosphate-activated protein kinase (pAMPK)); (2) down-regulation of ROS generation and enhanced expression of the antioxidant genes glutathione peroxidase 4 (GPX4) and superoxide dismutase 1 (SOD1), thereby increasing embryo oxidative stress tolerance; and (3) increased expression of B-cell lymphoma-2 (BCL2), a critical gene for cell survival and embryo development. These results demonstrate that LBP improves development of previously-cryopreserved murine two-cell embryos via restoration of mitochondrial function and down-regulated generation of ROS.https://www.jstage.jst.go.jp/article/jrd/65/2/65_2018-104/_pdf/-char/encryopreservationlycium barbarum polysaccharidemitochondriareactive oxygen speciestwo-cell embryos |
| spellingShingle | Lei YANG Zhen GAO Lanjie LEI Qizhuang LV Qihan ZHAO Lixin LI Xiaoming CAO Wenxue FU Lycium barbarum polysaccharide enhances development of previously-cryopreserved murine two-cell embryos via restoration of mitochondrial function and down-regulated generation of reactive oxygen species The Journal of Reproduction and Development cryopreservation lycium barbarum polysaccharide mitochondria reactive oxygen species two-cell embryos |
| title | Lycium barbarum polysaccharide enhances development of previously-cryopreserved murine two-cell embryos via restoration of mitochondrial function and down-regulated generation of reactive oxygen species |
| title_full | Lycium barbarum polysaccharide enhances development of previously-cryopreserved murine two-cell embryos via restoration of mitochondrial function and down-regulated generation of reactive oxygen species |
| title_fullStr | Lycium barbarum polysaccharide enhances development of previously-cryopreserved murine two-cell embryos via restoration of mitochondrial function and down-regulated generation of reactive oxygen species |
| title_full_unstemmed | Lycium barbarum polysaccharide enhances development of previously-cryopreserved murine two-cell embryos via restoration of mitochondrial function and down-regulated generation of reactive oxygen species |
| title_short | Lycium barbarum polysaccharide enhances development of previously-cryopreserved murine two-cell embryos via restoration of mitochondrial function and down-regulated generation of reactive oxygen species |
| title_sort | lycium barbarum polysaccharide enhances development of previously cryopreserved murine two cell embryos via restoration of mitochondrial function and down regulated generation of reactive oxygen species |
| topic | cryopreservation lycium barbarum polysaccharide mitochondria reactive oxygen species two-cell embryos |
| url | https://www.jstage.jst.go.jp/article/jrd/65/2/65_2018-104/_pdf/-char/en |
| work_keys_str_mv | AT leiyang lyciumbarbarumpolysaccharideenhancesdevelopmentofpreviouslycryopreservedmurinetwocellembryosviarestorationofmitochondrialfunctionanddownregulatedgenerationofreactiveoxygenspecies AT zhengao lyciumbarbarumpolysaccharideenhancesdevelopmentofpreviouslycryopreservedmurinetwocellembryosviarestorationofmitochondrialfunctionanddownregulatedgenerationofreactiveoxygenspecies AT lanjielei lyciumbarbarumpolysaccharideenhancesdevelopmentofpreviouslycryopreservedmurinetwocellembryosviarestorationofmitochondrialfunctionanddownregulatedgenerationofreactiveoxygenspecies AT qizhuanglv lyciumbarbarumpolysaccharideenhancesdevelopmentofpreviouslycryopreservedmurinetwocellembryosviarestorationofmitochondrialfunctionanddownregulatedgenerationofreactiveoxygenspecies AT qihanzhao lyciumbarbarumpolysaccharideenhancesdevelopmentofpreviouslycryopreservedmurinetwocellembryosviarestorationofmitochondrialfunctionanddownregulatedgenerationofreactiveoxygenspecies AT lixinli lyciumbarbarumpolysaccharideenhancesdevelopmentofpreviouslycryopreservedmurinetwocellembryosviarestorationofmitochondrialfunctionanddownregulatedgenerationofreactiveoxygenspecies AT xiaomingcao lyciumbarbarumpolysaccharideenhancesdevelopmentofpreviouslycryopreservedmurinetwocellembryosviarestorationofmitochondrialfunctionanddownregulatedgenerationofreactiveoxygenspecies AT wenxuefu lyciumbarbarumpolysaccharideenhancesdevelopmentofpreviouslycryopreservedmurinetwocellembryosviarestorationofmitochondrialfunctionanddownregulatedgenerationofreactiveoxygenspecies |