The Effect of Ferrous Sulphate on the Gross and Histological Changes in the Body of Gastric Mucosa of Adult Albino Rats
Background:To observe the effects of Ferrous sulphate on the body of gastric mucosa of adult albino rats. Methods: An experimental study was carried out on 30 adult albino Wistar rats for 12 weeks.Two groups were made; A and B. They were divided further into A1, A2, B1 and B2 for qualitative paramet...
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| Format: | Article |
| Language: | English |
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Rawalpindi Medical University
2015-12-01
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| Series: | Journal of Rawalpindi Medical College |
| Subjects: | |
| Online Access: | https://www.journalrmc.com/index.php/JRMC/article/view/254 |
| _version_ | 1811280258768306176 |
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| author | Shahnila Nadir |
| author_facet | Shahnila Nadir |
| author_sort | Shahnila Nadir |
| collection | DOAJ |
| description | Background:To observe the effects of Ferrous sulphate on the body of gastric mucosa of adult albino rats.
Methods: An experimental study was carried out on 30 adult albino Wistar rats for 12 weeks.Two groups were made; A and B. They were divided further into A1, A2, B1 and B2 for qualitative parameters and statistical purposes.Group A was kept as control and divided into 2 sub groups; A1 and A2 and was given normal food and water. The experimental group B was divided into 2 sub groups B1 and B2. Ferrous sulphate was given orally for 4 and 12 weeks respectively through gavage needle for maximum efficacy in the experimental group.Albino rat’s stomach was dissected, fixed in 10% formalin and processed for light microscopy. The short and long term effects of ferrous sulphate were observed on 4th and 12th week respectively. For histological analysis H&E was used. Body of gastric mucosa of adult albino rat was considered for this study.
Results: Change in the colour of mucosa was found in 10 (100%) of cases at 12 weeks whereas only 8 (80%) cases showed changes after 4 weeks. Haemorrhage was seen scattered in 4 (40%) cases after 4 weeks whereas present in all 10 (100%) cases after 12 weeks. Ulcer was seen in 4 (40%) of cases after 12 weeks. Epithelial changes were seen in 5 (50%) cases at 4 and 8 (80%) of cases after 4 and 12 weeks respectively. Ulcerative changes were seen in 4 (40%), granulation tissue/ fibrosis was seen in 7 (70%) cases in experimental group only. There was a significant decrease in the weight of rats from 226.69 ±22.31mg to 169.43±22.39mg in the experimental group with p ˂.001. Inflammatory cell count significantly increased from (4.03± 1.02) to 17.01±5.01 in the experimental group with p˂.001.
Conclusion: Ferrous Sulphate has detrimental effects on the gastric mucosa of adult albino rats. Haemorrhages, ulcerated areas and change in colour of mucosa was observed with naked eye. Inflammatory cell infiltrate was found to be the most common presentation. |
| first_indexed | 2024-04-13T01:10:26Z |
| format | Article |
| id | doaj.art-db0975e088e34e7f8eb4e1726459b80f |
| institution | Directory Open Access Journal |
| issn | 1683-3562 1683-3570 |
| language | English |
| last_indexed | 2024-04-13T01:10:26Z |
| publishDate | 2015-12-01 |
| publisher | Rawalpindi Medical University |
| record_format | Article |
| series | Journal of Rawalpindi Medical College |
| spelling | doaj.art-db0975e088e34e7f8eb4e1726459b80f2022-12-22T03:09:10ZengRawalpindi Medical UniversityJournal of Rawalpindi Medical College1683-35621683-35702015-12-01193The Effect of Ferrous Sulphate on the Gross and Histological Changes in the Body of Gastric Mucosa of Adult Albino RatsShahnila Nadir0Department of Anatomy, Women Medical College, Abbottabad;Background:To observe the effects of Ferrous sulphate on the body of gastric mucosa of adult albino rats. Methods: An experimental study was carried out on 30 adult albino Wistar rats for 12 weeks.Two groups were made; A and B. They were divided further into A1, A2, B1 and B2 for qualitative parameters and statistical purposes.Group A was kept as control and divided into 2 sub groups; A1 and A2 and was given normal food and water. The experimental group B was divided into 2 sub groups B1 and B2. Ferrous sulphate was given orally for 4 and 12 weeks respectively through gavage needle for maximum efficacy in the experimental group.Albino rat’s stomach was dissected, fixed in 10% formalin and processed for light microscopy. The short and long term effects of ferrous sulphate were observed on 4th and 12th week respectively. For histological analysis H&E was used. Body of gastric mucosa of adult albino rat was considered for this study. Results: Change in the colour of mucosa was found in 10 (100%) of cases at 12 weeks whereas only 8 (80%) cases showed changes after 4 weeks. Haemorrhage was seen scattered in 4 (40%) cases after 4 weeks whereas present in all 10 (100%) cases after 12 weeks. Ulcer was seen in 4 (40%) of cases after 12 weeks. Epithelial changes were seen in 5 (50%) cases at 4 and 8 (80%) of cases after 4 and 12 weeks respectively. Ulcerative changes were seen in 4 (40%), granulation tissue/ fibrosis was seen in 7 (70%) cases in experimental group only. There was a significant decrease in the weight of rats from 226.69 ±22.31mg to 169.43±22.39mg in the experimental group with p ˂.001. Inflammatory cell count significantly increased from (4.03± 1.02) to 17.01±5.01 in the experimental group with p˂.001. Conclusion: Ferrous Sulphate has detrimental effects on the gastric mucosa of adult albino rats. Haemorrhages, ulcerated areas and change in colour of mucosa was observed with naked eye. Inflammatory cell infiltrate was found to be the most common presentation.https://www.journalrmc.com/index.php/JRMC/article/view/254Ferrous sulphateGastric mucosa |
| spellingShingle | Shahnila Nadir The Effect of Ferrous Sulphate on the Gross and Histological Changes in the Body of Gastric Mucosa of Adult Albino Rats Journal of Rawalpindi Medical College Ferrous sulphate Gastric mucosa |
| title | The Effect of Ferrous Sulphate on the Gross and Histological Changes in the Body of Gastric Mucosa of Adult Albino Rats |
| title_full | The Effect of Ferrous Sulphate on the Gross and Histological Changes in the Body of Gastric Mucosa of Adult Albino Rats |
| title_fullStr | The Effect of Ferrous Sulphate on the Gross and Histological Changes in the Body of Gastric Mucosa of Adult Albino Rats |
| title_full_unstemmed | The Effect of Ferrous Sulphate on the Gross and Histological Changes in the Body of Gastric Mucosa of Adult Albino Rats |
| title_short | The Effect of Ferrous Sulphate on the Gross and Histological Changes in the Body of Gastric Mucosa of Adult Albino Rats |
| title_sort | effect of ferrous sulphate on the gross and histological changes in the body of gastric mucosa of adult albino rats |
| topic | Ferrous sulphate Gastric mucosa |
| url | https://www.journalrmc.com/index.php/JRMC/article/view/254 |
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