Intraovarian Injection of Reconstituted Lyophilized Growth-Promoting Factor Extracted from Horse Blood Platelets (L-GF<sup>equina</sup>) Increases Oocytes Recovery and In Vitro Embryo Production in Holstein Cows
The purpose of this study was to determine the impact of intraovarian injections of a reconstituted lyophilized growth-promoting factor extracted from horse blood platelets (L-GF<sup>equina</sup>) on the number of ovarian follicles, the recovery of cumulus–oocyte complexes (COCs), and em...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/12/19/2618 |
| _version_ | 1827655523075358720 |
|---|---|
| author | Silviu-Ionuț Borş Dan-Lucian Dascălu Alina Borş Hossam M. Fahmy Omaima M. Kandil Ahmed Sabry S. Abdoon |
| author_facet | Silviu-Ionuț Borş Dan-Lucian Dascălu Alina Borş Hossam M. Fahmy Omaima M. Kandil Ahmed Sabry S. Abdoon |
| author_sort | Silviu-Ionuț Borş |
| collection | DOAJ |
| description | The purpose of this study was to determine the impact of intraovarian injections of a reconstituted lyophilized growth-promoting factor extracted from horse blood platelets (L-GF<sup>equina</sup>) on the number of ovarian follicles, the recovery of cumulus–oocyte complexes (COCs), and embryo development to the blastocyst stage in Holstein cows. Thus, 12 Holstein cows were assigned to three protocols. According to the number of punctured follicles in protocol 1, ovum pick-up (OPU) was conducted on days 6 and 14 of the cycle (day 0 = estrus). In protocol 2, every large follicle (more than 7 mm) was removed, and 1 mL of L-GF<sup>equina</sup> was intraovarian injected (day 0). Two days later, equine chorionic gonadotropin (eCG) was administered, and OPU sessions were conducted on days 6, 10, and 14. The same ovarian stimulation procedure as that in protocol 2 was performed in protocol 3, except that equine L-GF<sup>equina</sup> was not supplied. OPU was carried out on days 6 and 10 of the cycle. The results indicate that the intraovarian injection of L-GF<sup>equina</sup> significantly (<i>p</i> < 0.05) increased the number of OPU sessions per cycle, the recovery of cumulus–oocyte complexes (COCs), and the production of blastocysts. In conclusion, an intraovarian injection of L-GF<sup>equina</sup> can improves OPU-IVEP results in Holstein cows. |
| first_indexed | 2024-03-09T22:08:52Z |
| format | Article |
| id | doaj.art-db0bc68e87854dada00b50e31954f8f3 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T22:08:52Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-db0bc68e87854dada00b50e31954f8f32023-11-23T19:36:47ZengMDPI AGAnimals2076-26152022-09-011219261810.3390/ani12192618Intraovarian Injection of Reconstituted Lyophilized Growth-Promoting Factor Extracted from Horse Blood Platelets (L-GF<sup>equina</sup>) Increases Oocytes Recovery and In Vitro Embryo Production in Holstein CowsSilviu-Ionuț Borş0Dan-Lucian Dascălu1Alina Borş2Hossam M. Fahmy3Omaima M. Kandil4Ahmed Sabry S. Abdoon5Research and Development Station for Cattle Breeding Dancu, Holboca, 707252 Iaşi, RomaniaResearch and Development Station for Cattle Breeding Dancu, Holboca, 707252 Iaşi, RomaniaDepartment of Public Health, Faculty of Veterinary Medicine, University of Life Sciences “Ion Ionescu de la Brad” Iaşi, 700490 Iaşi, RomaniaClinical Laboratory and Blood Bank Department, Faculty of Medicine, Ain Shams University, Cairo 1181, EgyptDepartment of Animal Reproduction and Artificial Insemination, Veterinary Research Institute, National Research Centre, Dokki, Cairo 12622, EgyptDepartment of Animal Reproduction and Artificial Insemination, Veterinary Research Institute, National Research Centre, Dokki, Cairo 12622, EgyptThe purpose of this study was to determine the impact of intraovarian injections of a reconstituted lyophilized growth-promoting factor extracted from horse blood platelets (L-GF<sup>equina</sup>) on the number of ovarian follicles, the recovery of cumulus–oocyte complexes (COCs), and embryo development to the blastocyst stage in Holstein cows. Thus, 12 Holstein cows were assigned to three protocols. According to the number of punctured follicles in protocol 1, ovum pick-up (OPU) was conducted on days 6 and 14 of the cycle (day 0 = estrus). In protocol 2, every large follicle (more than 7 mm) was removed, and 1 mL of L-GF<sup>equina</sup> was intraovarian injected (day 0). Two days later, equine chorionic gonadotropin (eCG) was administered, and OPU sessions were conducted on days 6, 10, and 14. The same ovarian stimulation procedure as that in protocol 2 was performed in protocol 3, except that equine L-GF<sup>equina</sup> was not supplied. OPU was carried out on days 6 and 10 of the cycle. The results indicate that the intraovarian injection of L-GF<sup>equina</sup> significantly (<i>p</i> < 0.05) increased the number of OPU sessions per cycle, the recovery of cumulus–oocyte complexes (COCs), and the production of blastocysts. In conclusion, an intraovarian injection of L-GF<sup>equina</sup> can improves OPU-IVEP results in Holstein cows.https://www.mdpi.com/2076-2615/12/19/2618blastocysts productionL-GF<sup>equina</sup>follicular developmentHolstein cowOPU-IVEP |
| spellingShingle | Silviu-Ionuț Borş Dan-Lucian Dascălu Alina Borş Hossam M. Fahmy Omaima M. Kandil Ahmed Sabry S. Abdoon Intraovarian Injection of Reconstituted Lyophilized Growth-Promoting Factor Extracted from Horse Blood Platelets (L-GF<sup>equina</sup>) Increases Oocytes Recovery and In Vitro Embryo Production in Holstein Cows Animals blastocysts production L-GF<sup>equina</sup> follicular development Holstein cow OPU-IVEP |
| title | Intraovarian Injection of Reconstituted Lyophilized Growth-Promoting Factor Extracted from Horse Blood Platelets (L-GF<sup>equina</sup>) Increases Oocytes Recovery and In Vitro Embryo Production in Holstein Cows |
| title_full | Intraovarian Injection of Reconstituted Lyophilized Growth-Promoting Factor Extracted from Horse Blood Platelets (L-GF<sup>equina</sup>) Increases Oocytes Recovery and In Vitro Embryo Production in Holstein Cows |
| title_fullStr | Intraovarian Injection of Reconstituted Lyophilized Growth-Promoting Factor Extracted from Horse Blood Platelets (L-GF<sup>equina</sup>) Increases Oocytes Recovery and In Vitro Embryo Production in Holstein Cows |
| title_full_unstemmed | Intraovarian Injection of Reconstituted Lyophilized Growth-Promoting Factor Extracted from Horse Blood Platelets (L-GF<sup>equina</sup>) Increases Oocytes Recovery and In Vitro Embryo Production in Holstein Cows |
| title_short | Intraovarian Injection of Reconstituted Lyophilized Growth-Promoting Factor Extracted from Horse Blood Platelets (L-GF<sup>equina</sup>) Increases Oocytes Recovery and In Vitro Embryo Production in Holstein Cows |
| title_sort | intraovarian injection of reconstituted lyophilized growth promoting factor extracted from horse blood platelets l gf sup equina sup increases oocytes recovery and in vitro embryo production in holstein cows |
| topic | blastocysts production L-GF<sup>equina</sup> follicular development Holstein cow OPU-IVEP |
| url | https://www.mdpi.com/2076-2615/12/19/2618 |
| work_keys_str_mv | AT silviuionutbors intraovarianinjectionofreconstitutedlyophilizedgrowthpromotingfactorextractedfromhorsebloodplateletslgfsupequinasupincreasesoocytesrecoveryandinvitroembryoproductioninholsteincows AT danluciandascalu intraovarianinjectionofreconstitutedlyophilizedgrowthpromotingfactorextractedfromhorsebloodplateletslgfsupequinasupincreasesoocytesrecoveryandinvitroembryoproductioninholsteincows AT alinabors intraovarianinjectionofreconstitutedlyophilizedgrowthpromotingfactorextractedfromhorsebloodplateletslgfsupequinasupincreasesoocytesrecoveryandinvitroembryoproductioninholsteincows AT hossammfahmy intraovarianinjectionofreconstitutedlyophilizedgrowthpromotingfactorextractedfromhorsebloodplateletslgfsupequinasupincreasesoocytesrecoveryandinvitroembryoproductioninholsteincows AT omaimamkandil intraovarianinjectionofreconstitutedlyophilizedgrowthpromotingfactorextractedfromhorsebloodplateletslgfsupequinasupincreasesoocytesrecoveryandinvitroembryoproductioninholsteincows AT ahmedsabrysabdoon intraovarianinjectionofreconstitutedlyophilizedgrowthpromotingfactorextractedfromhorsebloodplateletslgfsupequinasupincreasesoocytesrecoveryandinvitroembryoproductioninholsteincows |