Transgenerational dynamics of rDNA copy number in Drosophila male germline stem cells
rDNA loci, composed of hundreds of tandemly duplicated arrays of rRNA genes, are known to be among the most unstable genetic elements due to their repetitive nature. rDNA instability underlies aging (replicative senescence) in yeast cells, however, its contribution to the aging of multicellular orga...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2018-02-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/32421 |
| _version_ | 1818019286439952384 |
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| author | Kevin L Lu Jonathan O Nelson George J Watase Natalie Warsinger-Pepe Yukiko M Yamashita |
| author_facet | Kevin L Lu Jonathan O Nelson George J Watase Natalie Warsinger-Pepe Yukiko M Yamashita |
| author_sort | Kevin L Lu |
| collection | DOAJ |
| description | rDNA loci, composed of hundreds of tandemly duplicated arrays of rRNA genes, are known to be among the most unstable genetic elements due to their repetitive nature. rDNA instability underlies aging (replicative senescence) in yeast cells, however, its contribution to the aging of multicellular organisms is poorly understood. In this study, we investigate the dynamics of rDNA loci during aging in the Drosophila male germline stem cell (GSC) lineage, and show that rDNA copy number decreases during aging. Our study further reveals that this age-dependent decrease in rDNA copy number is heritable from generation to generation, yet GSCs in young animals that inherited reduced rDNA copy number are capable of recovering normal rDNA copy number. Based on these findings, we propose that rDNA loci are dynamic genetic elements, where rDNA copy number changes dynamically yet is maintained through a recovery mechanism in the germline. |
| first_indexed | 2024-04-14T07:49:52Z |
| format | Article |
| id | doaj.art-db0c7c713aa144559b0257da01a8f993 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:49:52Z |
| publishDate | 2018-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-db0c7c713aa144559b0257da01a8f9932022-12-22T02:05:13ZengeLife Sciences Publications LtdeLife2050-084X2018-02-01710.7554/eLife.32421Transgenerational dynamics of rDNA copy number in Drosophila male germline stem cellsKevin L Lu0https://orcid.org/0000-0003-2677-9537Jonathan O Nelson1https://orcid.org/0000-0001-9831-745XGeorge J Watase2https://orcid.org/0000-0001-8250-9027Natalie Warsinger-Pepe3https://orcid.org/0000-0002-9375-8990Yukiko M Yamashita4https://orcid.org/0000-0001-5541-0216Life Sciences Institute, University of Michigan, Ann Arbor, United States; Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, United States; Medical Scientist Training Program, University of Michigan, Ann Arbor, United StatesLife Sciences Institute, University of Michigan, Ann Arbor, United States; Howard Hughes Medical Institute, University of Michigan, Ann Arbor, United StatesLife Sciences Institute, University of Michigan, Ann Arbor, United States; Howard Hughes Medical Institute, University of Michigan, Ann Arbor, United StatesLife Sciences Institute, University of Michigan, Ann Arbor, United States; Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, United StatesLife Sciences Institute, University of Michigan, Ann Arbor, United States; Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, United States; Howard Hughes Medical Institute, University of Michigan, Ann Arbor, United States; Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, United StatesrDNA loci, composed of hundreds of tandemly duplicated arrays of rRNA genes, are known to be among the most unstable genetic elements due to their repetitive nature. rDNA instability underlies aging (replicative senescence) in yeast cells, however, its contribution to the aging of multicellular organisms is poorly understood. In this study, we investigate the dynamics of rDNA loci during aging in the Drosophila male germline stem cell (GSC) lineage, and show that rDNA copy number decreases during aging. Our study further reveals that this age-dependent decrease in rDNA copy number is heritable from generation to generation, yet GSCs in young animals that inherited reduced rDNA copy number are capable of recovering normal rDNA copy number. Based on these findings, we propose that rDNA loci are dynamic genetic elements, where rDNA copy number changes dynamically yet is maintained through a recovery mechanism in the germline.https://elifesciences.org/articles/32421rDNAagingstem cellsgermlinetransgenerational inheritance |
| spellingShingle | Kevin L Lu Jonathan O Nelson George J Watase Natalie Warsinger-Pepe Yukiko M Yamashita Transgenerational dynamics of rDNA copy number in Drosophila male germline stem cells eLife rDNA aging stem cells germline transgenerational inheritance |
| title | Transgenerational dynamics of rDNA copy number in Drosophila male germline stem cells |
| title_full | Transgenerational dynamics of rDNA copy number in Drosophila male germline stem cells |
| title_fullStr | Transgenerational dynamics of rDNA copy number in Drosophila male germline stem cells |
| title_full_unstemmed | Transgenerational dynamics of rDNA copy number in Drosophila male germline stem cells |
| title_short | Transgenerational dynamics of rDNA copy number in Drosophila male germline stem cells |
| title_sort | transgenerational dynamics of rdna copy number in drosophila male germline stem cells |
| topic | rDNA aging stem cells germline transgenerational inheritance |
| url | https://elifesciences.org/articles/32421 |
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