Outrunning damage: Electrons vs X-rays—timescales and mechanisms
Toward the end of his career, Zewail developed strong interest in fast electron spectroscopy and imaging, a field to which he made important contributions toward his aim of making molecular movies free of radiation damage. We therefore compare here the atomistic mechanisms leading to destruction of...
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| Format: | Article |
| Language: | English |
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AIP Publishing LLC and ACA
2017-07-01
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| Series: | Structural Dynamics |
| Online Access: | http://dx.doi.org/10.1063/1.4984606 |
| _version_ | 1811332912566501376 |
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| author | John C. H. Spence |
| author_facet | John C. H. Spence |
| author_sort | John C. H. Spence |
| collection | DOAJ |
| description | Toward the end of his career, Zewail developed strong interest in fast electron spectroscopy and imaging, a field to which he made important contributions toward his aim of making molecular movies free of radiation damage. We therefore compare here the atomistic mechanisms leading to destruction of protein samples in diffract-and-destroy experiments for the cases of high-energy electron beam irradiation and X-ray laser pulses. The damage processes and their time-scales are compared and relevant elastic, inelastic, and photoelectron cross sections are given. Inelastic mean-free paths for ejected electrons at very low energies in insulators are compared with the bioparticle size. The dose rate and structural damage rate for electrons are found to be much lower, allowing longer pulses, reduced beam current, and Coulomb interactions for the formation of smaller probes. High-angle electron scattering from the nucleus, which has no parallel in the X-ray case, tracks the slowly moving nuclei during the explosion, just as the gain of the XFEL (X-ray free-electron laser) has no parallel in the electron case. Despite reduced damage and much larger elastic scattering cross sections in the electron case, leading to not dissimilar elastic scattering rates (when account is taken of the greatly increased incident XFEL fluence), progress for single-particle electron diffraction is seen to depend on the effort to reduce emittance growth due to Coulomb interactions, and so allow formation of intense sub-micron beams no larger than a virus. |
| first_indexed | 2024-04-13T16:44:11Z |
| format | Article |
| id | doaj.art-db1036890c754fa18adebd3d5c668708 |
| institution | Directory Open Access Journal |
| issn | 2329-7778 |
| language | English |
| last_indexed | 2024-04-13T16:44:11Z |
| publishDate | 2017-07-01 |
| publisher | AIP Publishing LLC and ACA |
| record_format | Article |
| series | Structural Dynamics |
| spelling | doaj.art-db1036890c754fa18adebd3d5c6687082022-12-22T02:39:09ZengAIP Publishing LLC and ACAStructural Dynamics2329-77782017-07-0144044027044027-1210.1063/1.4984606026795SDYOutrunning damage: Electrons vs X-rays—timescales and mechanismsJohn C. H. Spence0Department of Physics, Arizona State University, Tempe, Arizona 85282, USAToward the end of his career, Zewail developed strong interest in fast electron spectroscopy and imaging, a field to which he made important contributions toward his aim of making molecular movies free of radiation damage. We therefore compare here the atomistic mechanisms leading to destruction of protein samples in diffract-and-destroy experiments for the cases of high-energy electron beam irradiation and X-ray laser pulses. The damage processes and their time-scales are compared and relevant elastic, inelastic, and photoelectron cross sections are given. Inelastic mean-free paths for ejected electrons at very low energies in insulators are compared with the bioparticle size. The dose rate and structural damage rate for electrons are found to be much lower, allowing longer pulses, reduced beam current, and Coulomb interactions for the formation of smaller probes. High-angle electron scattering from the nucleus, which has no parallel in the X-ray case, tracks the slowly moving nuclei during the explosion, just as the gain of the XFEL (X-ray free-electron laser) has no parallel in the electron case. Despite reduced damage and much larger elastic scattering cross sections in the electron case, leading to not dissimilar elastic scattering rates (when account is taken of the greatly increased incident XFEL fluence), progress for single-particle electron diffraction is seen to depend on the effort to reduce emittance growth due to Coulomb interactions, and so allow formation of intense sub-micron beams no larger than a virus.http://dx.doi.org/10.1063/1.4984606 |
| spellingShingle | John C. H. Spence Outrunning damage: Electrons vs X-rays—timescales and mechanisms Structural Dynamics |
| title | Outrunning damage: Electrons vs X-rays—timescales and mechanisms |
| title_full | Outrunning damage: Electrons vs X-rays—timescales and mechanisms |
| title_fullStr | Outrunning damage: Electrons vs X-rays—timescales and mechanisms |
| title_full_unstemmed | Outrunning damage: Electrons vs X-rays—timescales and mechanisms |
| title_short | Outrunning damage: Electrons vs X-rays—timescales and mechanisms |
| title_sort | outrunning damage electrons vs x rays timescales and mechanisms |
| url | http://dx.doi.org/10.1063/1.4984606 |
| work_keys_str_mv | AT johnchspence outrunningdamageelectronsvsxraystimescalesandmechanisms |