Mitotic activity patterns and cytoskeletal changes throughout the progression of diapause developmental program in Daphnia
Abstract Background Diapause is a form of dormancy that is genetically predetermined to allow animals to overcome harsh environmental conditions. It is induced by predictive environmental cues bringing cellular activity levels into a state of suspended animation. Entering diapause requires organisma...
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| Format: | Article |
| Language: | English |
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BMC
2018-12-01
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| Series: | BMC Cell Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12860-018-0181-0 |
| _version_ | 1828861645286277120 |
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| author | Luxi Chen Rosemary E. Barnett Martin Horstmann Verena Bamberger Lea Heberle Nina Krebs John K. Colbourne Rocío Gómez Linda C. Weiss |
| author_facet | Luxi Chen Rosemary E. Barnett Martin Horstmann Verena Bamberger Lea Heberle Nina Krebs John K. Colbourne Rocío Gómez Linda C. Weiss |
| author_sort | Luxi Chen |
| collection | DOAJ |
| description | Abstract Background Diapause is a form of dormancy that is genetically predetermined to allow animals to overcome harsh environmental conditions. It is induced by predictive environmental cues bringing cellular activity levels into a state of suspended animation. Entering diapause requires organismal, molecular and cellular adaptation to severely reduced energy flows. Cells must therefore have evolved strategies that prepare them for periods with limited metabolic resources. However, changes that occur on the (sub-)cellular level have not been thoroughly described. Results We investigated mitotic activity and we monitored cytoskeletal network changes in successive stages of diapausing and non-diapausing Daphnia magna embryos using (immuno-)fluorescent labeling. We find that embryos destined to diapause show a delayed and 2.5x slower mitotic activity in comparison to continuously developing embryos. Development is halted when D. magna embryos reach ~ 3500 cells, whereupon mitotic activity is absent and cytoskeletal components are severely reduced, rendering diapause cells compact and condensed. Conclusion In the initiation phase of diapause, the slower cell division rate points to prolonged interphase duration, preparing the cells for diapause maintenance. During diapause, cytoskeletal depletion and cellular condensation may be a means to save energy resources. Our data provide insights into the sub-cellular change of diapause in Daphnia. |
| first_indexed | 2024-12-13T03:05:56Z |
| format | Article |
| id | doaj.art-db10ea8abaa7476f986da683269e9c2b |
| institution | Directory Open Access Journal |
| issn | 1471-2121 |
| language | English |
| last_indexed | 2024-12-13T03:05:56Z |
| publishDate | 2018-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cell Biology |
| spelling | doaj.art-db10ea8abaa7476f986da683269e9c2b2022-12-22T00:01:43ZengBMCBMC Cell Biology1471-21212018-12-0119111210.1186/s12860-018-0181-0Mitotic activity patterns and cytoskeletal changes throughout the progression of diapause developmental program in DaphniaLuxi Chen0Rosemary E. Barnett1Martin Horstmann2Verena Bamberger3Lea Heberle4Nina Krebs5John K. Colbourne6Rocío Gómez7Linda C. Weiss8Department of Animal Ecology, Evolution and Biodiversity, Ruhr-University BochumSchool of Biosciences, University of BirminghamDepartment of Animal Ecology, Evolution and Biodiversity, Ruhr-University BochumDepartment of Animal Ecology, Evolution and Biodiversity, Ruhr-University BochumDepartment of Animal Ecology, Evolution and Biodiversity, Ruhr-University BochumDepartment of Animal Ecology, Evolution and Biodiversity, Ruhr-University BochumSchool of Biosciences, University of BirminghamDepartamento de Biología. Facultad de Ciencias, Universidad Autónoma de MadridDepartment of Animal Ecology, Evolution and Biodiversity, Ruhr-University BochumAbstract Background Diapause is a form of dormancy that is genetically predetermined to allow animals to overcome harsh environmental conditions. It is induced by predictive environmental cues bringing cellular activity levels into a state of suspended animation. Entering diapause requires organismal, molecular and cellular adaptation to severely reduced energy flows. Cells must therefore have evolved strategies that prepare them for periods with limited metabolic resources. However, changes that occur on the (sub-)cellular level have not been thoroughly described. Results We investigated mitotic activity and we monitored cytoskeletal network changes in successive stages of diapausing and non-diapausing Daphnia magna embryos using (immuno-)fluorescent labeling. We find that embryos destined to diapause show a delayed and 2.5x slower mitotic activity in comparison to continuously developing embryos. Development is halted when D. magna embryos reach ~ 3500 cells, whereupon mitotic activity is absent and cytoskeletal components are severely reduced, rendering diapause cells compact and condensed. Conclusion In the initiation phase of diapause, the slower cell division rate points to prolonged interphase duration, preparing the cells for diapause maintenance. During diapause, cytoskeletal depletion and cellular condensation may be a means to save energy resources. Our data provide insights into the sub-cellular change of diapause in Daphnia.http://link.springer.com/article/10.1186/s12860-018-0181-0DormancyDiapauseDaphniaCytoskeletonActinTubulin |
| spellingShingle | Luxi Chen Rosemary E. Barnett Martin Horstmann Verena Bamberger Lea Heberle Nina Krebs John K. Colbourne Rocío Gómez Linda C. Weiss Mitotic activity patterns and cytoskeletal changes throughout the progression of diapause developmental program in Daphnia BMC Cell Biology Dormancy Diapause Daphnia Cytoskeleton Actin Tubulin |
| title | Mitotic activity patterns and cytoskeletal changes throughout the progression of diapause developmental program in Daphnia |
| title_full | Mitotic activity patterns and cytoskeletal changes throughout the progression of diapause developmental program in Daphnia |
| title_fullStr | Mitotic activity patterns and cytoskeletal changes throughout the progression of diapause developmental program in Daphnia |
| title_full_unstemmed | Mitotic activity patterns and cytoskeletal changes throughout the progression of diapause developmental program in Daphnia |
| title_short | Mitotic activity patterns and cytoskeletal changes throughout the progression of diapause developmental program in Daphnia |
| title_sort | mitotic activity patterns and cytoskeletal changes throughout the progression of diapause developmental program in daphnia |
| topic | Dormancy Diapause Daphnia Cytoskeleton Actin Tubulin |
| url | http://link.springer.com/article/10.1186/s12860-018-0181-0 |
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