Comparing the Effect of Microinjection of Angiotensin II and Captopril to the Locus Coeruleus on Morphine Withdrawal Signs in Rat
Background: Locus coeruleus is one of important involved sites in morphine withdrawal signs, contains high densities of Angiotensin II receptor binding sites. In this study the effect of microinjection of Angiotensin II and captopril to the Locus coeruleus on morphine withdrawal signs was evaluated...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | fas |
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Isfahan University of Medical Sciences
2009-10-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/286 |
| _version_ | 1797719884517343232 |
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| author | Zohreh Azizolahi Hojatollah Alaei Mahmoud Hosseini Ali Asghar Pilehvarian |
| author_facet | Zohreh Azizolahi Hojatollah Alaei Mahmoud Hosseini Ali Asghar Pilehvarian |
| author_sort | Zohreh Azizolahi |
| collection | DOAJ |
| description | Background:
Locus coeruleus is one of important involved sites in morphine withdrawal signs, contains high densities of Angiotensin II receptor binding sites. In this study the effect of microinjection of Angiotensin II and captopril to the Locus coeruleus on morphine withdrawal signs was evaluated.
Methods:
Male wistar rats were anesthetized and stainless style cannula implanted in the locus coeruleus and allowed to recover from surgery. Morphine was injected 3 times in each day for 4 days to induce morphine dependence. The animals divided into 3 groups; control group received saline and case groups received Angiotensin II (1 nanomole) or captopril (30 µg) before naloxone injection. After naloxone injection, morphine withdrawal signs were assessed and compared between groups.
Findings:
In Angiotensin group, some of withdrawal signs such as wet dog shakes was significantly higher than saline group (P < 0.05). Expression of some of withdrawal signs such as jumping in Angiotensin group was decreased more than saline group (P < 0.01). Also, in captopril group some of withdrawal signs such as wet dog shakes was significantly higher than those of saline group (P < 0.05. Expression of some of withdrawal signs such as writhing posture, teeth chattering, grooming, genital grooming and head shakes were decreased in captopril group more than saline group (P < 0.05).
Conclusion:
We suggest that Angiotensin II have some roles in opioid withdrawal through locus coeruleus nucleous which contains considerable receptors for it.
Key words: Angiotensin II, Morphine, Captopril, Withdrawal, Locus coeruleus, Rat. |
| first_indexed | 2024-03-12T09:12:17Z |
| format | Article |
| id | doaj.art-db13e751bdb04e4ebb317400f88be10b |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T09:12:17Z |
| publishDate | 2009-10-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-db13e751bdb04e4ebb317400f88be10b2023-09-02T14:57:55ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2009-10-012798450459278Comparing the Effect of Microinjection of Angiotensin II and Captopril to the Locus Coeruleus on Morphine Withdrawal Signs in RatZohreh Azizolahi0Hojatollah Alaei1Mahmoud Hosseini2Ali Asghar Pilehvarian3MSc, Isfahan Payam-e-Noor University, IsfahanProfessor of Physiology, Department of Physiology, School of Medicine, Isfahan University of Medical Siences, IsfahanAssistant Professor of Physiology, Department of Physiology, School of Medicine, Mashhad University of Medical Siences, MashhadAssistant Professor of Biology, Isfahan Payam-e-Noor University, IsfahanBackground: Locus coeruleus is one of important involved sites in morphine withdrawal signs, contains high densities of Angiotensin II receptor binding sites. In this study the effect of microinjection of Angiotensin II and captopril to the Locus coeruleus on morphine withdrawal signs was evaluated. Methods: Male wistar rats were anesthetized and stainless style cannula implanted in the locus coeruleus and allowed to recover from surgery. Morphine was injected 3 times in each day for 4 days to induce morphine dependence. The animals divided into 3 groups; control group received saline and case groups received Angiotensin II (1 nanomole) or captopril (30 µg) before naloxone injection. After naloxone injection, morphine withdrawal signs were assessed and compared between groups. Findings: In Angiotensin group, some of withdrawal signs such as wet dog shakes was significantly higher than saline group (P < 0.05). Expression of some of withdrawal signs such as jumping in Angiotensin group was decreased more than saline group (P < 0.01). Also, in captopril group some of withdrawal signs such as wet dog shakes was significantly higher than those of saline group (P < 0.05. Expression of some of withdrawal signs such as writhing posture, teeth chattering, grooming, genital grooming and head shakes were decreased in captopril group more than saline group (P < 0.05). Conclusion: We suggest that Angiotensin II have some roles in opioid withdrawal through locus coeruleus nucleous which contains considerable receptors for it. Key words: Angiotensin II, Morphine, Captopril, Withdrawal, Locus coeruleus, Rat.http://jims.mui.ac.ir/index.php/jims/article/view/286 |
| spellingShingle | Zohreh Azizolahi Hojatollah Alaei Mahmoud Hosseini Ali Asghar Pilehvarian Comparing the Effect of Microinjection of Angiotensin II and Captopril to the Locus Coeruleus on Morphine Withdrawal Signs in Rat مجله دانشکده پزشکی اصفهان |
| title | Comparing the Effect of Microinjection of Angiotensin II and Captopril to the Locus Coeruleus on Morphine Withdrawal Signs in Rat |
| title_full | Comparing the Effect of Microinjection of Angiotensin II and Captopril to the Locus Coeruleus on Morphine Withdrawal Signs in Rat |
| title_fullStr | Comparing the Effect of Microinjection of Angiotensin II and Captopril to the Locus Coeruleus on Morphine Withdrawal Signs in Rat |
| title_full_unstemmed | Comparing the Effect of Microinjection of Angiotensin II and Captopril to the Locus Coeruleus on Morphine Withdrawal Signs in Rat |
| title_short | Comparing the Effect of Microinjection of Angiotensin II and Captopril to the Locus Coeruleus on Morphine Withdrawal Signs in Rat |
| title_sort | comparing the effect of microinjection of angiotensin ii and captopril to the locus coeruleus on morphine withdrawal signs in rat |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/286 |
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