Fucoidan (<i>Undaria pinnatifida</i>)/Polydopamine Composite-Modified Surface Promotes Osteogenic Potential of Periodontal Ligament Stem Cells
Fucoidan, a marine-sulfated polysaccharide derived from brown algae, has been recently spotlighted as a natural biomaterial for use in bone formation and regeneration. Current research explores the osteoinductive and osteoconductive properties of fucoidan-based composites for bone tissue engineering...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/20/3/181 |
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| author | Kyu Hwan Kwack Ju Young Ji Borami Park Jung Sun Heo |
| author_facet | Kyu Hwan Kwack Ju Young Ji Borami Park Jung Sun Heo |
| author_sort | Kyu Hwan Kwack |
| collection | DOAJ |
| description | Fucoidan, a marine-sulfated polysaccharide derived from brown algae, has been recently spotlighted as a natural biomaterial for use in bone formation and regeneration. Current research explores the osteoinductive and osteoconductive properties of fucoidan-based composites for bone tissue engineering applications. The utility of fucoidan in a bone tissue regeneration environment necessitates a better understanding of how fucoidan regulates osteogenic processes at the molecular level. Therefore, this study designed a fucoidan and polydopamine (PDA) composite-based film for use in a culture platform for periodontal ligament stem cells (PDLSCs) and explored the prominent molecular pathways induced during osteogenic differentiation of PDLSCs through transcriptome profiling. Characterization of the fucoidan/PDA-coated culture polystyrene surface was assessed by scanning electron microscopy and X-ray photoelectron spectroscopy. The osteogenic differentiation of the PDLSCs cultured on the fucoidan/PDA composite was examined through alkaline phosphatase activity, intracellular calcium levels, matrix mineralization assay, and analysis of the mRNA and protein expression of osteogenic markers. RNA sequencing was performed to identify significantly enriched and associated molecular networks. The culture of PDLSCs on the fucoidan/PDA composite demonstrated higher osteogenic potency than that on the control surface. Differentially expressed genes (DEGs) (<i>n</i> = 348) were identified during fucoidan/PDA-induced osteogenic differentiation by RNA sequencing. The signaling pathways enriched in the DEGs include regulation of the actin cytoskeleton and Ras-related protein 1 and phosphatidylinositol signaling. These pathways represent cell adhesion and cytoskeleton organization functions that are significantly involved in the osteogenic process. These results suggest that a fucoidan/PDA composite promotes the osteogenic potential of PDLSCs by activation of critical molecular pathways. |
| first_indexed | 2024-03-09T13:31:37Z |
| format | Article |
| id | doaj.art-db14e762820842609f58582c1ba41f7f |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T13:31:37Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-db14e762820842609f58582c1ba41f7f2023-11-30T21:17:57ZengMDPI AGMarine Drugs1660-33972022-02-0120318110.3390/md20030181Fucoidan (<i>Undaria pinnatifida</i>)/Polydopamine Composite-Modified Surface Promotes Osteogenic Potential of Periodontal Ligament Stem CellsKyu Hwan Kwack0Ju Young Ji1Borami Park2Jung Sun Heo3Department of Oral Biology, School of Dental Medicine, University at Buffalo, State University of New York, New York, NY 14214, USADepartment of Maxillofacial Biomedical Engineering, Institute of Oral Biology, School of Dentistry, Kyung Hee University, 26 Kyunghee-daero, Dongdaemun-gu, Seoul 02447, KoreaDepartment of Maxillofacial Biomedical Engineering, Institute of Oral Biology, School of Dentistry, Kyung Hee University, 26 Kyunghee-daero, Dongdaemun-gu, Seoul 02447, KoreaDepartment of Maxillofacial Biomedical Engineering, Institute of Oral Biology, School of Dentistry, Kyung Hee University, 26 Kyunghee-daero, Dongdaemun-gu, Seoul 02447, KoreaFucoidan, a marine-sulfated polysaccharide derived from brown algae, has been recently spotlighted as a natural biomaterial for use in bone formation and regeneration. Current research explores the osteoinductive and osteoconductive properties of fucoidan-based composites for bone tissue engineering applications. The utility of fucoidan in a bone tissue regeneration environment necessitates a better understanding of how fucoidan regulates osteogenic processes at the molecular level. Therefore, this study designed a fucoidan and polydopamine (PDA) composite-based film for use in a culture platform for periodontal ligament stem cells (PDLSCs) and explored the prominent molecular pathways induced during osteogenic differentiation of PDLSCs through transcriptome profiling. Characterization of the fucoidan/PDA-coated culture polystyrene surface was assessed by scanning electron microscopy and X-ray photoelectron spectroscopy. The osteogenic differentiation of the PDLSCs cultured on the fucoidan/PDA composite was examined through alkaline phosphatase activity, intracellular calcium levels, matrix mineralization assay, and analysis of the mRNA and protein expression of osteogenic markers. RNA sequencing was performed to identify significantly enriched and associated molecular networks. The culture of PDLSCs on the fucoidan/PDA composite demonstrated higher osteogenic potency than that on the control surface. Differentially expressed genes (DEGs) (<i>n</i> = 348) were identified during fucoidan/PDA-induced osteogenic differentiation by RNA sequencing. The signaling pathways enriched in the DEGs include regulation of the actin cytoskeleton and Ras-related protein 1 and phosphatidylinositol signaling. These pathways represent cell adhesion and cytoskeleton organization functions that are significantly involved in the osteogenic process. These results suggest that a fucoidan/PDA composite promotes the osteogenic potential of PDLSCs by activation of critical molecular pathways.https://www.mdpi.com/1660-3397/20/3/181fucoidanpolydopamineperiodontal ligament stem cellsosteogenic differentiationtranscriptome profiling |
| spellingShingle | Kyu Hwan Kwack Ju Young Ji Borami Park Jung Sun Heo Fucoidan (<i>Undaria pinnatifida</i>)/Polydopamine Composite-Modified Surface Promotes Osteogenic Potential of Periodontal Ligament Stem Cells Marine Drugs fucoidan polydopamine periodontal ligament stem cells osteogenic differentiation transcriptome profiling |
| title | Fucoidan (<i>Undaria pinnatifida</i>)/Polydopamine Composite-Modified Surface Promotes Osteogenic Potential of Periodontal Ligament Stem Cells |
| title_full | Fucoidan (<i>Undaria pinnatifida</i>)/Polydopamine Composite-Modified Surface Promotes Osteogenic Potential of Periodontal Ligament Stem Cells |
| title_fullStr | Fucoidan (<i>Undaria pinnatifida</i>)/Polydopamine Composite-Modified Surface Promotes Osteogenic Potential of Periodontal Ligament Stem Cells |
| title_full_unstemmed | Fucoidan (<i>Undaria pinnatifida</i>)/Polydopamine Composite-Modified Surface Promotes Osteogenic Potential of Periodontal Ligament Stem Cells |
| title_short | Fucoidan (<i>Undaria pinnatifida</i>)/Polydopamine Composite-Modified Surface Promotes Osteogenic Potential of Periodontal Ligament Stem Cells |
| title_sort | fucoidan i undaria pinnatifida i polydopamine composite modified surface promotes osteogenic potential of periodontal ligament stem cells |
| topic | fucoidan polydopamine periodontal ligament stem cells osteogenic differentiation transcriptome profiling |
| url | https://www.mdpi.com/1660-3397/20/3/181 |
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