Development of SSR markers for Astragalus lehmannianus, a vulnerable species from northwestern China
Premise Astragalus lehmannianus (Fabaceae) is a vulnerable species found in the cold deserts of northwestern China. We aimed to characterize polymorphic microsatellite loci for A. lehmannianus to support future studies of population genetic dynamics and conservation management of the species. Method...
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| Format: | Article |
| Language: | English |
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Wiley
2019-10-01
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| Series: | Applications in Plant Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/aps3.11297 |
| _version_ | 1811205191998898176 |
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| author | Mingying Wu Xiaojun Shi Dunyan Tan |
| author_facet | Mingying Wu Xiaojun Shi Dunyan Tan |
| author_sort | Mingying Wu |
| collection | DOAJ |
| description | Premise Astragalus lehmannianus (Fabaceae) is a vulnerable species found in the cold deserts of northwestern China. We aimed to characterize polymorphic microsatellite loci for A. lehmannianus to support future studies of population genetic dynamics and conservation management of the species. Methods and Results We used next‐generation sequencing to detect polymorphic microsatellites. Twenty‐five potential microsatellite loci were identified, 12 of which were polymorphic and present in the three study populations of A. lehmannianus. Levels of observed and expected heterozygosities were 0.000–1.000 and 0.000–0.827, respectively. Furthermore, two and five of the 12 developed primers were successfully amplified in two congeneric species, A. arpilobus and A. oxyglottis, respectively. Conclusions These newly developed microsatellite markers can be used to determine population diversity and to develop conservation strategies in A. lehmannianus. |
| first_indexed | 2024-04-12T03:27:43Z |
| format | Article |
| id | doaj.art-db1545cc8a124028afa38758c1aaae14 |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-04-12T03:27:43Z |
| publishDate | 2019-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-db1545cc8a124028afa38758c1aaae142022-12-22T03:49:40ZengWileyApplications in Plant Sciences2168-04502019-10-01710n/an/a10.1002/aps3.11297Development of SSR markers for Astragalus lehmannianus, a vulnerable species from northwestern ChinaMingying Wu0Xiaojun Shi1Dunyan Tan2Xinjiang Key Laboratory of Grassland Resources and Ecology and Ministry of Education Key Laboratory for Western Arid Region Grassland Resources and Ecology College of Grassland and Environment Sciences Xinjiang Agricultural University Ürümqi 830052 People's Republic of ChinaXinjiang Key Laboratory of Grassland Resources and Ecology and Ministry of Education Key Laboratory for Western Arid Region Grassland Resources and Ecology College of Grassland and Environment Sciences Xinjiang Agricultural University Ürümqi 830052 People's Republic of ChinaXinjiang Key Laboratory of Grassland Resources and Ecology and Ministry of Education Key Laboratory for Western Arid Region Grassland Resources and Ecology College of Grassland and Environment Sciences Xinjiang Agricultural University Ürümqi 830052 People's Republic of ChinaPremise Astragalus lehmannianus (Fabaceae) is a vulnerable species found in the cold deserts of northwestern China. We aimed to characterize polymorphic microsatellite loci for A. lehmannianus to support future studies of population genetic dynamics and conservation management of the species. Methods and Results We used next‐generation sequencing to detect polymorphic microsatellites. Twenty‐five potential microsatellite loci were identified, 12 of which were polymorphic and present in the three study populations of A. lehmannianus. Levels of observed and expected heterozygosities were 0.000–1.000 and 0.000–0.827, respectively. Furthermore, two and five of the 12 developed primers were successfully amplified in two congeneric species, A. arpilobus and A. oxyglottis, respectively. Conclusions These newly developed microsatellite markers can be used to determine population diversity and to develop conservation strategies in A. lehmannianus.https://doi.org/10.1002/aps3.11297Astragalus lehmannianusconservationFabacaeaemicrosatellite markervulnerable species |
| spellingShingle | Mingying Wu Xiaojun Shi Dunyan Tan Development of SSR markers for Astragalus lehmannianus, a vulnerable species from northwestern China Applications in Plant Sciences Astragalus lehmannianus conservation Fabacaeae microsatellite marker vulnerable species |
| title | Development of SSR markers for Astragalus lehmannianus, a vulnerable species from northwestern China |
| title_full | Development of SSR markers for Astragalus lehmannianus, a vulnerable species from northwestern China |
| title_fullStr | Development of SSR markers for Astragalus lehmannianus, a vulnerable species from northwestern China |
| title_full_unstemmed | Development of SSR markers for Astragalus lehmannianus, a vulnerable species from northwestern China |
| title_short | Development of SSR markers for Astragalus lehmannianus, a vulnerable species from northwestern China |
| title_sort | development of ssr markers for astragalus lehmannianus a vulnerable species from northwestern china |
| topic | Astragalus lehmannianus conservation Fabacaeae microsatellite marker vulnerable species |
| url | https://doi.org/10.1002/aps3.11297 |
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