Fructan mixtures of Agave salmiana and chicory exhibit in vitro anticancer potential in human colon cells and prebiotic activity
AbstractAnticancer potential and a prebiotic effect of fructan mixtures from Agave salmiana (branched) and chicory inulin (linear) were evaluated to different mass fractions 1/0, 0/1, 0.5/0.5, 0.75/0.25, 0.25/0.75, respectively, in three concentrations: 2, 3.5, and 5 mg/mL. The viability, proliferat...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
|
| Series: | Cogent Food & Agriculture |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/23311932.2023.2196871 |
| _version_ | 1797435552964804608 |
|---|---|
| author | Paola Alvarez García Luz Eugenia Alcántara Quintana Miguel Ángel Ruiz Cabrera Fidel Martínez Gutiérrez Raúl González García Alicia Grajales Lagunes |
| author_facet | Paola Alvarez García Luz Eugenia Alcántara Quintana Miguel Ángel Ruiz Cabrera Fidel Martínez Gutiérrez Raúl González García Alicia Grajales Lagunes |
| author_sort | Paola Alvarez García |
| collection | DOAJ |
| description | AbstractAnticancer potential and a prebiotic effect of fructan mixtures from Agave salmiana (branched) and chicory inulin (linear) were evaluated to different mass fractions 1/0, 0/1, 0.5/0.5, 0.75/0.25, 0.25/0.75, respectively, in three concentrations: 2, 3.5, and 5 mg/mL. The viability, proliferation, and apoptosis of human colon cell lines, CRL1831 (healthy), HT29 (cancerous grade 1–2), and SW480 (cancerous grade 3–4), were evaluated by absorbance assays. Lactobacillus acidophilus and Bifidobacterium longum subsp. infantis growth was determined by spectrophotometry at 600 nm. The lactic acid and short-chain fatty acids (SCFAs) production was determined by the HPLC method. Fructan mixtures (inulin/agave, 0.25/0.75) showed a synergic effect on viability, proliferation, and decreased apoptosis in healthy colon cells (CRL1831) at 5 mg/mL. An increase (p < 0.05) of apoptosis in HT29 and SW480 was observed with inulin/agave (0/1) at 3.5 mg/mL and inulin/agave (0.5/0.5), respectively. The proliferation of L. acidophilus and B. longum subsp. infantis was better when the agave fructans were in a higher proportion than inulin. Lactic acid was the highest metabolite produced by L. acidophilus and acetic acid by B. longum subsp. infantis. Fructan mixtures induced SW480 and HT29 cells to apoptosis without toxic effects on healthy cells and improved bacteria proliferation and metabolite production. |
| first_indexed | 2024-03-09T10:49:24Z |
| format | Article |
| id | doaj.art-db19a1e9303547e5a3ea5d4221ec5634 |
| institution | Directory Open Access Journal |
| issn | 2331-1932 |
| language | English |
| last_indexed | 2024-03-09T10:49:24Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cogent Food & Agriculture |
| spelling | doaj.art-db19a1e9303547e5a3ea5d4221ec56342023-12-01T08:31:55ZengTaylor & Francis GroupCogent Food & Agriculture2331-19322023-12-019110.1080/23311932.2023.2196871Fructan mixtures of Agave salmiana and chicory exhibit in vitro anticancer potential in human colon cells and prebiotic activityPaola Alvarez García0Luz Eugenia Alcántara Quintana1Miguel Ángel Ruiz Cabrera2Fidel Martínez Gutiérrez3Raúl González García4Alicia Grajales Lagunes5Faculty of Chemistry, Autonomous University of San Luis Potosí, San Luis Potosí, MéxicoCoordination for Innovation and Application of Science and Technology, Autonomous University of San Luis Potosí, San Luis Potosí, MéxicoFaculty of Chemistry, Autonomous University of San Luis Potosí, San Luis Potosí, MéxicoFaculty of Chemistry, Autonomous University of San Luis Potosí, San Luis Potosí, MéxicoFaculty of Chemistry, Autonomous University of San Luis Potosí, San Luis Potosí, MéxicoFaculty of Chemistry, Autonomous University of San Luis Potosí, San Luis Potosí, MéxicoAbstractAnticancer potential and a prebiotic effect of fructan mixtures from Agave salmiana (branched) and chicory inulin (linear) were evaluated to different mass fractions 1/0, 0/1, 0.5/0.5, 0.75/0.25, 0.25/0.75, respectively, in three concentrations: 2, 3.5, and 5 mg/mL. The viability, proliferation, and apoptosis of human colon cell lines, CRL1831 (healthy), HT29 (cancerous grade 1–2), and SW480 (cancerous grade 3–4), were evaluated by absorbance assays. Lactobacillus acidophilus and Bifidobacterium longum subsp. infantis growth was determined by spectrophotometry at 600 nm. The lactic acid and short-chain fatty acids (SCFAs) production was determined by the HPLC method. Fructan mixtures (inulin/agave, 0.25/0.75) showed a synergic effect on viability, proliferation, and decreased apoptosis in healthy colon cells (CRL1831) at 5 mg/mL. An increase (p < 0.05) of apoptosis in HT29 and SW480 was observed with inulin/agave (0/1) at 3.5 mg/mL and inulin/agave (0.5/0.5), respectively. The proliferation of L. acidophilus and B. longum subsp. infantis was better when the agave fructans were in a higher proportion than inulin. Lactic acid was the highest metabolite produced by L. acidophilus and acetic acid by B. longum subsp. infantis. Fructan mixtures induced SW480 and HT29 cells to apoptosis without toxic effects on healthy cells and improved bacteria proliferation and metabolite production.https://www.tandfonline.com/doi/10.1080/23311932.2023.2196871fructansSW480HT29CRL1831probiotic proliferationA. Salmiana |
| spellingShingle | Paola Alvarez García Luz Eugenia Alcántara Quintana Miguel Ángel Ruiz Cabrera Fidel Martínez Gutiérrez Raúl González García Alicia Grajales Lagunes Fructan mixtures of Agave salmiana and chicory exhibit in vitro anticancer potential in human colon cells and prebiotic activity Cogent Food & Agriculture fructans SW480 HT29 CRL1831 probiotic proliferation A. Salmiana |
| title | Fructan mixtures of Agave salmiana and chicory exhibit in vitro anticancer potential in human colon cells and prebiotic activity |
| title_full | Fructan mixtures of Agave salmiana and chicory exhibit in vitro anticancer potential in human colon cells and prebiotic activity |
| title_fullStr | Fructan mixtures of Agave salmiana and chicory exhibit in vitro anticancer potential in human colon cells and prebiotic activity |
| title_full_unstemmed | Fructan mixtures of Agave salmiana and chicory exhibit in vitro anticancer potential in human colon cells and prebiotic activity |
| title_short | Fructan mixtures of Agave salmiana and chicory exhibit in vitro anticancer potential in human colon cells and prebiotic activity |
| title_sort | fructan mixtures of agave salmiana and chicory exhibit in vitro anticancer potential in human colon cells and prebiotic activity |
| topic | fructans SW480 HT29 CRL1831 probiotic proliferation A. Salmiana |
| url | https://www.tandfonline.com/doi/10.1080/23311932.2023.2196871 |
| work_keys_str_mv | AT paolaalvarezgarcia fructanmixturesofagavesalmianaandchicoryexhibitinvitroanticancerpotentialinhumancoloncellsandprebioticactivity AT luzeugeniaalcantaraquintana fructanmixturesofagavesalmianaandchicoryexhibitinvitroanticancerpotentialinhumancoloncellsandprebioticactivity AT miguelangelruizcabrera fructanmixturesofagavesalmianaandchicoryexhibitinvitroanticancerpotentialinhumancoloncellsandprebioticactivity AT fidelmartinezgutierrez fructanmixturesofagavesalmianaandchicoryexhibitinvitroanticancerpotentialinhumancoloncellsandprebioticactivity AT raulgonzalezgarcia fructanmixturesofagavesalmianaandchicoryexhibitinvitroanticancerpotentialinhumancoloncellsandprebioticactivity AT aliciagrajaleslagunes fructanmixturesofagavesalmianaandchicoryexhibitinvitroanticancerpotentialinhumancoloncellsandprebioticactivity |