A novel portable flip-phone based visual behaviour assay for zebrafish
Abstract The optokinetic reflex (OKR) serves as a vital index for visual system development in early life, commonly observed within the first six months post-birth in humans. Zebrafish larvae offer a robust and convenient model for OKR studies due to their rapid development and manageable size. Exis...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-51001-7 |
| _version_ | 1797363333043585024 |
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| author | Vanessa Rodwell Annabel Birchall Ha-Jun Yoon Helen J. Kuht William H. J. Norton Mervyn G. Thomas |
| author_facet | Vanessa Rodwell Annabel Birchall Ha-Jun Yoon Helen J. Kuht William H. J. Norton Mervyn G. Thomas |
| author_sort | Vanessa Rodwell |
| collection | DOAJ |
| description | Abstract The optokinetic reflex (OKR) serves as a vital index for visual system development in early life, commonly observed within the first six months post-birth in humans. Zebrafish larvae offer a robust and convenient model for OKR studies due to their rapid development and manageable size. Existing OKR assays often involve cumbersome setups and offer limited portability. In this study, we present an innovative OKR assay that leverages the flexible screen of the Samsung Galaxy Z Flip to optimize setup and portability. We conducted paired slow-phase velocity measurements in 5-day post-fertilization (dpf) zebrafish larvae (n = 15), using both the novel flip-phone-based assay and a traditional liquid–crystal display (LCD) arena. Utilizing Bland–Altman plots, we assessed the agreement between the two methods. Both assays were efficacious in eliciting OKR, with eye movement analysis indicating high tracking precision in the flip-phone-based assay. No statistically significant difference was observed in slow-phase velocities between the two assays (p = 0.40). Our findings underscore the feasibility and non-inferiority of the flip-phone-based approach, offering streamlined assembly, enhanced portability, and the potential for cost-effective alternatives. This study contributes to the evolution of OKR assay methodologies, aligning them with emerging research paradigms. |
| first_indexed | 2024-03-08T16:19:54Z |
| format | Article |
| id | doaj.art-db1c9938021b443fa170dd331378292b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T16:19:54Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-db1c9938021b443fa170dd331378292b2024-01-07T12:26:25ZengNature PortfolioScientific Reports2045-23222024-01-011411710.1038/s41598-023-51001-7A novel portable flip-phone based visual behaviour assay for zebrafishVanessa Rodwell0Annabel Birchall1Ha-Jun Yoon2Helen J. Kuht3William H. J. Norton4Mervyn G. Thomas5The University of Leicester Ulverscroft Eye Unit, School of Psychology and Vision Sciences, University of Leicester, RKCSBThe University of Leicester Ulverscroft Eye Unit, School of Psychology and Vision Sciences, University of Leicester, RKCSBThe University of Leicester Ulverscroft Eye Unit, School of Psychology and Vision Sciences, University of Leicester, RKCSBThe University of Leicester Ulverscroft Eye Unit, School of Psychology and Vision Sciences, University of Leicester, RKCSBDepartment of Genetics and Genome Biology, University of LeicesterThe University of Leicester Ulverscroft Eye Unit, School of Psychology and Vision Sciences, University of Leicester, RKCSBAbstract The optokinetic reflex (OKR) serves as a vital index for visual system development in early life, commonly observed within the first six months post-birth in humans. Zebrafish larvae offer a robust and convenient model for OKR studies due to their rapid development and manageable size. Existing OKR assays often involve cumbersome setups and offer limited portability. In this study, we present an innovative OKR assay that leverages the flexible screen of the Samsung Galaxy Z Flip to optimize setup and portability. We conducted paired slow-phase velocity measurements in 5-day post-fertilization (dpf) zebrafish larvae (n = 15), using both the novel flip-phone-based assay and a traditional liquid–crystal display (LCD) arena. Utilizing Bland–Altman plots, we assessed the agreement between the two methods. Both assays were efficacious in eliciting OKR, with eye movement analysis indicating high tracking precision in the flip-phone-based assay. No statistically significant difference was observed in slow-phase velocities between the two assays (p = 0.40). Our findings underscore the feasibility and non-inferiority of the flip-phone-based approach, offering streamlined assembly, enhanced portability, and the potential for cost-effective alternatives. This study contributes to the evolution of OKR assay methodologies, aligning them with emerging research paradigms.https://doi.org/10.1038/s41598-023-51001-7 |
| spellingShingle | Vanessa Rodwell Annabel Birchall Ha-Jun Yoon Helen J. Kuht William H. J. Norton Mervyn G. Thomas A novel portable flip-phone based visual behaviour assay for zebrafish Scientific Reports |
| title | A novel portable flip-phone based visual behaviour assay for zebrafish |
| title_full | A novel portable flip-phone based visual behaviour assay for zebrafish |
| title_fullStr | A novel portable flip-phone based visual behaviour assay for zebrafish |
| title_full_unstemmed | A novel portable flip-phone based visual behaviour assay for zebrafish |
| title_short | A novel portable flip-phone based visual behaviour assay for zebrafish |
| title_sort | novel portable flip phone based visual behaviour assay for zebrafish |
| url | https://doi.org/10.1038/s41598-023-51001-7 |
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