Reverse genetics in virology: A double edged sword
Reverse genetics via targeted modification of gene sequences to obtain a phenotype and the inference of a gene's function or regulatory mechanism is widely used as a potent tool in viral biology and application. However, while reverse genetics has contributed significantly to our understanding...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Biosafety and Health |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590053622001240 |
| _version_ | 1811257582475542528 |
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| author | Hongyu Chen Hongqi Liu Xiaozhong Peng |
| author_facet | Hongyu Chen Hongqi Liu Xiaozhong Peng |
| author_sort | Hongyu Chen |
| collection | DOAJ |
| description | Reverse genetics via targeted modification of gene sequences to obtain a phenotype and the inference of a gene's function or regulatory mechanism is widely used as a potent tool in viral biology and application. However, while reverse genetics has contributed significantly to our understanding of molecular biology and the pathogenesis of viruses, its accessibility (operation) and openness (data) have raised many concerns regarding biosafety and biosecurity. In this review, we retrospectively examine the development of reverse genetics and its applications in virology, then emphasize global biosafety and biosecurity concerns regarding reverse genetics, and summarize global regulations, governance, and laws on reverse genetics. This review seeks to enhance our understanding and rational application of reverse genetics technology for the benefit of humankind. |
| first_indexed | 2024-04-12T17:58:50Z |
| format | Article |
| id | doaj.art-db1d686ea46f43849af719d50b53cb4e |
| institution | Directory Open Access Journal |
| issn | 2590-0536 |
| language | English |
| last_indexed | 2024-04-12T17:58:50Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosafety and Health |
| spelling | doaj.art-db1d686ea46f43849af719d50b53cb4e2022-12-22T03:22:14ZengElsevierBiosafety and Health2590-05362022-10-0145303313Reverse genetics in virology: A double edged swordHongyu Chen0Hongqi Liu1Xiaozhong Peng2Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical School, Kunming 650118, ChinaInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical School, Kunming 650118, ChinaInstitute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical School, Beijing 100051, China; Institute of Laboratory Animals Science, Chinese Academy of Medical Sciences and Peking Union Medical School, Beijing 100021, China; Corresponding author: Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical School, Beijing 100051, China.Reverse genetics via targeted modification of gene sequences to obtain a phenotype and the inference of a gene's function or regulatory mechanism is widely used as a potent tool in viral biology and application. However, while reverse genetics has contributed significantly to our understanding of molecular biology and the pathogenesis of viruses, its accessibility (operation) and openness (data) have raised many concerns regarding biosafety and biosecurity. In this review, we retrospectively examine the development of reverse genetics and its applications in virology, then emphasize global biosafety and biosecurity concerns regarding reverse genetics, and summarize global regulations, governance, and laws on reverse genetics. This review seeks to enhance our understanding and rational application of reverse genetics technology for the benefit of humankind.http://www.sciencedirect.com/science/article/pii/S2590053622001240Reverse geneticsVirologyDual use research of concernBiosafetyBiosecurityRegulation |
| spellingShingle | Hongyu Chen Hongqi Liu Xiaozhong Peng Reverse genetics in virology: A double edged sword Biosafety and Health Reverse genetics Virology Dual use research of concern Biosafety Biosecurity Regulation |
| title | Reverse genetics in virology: A double edged sword |
| title_full | Reverse genetics in virology: A double edged sword |
| title_fullStr | Reverse genetics in virology: A double edged sword |
| title_full_unstemmed | Reverse genetics in virology: A double edged sword |
| title_short | Reverse genetics in virology: A double edged sword |
| title_sort | reverse genetics in virology a double edged sword |
| topic | Reverse genetics Virology Dual use research of concern Biosafety Biosecurity Regulation |
| url | http://www.sciencedirect.com/science/article/pii/S2590053622001240 |
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