Metabarcoding of mycetangia from the Dendroctonus frontalis species complex (Curculionidae: Scolytinae) reveals diverse and functionally redundant fungal assemblages
Dendroctonus-bark beetles are associated with microbes that can detoxify terpenes, degrade complex molecules, supplement and recycle nutrients, fix nitrogen, produce semiochemicals, and regulate ecological interactions between microbes. Females of some Dendroctonus species harbor microbes in special...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-09-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.969230/full |
| _version_ | 1811261588073611264 |
|---|---|
| author | Karina Vazquez-Ortiz Rosa María Pineda-Mendoza Román González-Escobedo Thomas S. Davis Kevin F. Salazar Flor N. Rivera-Orduña Gerardo Zúñiga |
| author_facet | Karina Vazquez-Ortiz Rosa María Pineda-Mendoza Román González-Escobedo Thomas S. Davis Kevin F. Salazar Flor N. Rivera-Orduña Gerardo Zúñiga |
| author_sort | Karina Vazquez-Ortiz |
| collection | DOAJ |
| description | Dendroctonus-bark beetles are associated with microbes that can detoxify terpenes, degrade complex molecules, supplement and recycle nutrients, fix nitrogen, produce semiochemicals, and regulate ecological interactions between microbes. Females of some Dendroctonus species harbor microbes in specialized organs called mycetangia; yet little is known about the microbial diversity contained in these structures. Here, we use metabarcoding to characterize mycetangial fungi from beetle species in the Dendroctonus frontalis complex, and analyze variation in biodiversity of microbial assemblages between beetle species. Overall fungal diversity was represented by 4 phyla, 13 classes, 25 orders, 39 families, and 48 genera, including 33 filamentous fungi, and 15 yeasts. The most abundant genera were Entomocorticium, Candida, Ophiostoma-Sporothrix, Ogataea, Nakazawaea, Yamadazyma, Ceratocystiopsis, Grosmannia-Leptographium, Absidia, and Cyberlindnera. Analysis of α-diversity indicated that fungal assemblages of D. vitei showed the highest richness and diversity, whereas those associated with D. brevicomis and D. barberi had the lowest richness and diversity, respectively. Analysis of β-diversity showed clear differentiation in the assemblages associated with D. adjunctus, D. barberi, and D. brevicomis, but not between closely related species, including D. frontalis and D. mesoamericanus and D. mexicanus and D. vitei. A core mycobiome was not statistically identified; however, the genus Ceratocystiopsis was shared among seven beetle species. Interpretation of a tanglegram suggests evolutionary congruence between fungal assemblages and species of the D. frontalis complex. The presence of different amplicon sequence variants (ASVs) of the same genus in assemblages from species of the D. frontalis complex outlines the complexity of molecular networks, with the most complex assemblages identified from D. vitei, D. mesoamericanus, D. adjunctus, and D. frontalis. Analysis of functional variation of fungal assemblages indicated multiple trophic groupings, symbiotroph/saprotroph guilds represented with the highest frequency (∼31% of identified genera). These findings improve our knowledge about the diversity of mycetangial communities in species of the D. frontalis complex and suggest that minimal apparently specific assemblages are maintained and regulated within mycetangia. |
| first_indexed | 2024-04-12T19:07:10Z |
| format | Article |
| id | doaj.art-db1d71df520d4ff2a5017f2f4b431c00 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T19:07:10Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-db1d71df520d4ff2a5017f2f4b431c002022-12-22T03:19:59ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.969230969230Metabarcoding of mycetangia from the Dendroctonus frontalis species complex (Curculionidae: Scolytinae) reveals diverse and functionally redundant fungal assemblagesKarina Vazquez-Ortiz0Rosa María Pineda-Mendoza1Román González-Escobedo2Thomas S. Davis3Kevin F. Salazar4Flor N. Rivera-Orduña5Gerardo Zúñiga6Laboratorio de Variación Biológica y Evolución, Departamento de Zoología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, MexicoLaboratorio de Variación Biológica y Evolución, Departamento de Zoología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, MexicoLaboratorio de Microbiología, Facultad de Zootecnia y Ecología, Universidad Autónoma de Chihuahua, Chihuahua, MexicoDepartment of Forest and Rangeland Stewardship, Warner College of Natural Resources, Colorado State University, Fort Collins, CO, United StatesLaboratorio de Variación Biológica y Evolución, Departamento de Zoología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, MexicoLaboratorio de Ecología Microbiana, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, MexicoLaboratorio de Variación Biológica y Evolución, Departamento de Zoología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, MexicoDendroctonus-bark beetles are associated with microbes that can detoxify terpenes, degrade complex molecules, supplement and recycle nutrients, fix nitrogen, produce semiochemicals, and regulate ecological interactions between microbes. Females of some Dendroctonus species harbor microbes in specialized organs called mycetangia; yet little is known about the microbial diversity contained in these structures. Here, we use metabarcoding to characterize mycetangial fungi from beetle species in the Dendroctonus frontalis complex, and analyze variation in biodiversity of microbial assemblages between beetle species. Overall fungal diversity was represented by 4 phyla, 13 classes, 25 orders, 39 families, and 48 genera, including 33 filamentous fungi, and 15 yeasts. The most abundant genera were Entomocorticium, Candida, Ophiostoma-Sporothrix, Ogataea, Nakazawaea, Yamadazyma, Ceratocystiopsis, Grosmannia-Leptographium, Absidia, and Cyberlindnera. Analysis of α-diversity indicated that fungal assemblages of D. vitei showed the highest richness and diversity, whereas those associated with D. brevicomis and D. barberi had the lowest richness and diversity, respectively. Analysis of β-diversity showed clear differentiation in the assemblages associated with D. adjunctus, D. barberi, and D. brevicomis, but not between closely related species, including D. frontalis and D. mesoamericanus and D. mexicanus and D. vitei. A core mycobiome was not statistically identified; however, the genus Ceratocystiopsis was shared among seven beetle species. Interpretation of a tanglegram suggests evolutionary congruence between fungal assemblages and species of the D. frontalis complex. The presence of different amplicon sequence variants (ASVs) of the same genus in assemblages from species of the D. frontalis complex outlines the complexity of molecular networks, with the most complex assemblages identified from D. vitei, D. mesoamericanus, D. adjunctus, and D. frontalis. Analysis of functional variation of fungal assemblages indicated multiple trophic groupings, symbiotroph/saprotroph guilds represented with the highest frequency (∼31% of identified genera). These findings improve our knowledge about the diversity of mycetangial communities in species of the D. frontalis complex and suggest that minimal apparently specific assemblages are maintained and regulated within mycetangia.https://www.frontiersin.org/articles/10.3389/fmicb.2022.969230/fullmycetangiamycobiomeITSsymbiosisbark beetlesDendroctonus |
| spellingShingle | Karina Vazquez-Ortiz Rosa María Pineda-Mendoza Román González-Escobedo Thomas S. Davis Kevin F. Salazar Flor N. Rivera-Orduña Gerardo Zúñiga Metabarcoding of mycetangia from the Dendroctonus frontalis species complex (Curculionidae: Scolytinae) reveals diverse and functionally redundant fungal assemblages Frontiers in Microbiology mycetangia mycobiome ITS symbiosis bark beetles Dendroctonus |
| title | Metabarcoding of mycetangia from the Dendroctonus frontalis species complex (Curculionidae: Scolytinae) reveals diverse and functionally redundant fungal assemblages |
| title_full | Metabarcoding of mycetangia from the Dendroctonus frontalis species complex (Curculionidae: Scolytinae) reveals diverse and functionally redundant fungal assemblages |
| title_fullStr | Metabarcoding of mycetangia from the Dendroctonus frontalis species complex (Curculionidae: Scolytinae) reveals diverse and functionally redundant fungal assemblages |
| title_full_unstemmed | Metabarcoding of mycetangia from the Dendroctonus frontalis species complex (Curculionidae: Scolytinae) reveals diverse and functionally redundant fungal assemblages |
| title_short | Metabarcoding of mycetangia from the Dendroctonus frontalis species complex (Curculionidae: Scolytinae) reveals diverse and functionally redundant fungal assemblages |
| title_sort | metabarcoding of mycetangia from the dendroctonus frontalis species complex curculionidae scolytinae reveals diverse and functionally redundant fungal assemblages |
| topic | mycetangia mycobiome ITS symbiosis bark beetles Dendroctonus |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.969230/full |
| work_keys_str_mv | AT karinavazquezortiz metabarcodingofmycetangiafromthedendroctonusfrontalisspeciescomplexcurculionidaescolytinaerevealsdiverseandfunctionallyredundantfungalassemblages AT rosamariapinedamendoza metabarcodingofmycetangiafromthedendroctonusfrontalisspeciescomplexcurculionidaescolytinaerevealsdiverseandfunctionallyredundantfungalassemblages AT romangonzalezescobedo metabarcodingofmycetangiafromthedendroctonusfrontalisspeciescomplexcurculionidaescolytinaerevealsdiverseandfunctionallyredundantfungalassemblages AT thomassdavis metabarcodingofmycetangiafromthedendroctonusfrontalisspeciescomplexcurculionidaescolytinaerevealsdiverseandfunctionallyredundantfungalassemblages AT kevinfsalazar metabarcodingofmycetangiafromthedendroctonusfrontalisspeciescomplexcurculionidaescolytinaerevealsdiverseandfunctionallyredundantfungalassemblages AT flornriveraorduna metabarcodingofmycetangiafromthedendroctonusfrontalisspeciescomplexcurculionidaescolytinaerevealsdiverseandfunctionallyredundantfungalassemblages AT gerardozuniga metabarcodingofmycetangiafromthedendroctonusfrontalisspeciescomplexcurculionidaescolytinaerevealsdiverseandfunctionallyredundantfungalassemblages |