Development of an Antimicrobial Peptide-based Biosensor for the Monitoring of Bacterial Contaminations
Nisin is a cationic amphiphilic peptide consisting of 34 amino acids with a cluster of hydrophobic residues at the N-terminus and hydrophilic residues at the C-terminus. The mechanism of its action is based on its ability to attach to the bacteria cell membrane before to then cause cell lysis. This...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
AIDIC Servizi S.r.l.
2019-06-01
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| Series: | Chemical Engineering Transactions |
| Online Access: | https://www.cetjournal.it/index.php/cet/article/view/9669 |
| _version_ | 1818658148712448000 |
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| author | Donatella Albanese Francesca Garofalo Roberto Pilloton Salvatore Capo Francesca Malvano |
| author_facet | Donatella Albanese Francesca Garofalo Roberto Pilloton Salvatore Capo Francesca Malvano |
| author_sort | Donatella Albanese |
| collection | DOAJ |
| description | Nisin is a cationic amphiphilic peptide consisting of 34 amino acids with a cluster of hydrophobic residues at the N-terminus and hydrophilic residues at the C-terminus. The mechanism of its action is based on its ability to attach to the bacteria cell membrane before to then cause cell lysis. This antimicrobial peptide was used as biological molecule for the development of a novel impedimetric label-free biosensor for the monitoring of bacterial contamination. The binding affinities of Nisin immobilized with its N-terminus and C-terminus were compared, highlighting the capability of the last configuration to obtain the best analytical performance of the developed biosensor when non-pathogenic Escherichia Coli O157:H7 and Listeria innocua cells were investigated. |
| first_indexed | 2024-12-17T03:52:46Z |
| format | Article |
| id | doaj.art-db1f094420634c218d8918db7c55beb2 |
| institution | Directory Open Access Journal |
| issn | 2283-9216 |
| language | English |
| last_indexed | 2024-12-17T03:52:46Z |
| publishDate | 2019-06-01 |
| publisher | AIDIC Servizi S.r.l. |
| record_format | Article |
| series | Chemical Engineering Transactions |
| spelling | doaj.art-db1f094420634c218d8918db7c55beb22022-12-21T22:04:42ZengAIDIC Servizi S.r.l.Chemical Engineering Transactions2283-92162019-06-017510.3303/CET1975011Development of an Antimicrobial Peptide-based Biosensor for the Monitoring of Bacterial ContaminationsDonatella AlbaneseFrancesca GarofaloRoberto PillotonSalvatore CapoFrancesca MalvanoNisin is a cationic amphiphilic peptide consisting of 34 amino acids with a cluster of hydrophobic residues at the N-terminus and hydrophilic residues at the C-terminus. The mechanism of its action is based on its ability to attach to the bacteria cell membrane before to then cause cell lysis. This antimicrobial peptide was used as biological molecule for the development of a novel impedimetric label-free biosensor for the monitoring of bacterial contamination. The binding affinities of Nisin immobilized with its N-terminus and C-terminus were compared, highlighting the capability of the last configuration to obtain the best analytical performance of the developed biosensor when non-pathogenic Escherichia Coli O157:H7 and Listeria innocua cells were investigated.https://www.cetjournal.it/index.php/cet/article/view/9669 |
| spellingShingle | Donatella Albanese Francesca Garofalo Roberto Pilloton Salvatore Capo Francesca Malvano Development of an Antimicrobial Peptide-based Biosensor for the Monitoring of Bacterial Contaminations Chemical Engineering Transactions |
| title | Development of an Antimicrobial Peptide-based Biosensor for the Monitoring of Bacterial Contaminations |
| title_full | Development of an Antimicrobial Peptide-based Biosensor for the Monitoring of Bacterial Contaminations |
| title_fullStr | Development of an Antimicrobial Peptide-based Biosensor for the Monitoring of Bacterial Contaminations |
| title_full_unstemmed | Development of an Antimicrobial Peptide-based Biosensor for the Monitoring of Bacterial Contaminations |
| title_short | Development of an Antimicrobial Peptide-based Biosensor for the Monitoring of Bacterial Contaminations |
| title_sort | development of an antimicrobial peptide based biosensor for the monitoring of bacterial contaminations |
| url | https://www.cetjournal.it/index.php/cet/article/view/9669 |
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