Expressions of miRNAs in Papillary Thyroid Carcinoma and Their Associations with the BRAF Mutation and Clinicopathological Features
Objectives The microRNAs (miRNAs) are known to be commonly expressed in papillary thyroid carcinoma. The BRAFV600E mutation is the most common genetic mutation in thyroid cancer. The main aim of this study was to determine the possible association between expression of the three miRNAs and that of B...
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| Format: | Article |
| Language: | English |
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Kosin University College of Medicine
2020-06-01
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| Series: | Kosin Medical Journal |
| Subjects: | |
| Online Access: | http://www.kosinmedj.org/upload/pdf/kmj-35-1-1.pdf |
| _version_ | 1811192634022035456 |
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| author | Song I Yang Young Sik Choi |
| author_facet | Song I Yang Young Sik Choi |
| author_sort | Song I Yang |
| collection | DOAJ |
| description | Objectives The microRNAs (miRNAs) are known to be commonly expressed in papillary thyroid carcinoma. The BRAFV600E mutation is the most common genetic mutation in thyroid cancer. The main aim of this study was to determine the possible association between expression of the three miRNAs and that of BRAFV600E mutation and the clinicopathological features in papillary thyroid carcinoma. Methods This study was conducted on 51 paraffin-embedded tissues (42 thyroid cancer, 9 benign tumor) obtained from patients undergoing thyroidectomy at the Endocrine Center of OOO University Hospital. Results miRNAs expression was significantly high in patients with cervical lymph node metastasis and advanced TNM stage. In addition, miR-146b expression levels were significantly higher in papillary thyroid carcinoma patients with BRAFV600E mutation. The relative quantification (2-ΔΔCt) of miR-146b was also high among the miRNAs. Individually, the AUCs for miRNA-146b was 0.923 (cutoff value −1.97, sensitivity 88.9%, specificity 85.7%). Conclusions Especially, expression of miR-146b increased higher in PTC patients with BRAFV600E mutation. These findings showed a role of miR-146b as potential biomarkers in differentiating PTC from benign tumor and as a prognostic indicator of PTCs. Further investigation will need for the roles of miRNAs in the pathogenesis of papillary thyroid carcinomas. |
| first_indexed | 2024-04-11T23:55:39Z |
| format | Article |
| id | doaj.art-db2041b4eacb4dbbaf821390b1134f58 |
| institution | Directory Open Access Journal |
| issn | 2005-9531 2586-7024 |
| language | English |
| last_indexed | 2024-04-11T23:55:39Z |
| publishDate | 2020-06-01 |
| publisher | Kosin University College of Medicine |
| record_format | Article |
| series | Kosin Medical Journal |
| spelling | doaj.art-db2041b4eacb4dbbaf821390b1134f582022-12-22T03:56:22ZengKosin University College of MedicineKosin Medical Journal2005-95312586-70242020-06-0135111410.7180/kmj.2020.35.1.1216Expressions of miRNAs in Papillary Thyroid Carcinoma and Their Associations with the BRAF Mutation and Clinicopathological FeaturesSong I Yang0Young Sik Choi1 Department of Surgery, College of Medicine, Kosin University, Busan, Korea Department of Internal Medicine, College of Medicine, Kosin University, Busan, KoreaObjectives The microRNAs (miRNAs) are known to be commonly expressed in papillary thyroid carcinoma. The BRAFV600E mutation is the most common genetic mutation in thyroid cancer. The main aim of this study was to determine the possible association between expression of the three miRNAs and that of BRAFV600E mutation and the clinicopathological features in papillary thyroid carcinoma. Methods This study was conducted on 51 paraffin-embedded tissues (42 thyroid cancer, 9 benign tumor) obtained from patients undergoing thyroidectomy at the Endocrine Center of OOO University Hospital. Results miRNAs expression was significantly high in patients with cervical lymph node metastasis and advanced TNM stage. In addition, miR-146b expression levels were significantly higher in papillary thyroid carcinoma patients with BRAFV600E mutation. The relative quantification (2-ΔΔCt) of miR-146b was also high among the miRNAs. Individually, the AUCs for miRNA-146b was 0.923 (cutoff value −1.97, sensitivity 88.9%, specificity 85.7%). Conclusions Especially, expression of miR-146b increased higher in PTC patients with BRAFV600E mutation. These findings showed a role of miR-146b as potential biomarkers in differentiating PTC from benign tumor and as a prognostic indicator of PTCs. Further investigation will need for the roles of miRNAs in the pathogenesis of papillary thyroid carcinomas.http://www.kosinmedj.org/upload/pdf/kmj-35-1-1.pdfbraf mutationmicrornapapillary thyroid cancer |
| spellingShingle | Song I Yang Young Sik Choi Expressions of miRNAs in Papillary Thyroid Carcinoma and Their Associations with the BRAF Mutation and Clinicopathological Features Kosin Medical Journal braf mutation microrna papillary thyroid cancer |
| title | Expressions of miRNAs in Papillary Thyroid Carcinoma and Their Associations with the BRAF Mutation and Clinicopathological Features |
| title_full | Expressions of miRNAs in Papillary Thyroid Carcinoma and Their Associations with the BRAF Mutation and Clinicopathological Features |
| title_fullStr | Expressions of miRNAs in Papillary Thyroid Carcinoma and Their Associations with the BRAF Mutation and Clinicopathological Features |
| title_full_unstemmed | Expressions of miRNAs in Papillary Thyroid Carcinoma and Their Associations with the BRAF Mutation and Clinicopathological Features |
| title_short | Expressions of miRNAs in Papillary Thyroid Carcinoma and Their Associations with the BRAF Mutation and Clinicopathological Features |
| title_sort | expressions of mirnas in papillary thyroid carcinoma and their associations with the braf mutation and clinicopathological features |
| topic | braf mutation microrna papillary thyroid cancer |
| url | http://www.kosinmedj.org/upload/pdf/kmj-35-1-1.pdf |
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