<i>ZmDREB2.9</i> Gene in Maize (<i>Zea mays</i> L.): Genome-Wide Identification, Characterization, Expression, and Stress Response
Dehydration-responsive element-binding (DREB) transcription factors of the A2 subfamily play key roles in plant stress responses. In this study, we identified and characterized a new A2-type <i>DREB</i> gene, <i>ZmDREB2.9</i>, in the <i>Zea mays</i> cv. B73 genome...
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MDPI AG
2022-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/22/3060 |
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| author | Mikhail A. Filyushin Elena Z. Kochieva Anna V. Shchennikova |
| author_facet | Mikhail A. Filyushin Elena Z. Kochieva Anna V. Shchennikova |
| author_sort | Mikhail A. Filyushin |
| collection | DOAJ |
| description | Dehydration-responsive element-binding (DREB) transcription factors of the A2 subfamily play key roles in plant stress responses. In this study, we identified and characterized a new A2-type <i>DREB</i> gene, <i>ZmDREB2.9</i>, in the <i>Zea mays</i> cv. B73 genome and compared its expression profile with those of the known A2-type maize genes <i>ZmDREB2.1–2.8</i>. <i>ZmDREB2.9</i> was mapped to chromosome 8, contained 18 predicted hormone- and stress-responsive <i>cis</i>-elements in the promoter, and had two splice isoforms: short <i>ZmDREB2.9-S</i> preferentially expressed in the leaves, embryos, and endosperm and long <i>ZmDREB2.9-L</i> expressed mostly in the male flowers, stamens, and ovaries. Phylogenetically, ZmDREB2.9 was closer to <i>A. thaliana</i> DREB2A than the other ZmDREB2 factors. <i>ZmDREB2.9-S</i>, <i>ZmDREB2.2</i>, and <i>ZmDREB2.1/2A</i> were upregulated in response to cold, drought, and abscisic acid and may play redundant roles in maize stress resistance. <i>ZmDREB2.3</i>, <i>ZmDREB2.4</i>, and <i>ZmDREB2.6</i> were not expressed in seedlings and could be pseudogenes. <i>ZmDREB2.7</i> and <i>ZmDREB2.8</i> showed similar transcript accumulation in response to cold and abscisic acid and could be functionally redundant. Our results provide new data on <i>Z. mays</i> DREB2 factors, which can be used for further functional studies as well as in breeding programs to improve maize stress tolerance. |
| first_indexed | 2024-03-09T18:04:24Z |
| format | Article |
| id | doaj.art-db221424d9b54060b0a43565646211a0 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T18:04:24Z |
| publishDate | 2022-11-01 |
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| series | Plants |
| spelling | doaj.art-db221424d9b54060b0a43565646211a02023-11-24T09:38:59ZengMDPI AGPlants2223-77472022-11-011122306010.3390/plants11223060<i>ZmDREB2.9</i> Gene in Maize (<i>Zea mays</i> L.): Genome-Wide Identification, Characterization, Expression, and Stress ResponseMikhail A. Filyushin0Elena Z. Kochieva1Anna V. Shchennikova2Research Center of Biotechnology, Institute of Bioengineering, Russian Academy of Sciences, Leninsky Ave. 33, Bld. 2, Moscow 119071, RussiaResearch Center of Biotechnology, Institute of Bioengineering, Russian Academy of Sciences, Leninsky Ave. 33, Bld. 2, Moscow 119071, RussiaResearch Center of Biotechnology, Institute of Bioengineering, Russian Academy of Sciences, Leninsky Ave. 33, Bld. 2, Moscow 119071, RussiaDehydration-responsive element-binding (DREB) transcription factors of the A2 subfamily play key roles in plant stress responses. In this study, we identified and characterized a new A2-type <i>DREB</i> gene, <i>ZmDREB2.9</i>, in the <i>Zea mays</i> cv. B73 genome and compared its expression profile with those of the known A2-type maize genes <i>ZmDREB2.1–2.8</i>. <i>ZmDREB2.9</i> was mapped to chromosome 8, contained 18 predicted hormone- and stress-responsive <i>cis</i>-elements in the promoter, and had two splice isoforms: short <i>ZmDREB2.9-S</i> preferentially expressed in the leaves, embryos, and endosperm and long <i>ZmDREB2.9-L</i> expressed mostly in the male flowers, stamens, and ovaries. Phylogenetically, ZmDREB2.9 was closer to <i>A. thaliana</i> DREB2A than the other ZmDREB2 factors. <i>ZmDREB2.9-S</i>, <i>ZmDREB2.2</i>, and <i>ZmDREB2.1/2A</i> were upregulated in response to cold, drought, and abscisic acid and may play redundant roles in maize stress resistance. <i>ZmDREB2.3</i>, <i>ZmDREB2.4</i>, and <i>ZmDREB2.6</i> were not expressed in seedlings and could be pseudogenes. <i>ZmDREB2.7</i> and <i>ZmDREB2.8</i> showed similar transcript accumulation in response to cold and abscisic acid and could be functionally redundant. Our results provide new data on <i>Z. mays</i> DREB2 factors, which can be used for further functional studies as well as in breeding programs to improve maize stress tolerance.https://www.mdpi.com/2223-7747/11/22/3060<i>Zea mays</i> L.DREB proteinsgene structuregene expressionabiotic stress |
| spellingShingle | Mikhail A. Filyushin Elena Z. Kochieva Anna V. Shchennikova <i>ZmDREB2.9</i> Gene in Maize (<i>Zea mays</i> L.): Genome-Wide Identification, Characterization, Expression, and Stress Response Plants <i>Zea mays</i> L. DREB proteins gene structure gene expression abiotic stress |
| title | <i>ZmDREB2.9</i> Gene in Maize (<i>Zea mays</i> L.): Genome-Wide Identification, Characterization, Expression, and Stress Response |
| title_full | <i>ZmDREB2.9</i> Gene in Maize (<i>Zea mays</i> L.): Genome-Wide Identification, Characterization, Expression, and Stress Response |
| title_fullStr | <i>ZmDREB2.9</i> Gene in Maize (<i>Zea mays</i> L.): Genome-Wide Identification, Characterization, Expression, and Stress Response |
| title_full_unstemmed | <i>ZmDREB2.9</i> Gene in Maize (<i>Zea mays</i> L.): Genome-Wide Identification, Characterization, Expression, and Stress Response |
| title_short | <i>ZmDREB2.9</i> Gene in Maize (<i>Zea mays</i> L.): Genome-Wide Identification, Characterization, Expression, and Stress Response |
| title_sort | i zmdreb2 9 i gene in maize i zea mays i l genome wide identification characterization expression and stress response |
| topic | <i>Zea mays</i> L. DREB proteins gene structure gene expression abiotic stress |
| url | https://www.mdpi.com/2223-7747/11/22/3060 |
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