Effect of antileukotriene treatment on capsular contracture: an experimental study
Introduction: 40% of patients undergoing radiotherapy after breast reconstruction by silicone prosthesis implant may develop prosthesis encapsulation. Several strategies have already been tested to prevent capsule contracture with unsatisfactory results. This study analyzed the effect of topical ant...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Cirurgia Plástica
2021-09-01
|
| Series: | Revista Brasileira de Cirurgia Plástica |
| Subjects: | |
| Online Access: | http://www.rbcp.org.br/export-pdf/2984/en_v36n3a08.pdf |
| _version_ | 1827607672697913344 |
|---|---|
| author | Cristina Pires Camargo Heloisa Andrade Carvalho Marcia Saldanha Kubrusly Sofia Amaral Medeiros Julio Morais Besteiro Rolf Gemperli |
| author_facet | Cristina Pires Camargo Heloisa Andrade Carvalho Marcia Saldanha Kubrusly Sofia Amaral Medeiros Julio Morais Besteiro Rolf Gemperli |
| author_sort | Cristina Pires Camargo |
| collection | DOAJ |
| description | Introduction: 40% of patients undergoing radiotherapy after breast reconstruction by silicone prosthesis implant may develop prosthesis encapsulation. Several strategies have already been tested to prevent capsule contracture with unsatisfactory results. This study analyzed the effect of topical antileukotriene (AL) on capsular contracture formation in rats with silicone implants associated with irradiation.
Methods: Silicone blocks were implanted in the dorsal region in 20 female rats Wistar with weights ranging from 200-250g. The animals were divided into two groups: control (injection of 0.9% saline solution into the tissue around the implant) and intervention group (injection of 10mg of AL into the tissue around the implant). Immediately after surgery, the animals were irradiated with a single dose of 10Gy. After two months, we collected capsule samples for histological analysis and gene expression analysis of the following biomarkers: iNOS, VEGF-a and MMP-9.
Results: Vascular density was lower in the AL group when compared to the control group (55.4±30.0 vs. 81.8±26.7, p=0.05, respectively). Similarly, VEGF-a had the same behavior (control group - 0.34±0.1 vs. group Al - 0.02±0.001, p=0.04).
Conclusion: This study suggested that treatment with AL decreases angiogenesis in animals submitted to silicone implants and underwent radiotherapy. |
| first_indexed | 2024-03-09T06:58:33Z |
| format | Article |
| id | doaj.art-db23f19c43a740019e9af9dac31e3bb3 |
| institution | Directory Open Access Journal |
| issn | 1983-5175 2177-1235 |
| language | English |
| last_indexed | 2024-03-09T06:58:33Z |
| publishDate | 2021-09-01 |
| publisher | Sociedade Brasileira de Cirurgia Plástica |
| record_format | Article |
| series | Revista Brasileira de Cirurgia Plástica |
| spelling | doaj.art-db23f19c43a740019e9af9dac31e3bb32023-12-03T09:59:48ZengSociedade Brasileira de Cirurgia PlásticaRevista Brasileira de Cirurgia Plástica1983-51752177-12352021-09-01360328128610.5935/2177-1235.2021RBCP0092Effect of antileukotriene treatment on capsular contracture: an experimental studyCristina Pires Camargo0Heloisa Andrade Carvalho1Marcia Saldanha Kubrusly2Sofia Amaral Medeiros3Julio Morais Besteiro4Rolf Gemperli5University of São Paulo, São Paulo, SP, BrazilUniversity of São Paulo, São Paulo, SP, BrazilUniversity of São Paulo, São Paulo, SP, BrazilUniversity of São Paulo, São Paulo, SP, BrazilUniversity of São Paulo, São Paulo, SP, BrazilUniversity of São Paulo, São Paulo, SP, BrazilIntroduction: 40% of patients undergoing radiotherapy after breast reconstruction by silicone prosthesis implant may develop prosthesis encapsulation. Several strategies have already been tested to prevent capsule contracture with unsatisfactory results. This study analyzed the effect of topical antileukotriene (AL) on capsular contracture formation in rats with silicone implants associated with irradiation. Methods: Silicone blocks were implanted in the dorsal region in 20 female rats Wistar with weights ranging from 200-250g. The animals were divided into two groups: control (injection of 0.9% saline solution into the tissue around the implant) and intervention group (injection of 10mg of AL into the tissue around the implant). Immediately after surgery, the animals were irradiated with a single dose of 10Gy. After two months, we collected capsule samples for histological analysis and gene expression analysis of the following biomarkers: iNOS, VEGF-a and MMP-9. Results: Vascular density was lower in the AL group when compared to the control group (55.4±30.0 vs. 81.8±26.7, p=0.05, respectively). Similarly, VEGF-a had the same behavior (control group - 0.34±0.1 vs. group Al - 0.02±0.001, p=0.04). Conclusion: This study suggested that treatment with AL decreases angiogenesis in animals submitted to silicone implants and underwent radiotherapy.http://www.rbcp.org.br/export-pdf/2984/en_v36n3a08.pdfradiotherapymousecapsular contracture in implantvascular endothelium growth factor amatrix metalloproteinases inhibitors |
| spellingShingle | Cristina Pires Camargo Heloisa Andrade Carvalho Marcia Saldanha Kubrusly Sofia Amaral Medeiros Julio Morais Besteiro Rolf Gemperli Effect of antileukotriene treatment on capsular contracture: an experimental study Revista Brasileira de Cirurgia Plástica radiotherapy mouse capsular contracture in implant vascular endothelium growth factor a matrix metalloproteinases inhibitors |
| title | Effect of antileukotriene treatment on capsular
contracture: an experimental study |
| title_full | Effect of antileukotriene treatment on capsular
contracture: an experimental study |
| title_fullStr | Effect of antileukotriene treatment on capsular
contracture: an experimental study |
| title_full_unstemmed | Effect of antileukotriene treatment on capsular
contracture: an experimental study |
| title_short | Effect of antileukotriene treatment on capsular
contracture: an experimental study |
| title_sort | effect of antileukotriene treatment on capsular contracture an experimental study |
| topic | radiotherapy mouse capsular contracture in implant vascular endothelium growth factor a matrix metalloproteinases inhibitors |
| url | http://www.rbcp.org.br/export-pdf/2984/en_v36n3a08.pdf |
| work_keys_str_mv | AT cristinapirescamargo effectofantileukotrienetreatmentoncapsularcontractureanexperimentalstudy AT heloisaandradecarvalho effectofantileukotrienetreatmentoncapsularcontractureanexperimentalstudy AT marciasaldanhakubrusly effectofantileukotrienetreatmentoncapsularcontractureanexperimentalstudy AT sofiaamaralmedeiros effectofantileukotrienetreatmentoncapsularcontractureanexperimentalstudy AT juliomoraisbesteiro effectofantileukotrienetreatmentoncapsularcontractureanexperimentalstudy AT rolfgemperli effectofantileukotrienetreatmentoncapsularcontractureanexperimentalstudy |