The Association of Genetic Variant rs8506C>T at miR-526b Binding Site in the LincRNA-NR_024015 Exon with Breast Cancer Risk
Background and Aim: The long noncoding RNAs (lncRNAs) is an important type of RNAs that can regulate gene expression and, therefore, are involved in the development of various cancers. The genome-wide association study (GWAS) is used to identify phenotype-related loci within non-coding regions. Howe...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Arak Medical University
2020-05-01
|
| Series: | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| Subjects: | |
| Online Access: | http://jams.arakmu.ac.ir/article-1-6111-en.html |
| _version_ | 1828759049719513088 |
|---|---|
| author | Fatemeh Tavakoli Somayeh Reiisi |
| author_facet | Fatemeh Tavakoli Somayeh Reiisi |
| author_sort | Fatemeh Tavakoli |
| collection | DOAJ |
| description | Background and Aim: The long noncoding RNAs (lncRNAs) is an important type of RNAs that can regulate gene expression and, therefore, are involved in the development of various cancers. The genome-wide association study (GWAS) is used to identify phenotype-related loci within non-coding regions. However, the biological functions and exact relationships between phenotype-related loci and lncRNAs have not fully been identified. No study was found on the relationship between rs8506C>T polymorphisms in the lincRNA-NR_024015 exon and breast cancer susceptibility and clinical factors. Therefore, the present study aimed to evaluate the effect of polymorphism rs8506C>T on the breast cancer risk.
Methods & Materials: In this case-control study, participants were 120 patients with breast cancer, 120 healthy controls. The genetic variant was genotyped by using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR–RFLP) method. Interactions between the polymorphism and clinical factors were further evaluated, and Odds Ratio (OR) was measured for risk assessment.
Ethical Considerations: This study has been approved by the Research Ethics Committee at Shahrekord University of Medical Sciences (code:????) .
Results: There was a correlation between rs8506 C>T polymorphism and breast cancer risk in the dominant model (CC and CT+TT genotypes; P=0.027; OR=1.84; 95% CI: 1.067‐3.201). In the co-dominant model, CT genotype had a statistically significant association with breast cancer risk (P=0.038). Subjects with T allele in the rs8506 polymorphism had an increased risk of breast cancer (OR=1.69; 95% CI: 1.047-2.736; P=0.031). No relationship between rs8506 polymorphism and clinical factors including metastasis, tumor grade, and Human Epidermal Growth Factor Receptor 2 (HER2) status was observed.
Conclusion: Genetic variant rs8506 C>T polymorphism in the lincRNA-NR_024015 exon may contribute to the breast cancer risk. Allele T in this variant confers an increased risk of breast cancer. Further functional analyses are required to detect the detailed mechanism underlying the observed association. |
| first_indexed | 2024-12-11T00:52:23Z |
| format | Article |
| id | doaj.art-db2622dead944ddbb7838be31219abdc |
| institution | Directory Open Access Journal |
| issn | 1735-5338 2008-644X |
| language | fas |
| last_indexed | 2024-12-11T00:52:23Z |
| publishDate | 2020-05-01 |
| publisher | Arak Medical University |
| record_format | Article |
| series | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| spelling | doaj.art-db2622dead944ddbb7838be31219abdc2022-12-22T01:26:36ZfasArak Medical UniversityMajallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk1735-53382008-644X2020-05-01232222235The Association of Genetic Variant rs8506C>T at miR-526b Binding Site in the LincRNA-NR_024015 Exon with Breast Cancer RiskFatemeh Tavakoli0Somayeh Reiisi1 MSc. Student, Department of Genetics, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran. Assistant Professor, Department of Genetics, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran. Background and Aim: The long noncoding RNAs (lncRNAs) is an important type of RNAs that can regulate gene expression and, therefore, are involved in the development of various cancers. The genome-wide association study (GWAS) is used to identify phenotype-related loci within non-coding regions. However, the biological functions and exact relationships between phenotype-related loci and lncRNAs have not fully been identified. No study was found on the relationship between rs8506C>T polymorphisms in the lincRNA-NR_024015 exon and breast cancer susceptibility and clinical factors. Therefore, the present study aimed to evaluate the effect of polymorphism rs8506C>T on the breast cancer risk. Methods & Materials: In this case-control study, participants were 120 patients with breast cancer, 120 healthy controls. The genetic variant was genotyped by using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR–RFLP) method. Interactions between the polymorphism and clinical factors were further evaluated, and Odds Ratio (OR) was measured for risk assessment. Ethical Considerations: This study has been approved by the Research Ethics Committee at Shahrekord University of Medical Sciences (code:????) . Results: There was a correlation between rs8506 C>T polymorphism and breast cancer risk in the dominant model (CC and CT+TT genotypes; P=0.027; OR=1.84; 95% CI: 1.067‐3.201). In the co-dominant model, CT genotype had a statistically significant association with breast cancer risk (P=0.038). Subjects with T allele in the rs8506 polymorphism had an increased risk of breast cancer (OR=1.69; 95% CI: 1.047-2.736; P=0.031). No relationship between rs8506 polymorphism and clinical factors including metastasis, tumor grade, and Human Epidermal Growth Factor Receptor 2 (HER2) status was observed. Conclusion: Genetic variant rs8506 C>T polymorphism in the lincRNA-NR_024015 exon may contribute to the breast cancer risk. Allele T in this variant confers an increased risk of breast cancer. Further functional analyses are required to detect the detailed mechanism underlying the observed association.http://jams.arakmu.ac.ir/article-1-6111-en.htmlpolymorphismlincrna-nr_024015 exonmir-526b binding sitebreast cancer |
| spellingShingle | Fatemeh Tavakoli Somayeh Reiisi The Association of Genetic Variant rs8506C>T at miR-526b Binding Site in the LincRNA-NR_024015 Exon with Breast Cancer Risk Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk polymorphism lincrna-nr_024015 exon mir-526b binding site breast cancer |
| title | The Association of Genetic Variant rs8506C>T at miR-526b Binding Site in the LincRNA-NR_024015 Exon with Breast Cancer Risk |
| title_full | The Association of Genetic Variant rs8506C>T at miR-526b Binding Site in the LincRNA-NR_024015 Exon with Breast Cancer Risk |
| title_fullStr | The Association of Genetic Variant rs8506C>T at miR-526b Binding Site in the LincRNA-NR_024015 Exon with Breast Cancer Risk |
| title_full_unstemmed | The Association of Genetic Variant rs8506C>T at miR-526b Binding Site in the LincRNA-NR_024015 Exon with Breast Cancer Risk |
| title_short | The Association of Genetic Variant rs8506C>T at miR-526b Binding Site in the LincRNA-NR_024015 Exon with Breast Cancer Risk |
| title_sort | association of genetic variant rs8506c t at mir 526b binding site in the lincrna nr 024015 exon with breast cancer risk |
| topic | polymorphism lincrna-nr_024015 exon mir-526b binding site breast cancer |
| url | http://jams.arakmu.ac.ir/article-1-6111-en.html |
| work_keys_str_mv | AT fatemehtavakoli theassociationofgeneticvariantrs8506ctatmir526bbindingsiteinthelincrnanr024015exonwithbreastcancerrisk AT somayehreiisi theassociationofgeneticvariantrs8506ctatmir526bbindingsiteinthelincrnanr024015exonwithbreastcancerrisk AT fatemehtavakoli associationofgeneticvariantrs8506ctatmir526bbindingsiteinthelincrnanr024015exonwithbreastcancerrisk AT somayehreiisi associationofgeneticvariantrs8506ctatmir526bbindingsiteinthelincrnanr024015exonwithbreastcancerrisk |