Detected Length Polymorphism Shows the Same Characteristics for AFLP and ISSR when Amaranth Gamma-Radiation Mutants are Evaluated
The information characteristics of AFLP and ISSR techniques were compared in the analysis of length polymorphism of gamma-treated mutant lines of Amaranthus cruentus and Amaranthus hypochondriacus x Amaranthus hybridus. Both of the techniques are widely used in the manner of universal marker system...
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| Format: | Article |
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Agroprint Timisoara
2023-09-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
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| Online Access: | https://spasb.ro/index.php/public_html/article/view/844 |
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| author | Jana Žiarovská Ján Gažo Katarína Ražná Mária Labajová Matúš Kyseľ Veronika Lancíková Veronika Štefúnová Andrea Hricová |
| author_facet | Jana Žiarovská Ján Gažo Katarína Ražná Mária Labajová Matúš Kyseľ Veronika Lancíková Veronika Štefúnová Andrea Hricová |
| author_sort | Jana Žiarovská |
| collection | DOAJ |
| description | The information characteristics of AFLP and ISSR techniques were compared in the analysis of length polymorphism of gamma-treated mutant lines of Amaranthus cruentus and Amaranthus hypochondriacus x Amaranthus hybridus. Both of the techniques are widely used in the manner of universal marker system suitable for the analysis of any plant species, but differ in the time needed for the analyse and costs. Binary data matrices were used for statistical analyses. Cluster analysis was performed in UPGMA method using Euclidean distance to construct dendrograms and principal component analyses was used for generated data, too. Comparison of techniques was made on the graphs obtained from result of PCA and dendrograms that were obtained from results of cluster analysis. Matrix comparisons of Mantel test, for the correspondence of the similarity matrices was performed for the null hypothesis that there is no association between similarity matrices. In the analyzed set used in this study, ISSR primers produced 59% polymorphic bands, while AFLP primers produced 48% polymorphic bands and both of techniques were similar in their obtained information characteristics. |
| first_indexed | 2024-04-24T11:24:00Z |
| format | Article |
| id | doaj.art-db265dc783b64055a55d92c159b1ca54 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:24:00Z |
| publishDate | 2023-09-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-db265dc783b64055a55d92c159b1ca542024-04-11T03:54:56ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-09-015225656844Detected Length Polymorphism Shows the Same Characteristics for AFLP and ISSR when Amaranth Gamma-Radiation Mutants are EvaluatedJana Žiarovská0Ján Gažo1Katarína Ražná2Mária Labajová3Matúš Kyseľ4Veronika Lancíková5Veronika Štefúnová6Andrea Hricová7SUA in Nitra, Department of Plant Genetics and Breeding, Tr. A. Hlinku 2, 94976, Nitra, Slovak RepublicSUA in Nitra, Department of Plant Genetics and Breeding, Tr. A. Hlinku 2, 94976, Nitra, Slovak RepublicSUA in Nitra, Department of Plant Genetics and Breeding, Tr. A. Hlinku 2, 94976, Nitra, Slovak RepublicSUA in Nitra, Department of Plant Genetics and Breeding, Tr. A. Hlinku 2, 94976, Nitra, Slovak RepublicSUA in Nitra, Department of Plant Genetics and Breeding, Tr. A. Hlinku 2, 94976, Nitra, Slovak RepublicInstitute of Plan Genetics and Biotechnology, Plant Science and Biodiversity Center, SAS, Akademická 2, 950 07 Nitra, Slovak RepublicSUA in Nitra, Department of Plant Genetics and Breeding, Tr. A. Hlinku 2, 94976, Nitra, Slovak RepublicInstitute of Plan Genetics and Biotechnology, Plant Science and Biodiversity Center, SAS, Akademická 2, 950 07 Nitra, Slovak RepublicThe information characteristics of AFLP and ISSR techniques were compared in the analysis of length polymorphism of gamma-treated mutant lines of Amaranthus cruentus and Amaranthus hypochondriacus x Amaranthus hybridus. Both of the techniques are widely used in the manner of universal marker system suitable for the analysis of any plant species, but differ in the time needed for the analyse and costs. Binary data matrices were used for statistical analyses. Cluster analysis was performed in UPGMA method using Euclidean distance to construct dendrograms and principal component analyses was used for generated data, too. Comparison of techniques was made on the graphs obtained from result of PCA and dendrograms that were obtained from results of cluster analysis. Matrix comparisons of Mantel test, for the correspondence of the similarity matrices was performed for the null hypothesis that there is no association between similarity matrices. In the analyzed set used in this study, ISSR primers produced 59% polymorphic bands, while AFLP primers produced 48% polymorphic bands and both of techniques were similar in their obtained information characteristics.https://spasb.ro/index.php/public_html/article/view/844aflpamaranthcomparisonissr |
| spellingShingle | Jana Žiarovská Ján Gažo Katarína Ražná Mária Labajová Matúš Kyseľ Veronika Lancíková Veronika Štefúnová Andrea Hricová Detected Length Polymorphism Shows the Same Characteristics for AFLP and ISSR when Amaranth Gamma-Radiation Mutants are Evaluated Scientific Papers Animal Science and Biotechnologies aflp amaranth comparison issr |
| title | Detected Length Polymorphism Shows the Same Characteristics for AFLP and ISSR when Amaranth Gamma-Radiation Mutants are Evaluated |
| title_full | Detected Length Polymorphism Shows the Same Characteristics for AFLP and ISSR when Amaranth Gamma-Radiation Mutants are Evaluated |
| title_fullStr | Detected Length Polymorphism Shows the Same Characteristics for AFLP and ISSR when Amaranth Gamma-Radiation Mutants are Evaluated |
| title_full_unstemmed | Detected Length Polymorphism Shows the Same Characteristics for AFLP and ISSR when Amaranth Gamma-Radiation Mutants are Evaluated |
| title_short | Detected Length Polymorphism Shows the Same Characteristics for AFLP and ISSR when Amaranth Gamma-Radiation Mutants are Evaluated |
| title_sort | detected length polymorphism shows the same characteristics for aflp and issr when amaranth gamma radiation mutants are evaluated |
| topic | aflp amaranth comparison issr |
| url | https://spasb.ro/index.php/public_html/article/view/844 |
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