Odorant Receptor PxylOR11 Mediates Repellency of Plutella xylostella to Aromatic Volatiles
Insects can use plant volatiles to guide certain behaviors, such as courtship, mating, host positioning, and habitat selection. Plutella xylostella is a global agricultural pest and has always been closely studied, but relatively few studies assess the molecular mechanism of P. xylostella exposed to...
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| Format: | Article |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2022.938555/full |
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| author | Yipeng Liu Yipeng Liu Sai Zhang Sai Zhang Yang Liu Guirong Wang Guirong Wang |
| author_facet | Yipeng Liu Yipeng Liu Sai Zhang Sai Zhang Yang Liu Guirong Wang Guirong Wang |
| author_sort | Yipeng Liu |
| collection | DOAJ |
| description | Insects can use plant volatiles to guide certain behaviors, such as courtship, mating, host positioning, and habitat selection. Plutella xylostella is a global agricultural pest and has always been closely studied, but relatively few studies assess the molecular mechanism of P. xylostella exposed to plant volatiles. In this study, we analyzed the role of the odorant receptor PxylOR11 when P. xylostella is exposed to plant volatiles. Our analysis of tissue expression demonstrated that PxylOR11 is expressed in the antennae and that expression levels in female moths were significantly higher than in male moths. Functional analyses using the Xenopus oocyte expression system demonstrated that PxylOR11 was tuned to three aromatic compounds: benzyl alcohol, salicylaldehyde, and phenylacetaldehyde. Electroantennogram analyses revealed that these three aromatic compounds can induce electrophysiological responses in the antennae of P. xylostella, and that the electroantennograms response value of female moths was significantly higher than that of male moths. Dual-choice bioassays demonstrated that the three aromatic compounds have a repellent effect on female P. xylostella. These results suggest that PxylOR11 has a role in mediating the repellent effect of aromatic volatiles on P. xylostella and can be used as a potential target to design novel olfactory regulators controlling P. xylostella. |
| first_indexed | 2024-04-13T05:52:38Z |
| format | Article |
| id | doaj.art-db265f5ee7844a0fb87ecbdca796d420 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-13T05:52:38Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-db265f5ee7844a0fb87ecbdca796d4202022-12-22T02:59:43ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2022-07-011310.3389/fphys.2022.938555938555Odorant Receptor PxylOR11 Mediates Repellency of Plutella xylostella to Aromatic VolatilesYipeng Liu0Yipeng Liu1Sai Zhang2Sai Zhang3Yang Liu4Guirong Wang5Guirong Wang6Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaShenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaShenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaInsects can use plant volatiles to guide certain behaviors, such as courtship, mating, host positioning, and habitat selection. Plutella xylostella is a global agricultural pest and has always been closely studied, but relatively few studies assess the molecular mechanism of P. xylostella exposed to plant volatiles. In this study, we analyzed the role of the odorant receptor PxylOR11 when P. xylostella is exposed to plant volatiles. Our analysis of tissue expression demonstrated that PxylOR11 is expressed in the antennae and that expression levels in female moths were significantly higher than in male moths. Functional analyses using the Xenopus oocyte expression system demonstrated that PxylOR11 was tuned to three aromatic compounds: benzyl alcohol, salicylaldehyde, and phenylacetaldehyde. Electroantennogram analyses revealed that these three aromatic compounds can induce electrophysiological responses in the antennae of P. xylostella, and that the electroantennograms response value of female moths was significantly higher than that of male moths. Dual-choice bioassays demonstrated that the three aromatic compounds have a repellent effect on female P. xylostella. These results suggest that PxylOR11 has a role in mediating the repellent effect of aromatic volatiles on P. xylostella and can be used as a potential target to design novel olfactory regulators controlling P. xylostella.https://www.frontiersin.org/articles/10.3389/fphys.2022.938555/fullPlutella xylostellaodorant receptortwo-electrode voltage clamp recordingsbehavioral responserepellent |
| spellingShingle | Yipeng Liu Yipeng Liu Sai Zhang Sai Zhang Yang Liu Guirong Wang Guirong Wang Odorant Receptor PxylOR11 Mediates Repellency of Plutella xylostella to Aromatic Volatiles Frontiers in Physiology Plutella xylostella odorant receptor two-electrode voltage clamp recordings behavioral response repellent |
| title | Odorant Receptor PxylOR11 Mediates Repellency of Plutella xylostella to Aromatic Volatiles |
| title_full | Odorant Receptor PxylOR11 Mediates Repellency of Plutella xylostella to Aromatic Volatiles |
| title_fullStr | Odorant Receptor PxylOR11 Mediates Repellency of Plutella xylostella to Aromatic Volatiles |
| title_full_unstemmed | Odorant Receptor PxylOR11 Mediates Repellency of Plutella xylostella to Aromatic Volatiles |
| title_short | Odorant Receptor PxylOR11 Mediates Repellency of Plutella xylostella to Aromatic Volatiles |
| title_sort | odorant receptor pxylor11 mediates repellency of plutella xylostella to aromatic volatiles |
| topic | Plutella xylostella odorant receptor two-electrode voltage clamp recordings behavioral response repellent |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2022.938555/full |
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