The Nimrod transmembrane receptor Eater is required for hemocyte attachment to the sessile compartment in Drosophila melanogaster
Eater is an EGF-like repeat transmembrane receptor of the Nimrod family and is expressed in Drosophila hemocytes. Eater was initially identified for its role in phagocytosis of both Gram-positive and Gram-negative bacteria. We have deleted eater and show that it appears to be required for efficient...
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2015-02-01
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| Series: | Biology Open |
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| Online Access: | http://bio.biologists.org/content/4/3/355 |
| _version_ | 1818436887246798848 |
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| author | Andrew J. Bretscher Viktor Honti Olivier Binggeli Olivier Burri Mickael Poidevin Éva Kurucz János Zsámboki István Andó Bruno Lemaitre |
| author_facet | Andrew J. Bretscher Viktor Honti Olivier Binggeli Olivier Burri Mickael Poidevin Éva Kurucz János Zsámboki István Andó Bruno Lemaitre |
| author_sort | Andrew J. Bretscher |
| collection | DOAJ |
| description | Eater is an EGF-like repeat transmembrane receptor of the Nimrod family and is expressed in Drosophila hemocytes. Eater was initially identified for its role in phagocytosis of both Gram-positive and Gram-negative bacteria. We have deleted eater and show that it appears to be required for efficient phagocytosis of Gram-positive but not Gram-negative bacteria. However, the most striking phenotype of eater deficient larvae is the near absence of sessile hemocytes, both plasmatocyte and crystal cell types. The eater deletion is the first loss of function mutation identified that causes absence of the sessile hemocyte state. Our study shows that Eater is required cell-autonomously in plasmatocytes for sessility. However, the presence of crystal cells in the sessile compartment requires Eater in plasmatocytes. We also show that eater deficient hemocytes exhibit a cell adhesion defect. Collectively, our data uncovers a new requirement of Eater in enabling hemocyte attachment at the sessile compartment and points to a possible role of Nimrod family members in hemocyte adhesion. |
| first_indexed | 2024-12-14T17:15:55Z |
| format | Article |
| id | doaj.art-db26b8d3d086487b906d3ea29c89ebb9 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-14T17:15:55Z |
| publishDate | 2015-02-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-db26b8d3d086487b906d3ea29c89ebb92022-12-21T22:53:26ZengThe Company of BiologistsBiology Open2046-63902015-02-014335536310.1242/bio.201410595201410595The Nimrod transmembrane receptor Eater is required for hemocyte attachment to the sessile compartment in Drosophila melanogasterAndrew J. Bretscher0Viktor Honti1Olivier Binggeli2Olivier Burri3Mickael Poidevin4Éva Kurucz5János Zsámboki6István Andó7Bruno Lemaitre8 Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 19, 1015 Lausanne, Switzerland Institute of Genetics Biological Research Centre of the Hungarian Academy of Sciences, P.O. Box 521, Szeged H-6701, Hungary Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 19, 1015 Lausanne, Switzerland Bioimaging and Optics Platform, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 19, 1015 Lausanne, Switzerland Centre de Génétique Moléculaire, CNRS/Université Pierre et Marie Curie, 91198 Gif-sur-Yvette, France Institute of Genetics Biological Research Centre of the Hungarian Academy of Sciences, P.O. Box 521, Szeged H-6701, Hungary Institute of Genetics Biological Research Centre of the Hungarian Academy of Sciences, P.O. Box 521, Szeged H-6701, Hungary Institute of Genetics Biological Research Centre of the Hungarian Academy of Sciences, P.O. Box 521, Szeged H-6701, Hungary Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 19, 1015 Lausanne, Switzerland Eater is an EGF-like repeat transmembrane receptor of the Nimrod family and is expressed in Drosophila hemocytes. Eater was initially identified for its role in phagocytosis of both Gram-positive and Gram-negative bacteria. We have deleted eater and show that it appears to be required for efficient phagocytosis of Gram-positive but not Gram-negative bacteria. However, the most striking phenotype of eater deficient larvae is the near absence of sessile hemocytes, both plasmatocyte and crystal cell types. The eater deletion is the first loss of function mutation identified that causes absence of the sessile hemocyte state. Our study shows that Eater is required cell-autonomously in plasmatocytes for sessility. However, the presence of crystal cells in the sessile compartment requires Eater in plasmatocytes. We also show that eater deficient hemocytes exhibit a cell adhesion defect. Collectively, our data uncovers a new requirement of Eater in enabling hemocyte attachment at the sessile compartment and points to a possible role of Nimrod family members in hemocyte adhesion.http://bio.biologists.org/content/4/3/355EGF-likeEaterHemocyteReceptorSessile |
| spellingShingle | Andrew J. Bretscher Viktor Honti Olivier Binggeli Olivier Burri Mickael Poidevin Éva Kurucz János Zsámboki István Andó Bruno Lemaitre The Nimrod transmembrane receptor Eater is required for hemocyte attachment to the sessile compartment in Drosophila melanogaster Biology Open EGF-like Eater Hemocyte Receptor Sessile |
| title | The Nimrod transmembrane receptor Eater is required for hemocyte attachment to the sessile compartment in Drosophila melanogaster |
| title_full | The Nimrod transmembrane receptor Eater is required for hemocyte attachment to the sessile compartment in Drosophila melanogaster |
| title_fullStr | The Nimrod transmembrane receptor Eater is required for hemocyte attachment to the sessile compartment in Drosophila melanogaster |
| title_full_unstemmed | The Nimrod transmembrane receptor Eater is required for hemocyte attachment to the sessile compartment in Drosophila melanogaster |
| title_short | The Nimrod transmembrane receptor Eater is required for hemocyte attachment to the sessile compartment in Drosophila melanogaster |
| title_sort | nimrod transmembrane receptor eater is required for hemocyte attachment to the sessile compartment in drosophila melanogaster |
| topic | EGF-like Eater Hemocyte Receptor Sessile |
| url | http://bio.biologists.org/content/4/3/355 |
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