<i>Ginkgo biloba</i> Prevents Oxidative Stress-Induced Apoptosis Blocking p53 Activation in Neuroblastoma Cells
Oxidative stress has been associated to neuronal cell loss in neurodegenerative diseases. Neurons are post-mitotic cells that are very sensitive to oxidative stress—especially considering their limited capacity to be replaced. Therefore, reduction of oxidative stress, and inhibiting apopto...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-03-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/9/4/279 |
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| author | Francesco Di Meo Rossana Cuciniello Sabrina Margarucci Paolo Bergamo Orsolina Petillo Gianfranco Peluso Stefania Filosa Stefania Crispi |
| author_facet | Francesco Di Meo Rossana Cuciniello Sabrina Margarucci Paolo Bergamo Orsolina Petillo Gianfranco Peluso Stefania Filosa Stefania Crispi |
| author_sort | Francesco Di Meo |
| collection | DOAJ |
| description | Oxidative stress has been associated to neuronal cell loss in neurodegenerative diseases. Neurons are post-mitotic cells that are very sensitive to oxidative stress—especially considering their limited capacity to be replaced. Therefore, reduction of oxidative stress, and inhibiting apoptosis, will potentially prevent neurodegeneration. In this study, we investigated the neuroprotective effect of Ginkgo biloba extract (EGb 761) against H<sub>2</sub>O<sub>2</sub> induced apoptosis in SK-N-BE neuroblastoma cells. We analysed the molecular signalling pathway involved in the apoptotic cell death. H<sub>2</sub>O<sub>2</sub> induced an increased acetylation of p53 lysine 382, a reduction in mitochondrial membrane potential, an increased BAX/Bcl-2 ratio and consequently increased Poly (ADP-ribose) polymerase (PARP) cleavage. All these effects were blocked by EGb 761 treatment. Thus, EGb 761, acting as intracellular antioxidant, protects neuroblastoma cells against activation of p53 mediated pathway and intrinsic mitochondrial apoptosis. Our results suggest that EGb 761, protecting against oxidative-stress induced apoptotic cell death, could potentially be used as nutraceutical for the prevention and treatment of neurodegenerative diseases. |
| first_indexed | 2024-03-12T09:52:46Z |
| format | Article |
| id | doaj.art-db2704c324cf4e55b3bb307b2649176d |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T09:52:46Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-db2704c324cf4e55b3bb307b2649176d2023-09-02T12:26:28ZengMDPI AGAntioxidants2076-39212020-03-019427910.3390/antiox9040279antiox9040279<i>Ginkgo biloba</i> Prevents Oxidative Stress-Induced Apoptosis Blocking p53 Activation in Neuroblastoma CellsFrancesco Di Meo0Rossana Cuciniello1Sabrina Margarucci2Paolo Bergamo3Orsolina Petillo4Gianfranco Peluso5Stefania Filosa6Stefania Crispi7Institute of Biosciences and BioResources-UOS Naples CNR, Via P. Castellino, 111- 80131 Naples, ItalyInstitute of Biosciences and BioResources-UOS Naples CNR, Via P. Castellino, 111- 80131 Naples, ItalyInstitute on Terrestrial Ecosystems (IRET) CNR, Via P. Castellino 111, 80131 Naples, ItalyInstitute of Food Science CNR, Via Roma, 64, 83100 Avellino, ItalyInstitute on Terrestrial Ecosystems (IRET) CNR, Via P. Castellino 111, 80131 Naples, ItalyInstitute on Terrestrial Ecosystems (IRET) CNR, Via P. Castellino 111, 80131 Naples, ItalyInstitute of Biosciences and BioResources-UOS Naples CNR, Via P. Castellino, 111- 80131 Naples, ItalyInstitute of Biosciences and BioResources-UOS Naples CNR, Via P. Castellino, 111- 80131 Naples, ItalyOxidative stress has been associated to neuronal cell loss in neurodegenerative diseases. Neurons are post-mitotic cells that are very sensitive to oxidative stress—especially considering their limited capacity to be replaced. Therefore, reduction of oxidative stress, and inhibiting apoptosis, will potentially prevent neurodegeneration. In this study, we investigated the neuroprotective effect of Ginkgo biloba extract (EGb 761) against H<sub>2</sub>O<sub>2</sub> induced apoptosis in SK-N-BE neuroblastoma cells. We analysed the molecular signalling pathway involved in the apoptotic cell death. H<sub>2</sub>O<sub>2</sub> induced an increased acetylation of p53 lysine 382, a reduction in mitochondrial membrane potential, an increased BAX/Bcl-2 ratio and consequently increased Poly (ADP-ribose) polymerase (PARP) cleavage. All these effects were blocked by EGb 761 treatment. Thus, EGb 761, acting as intracellular antioxidant, protects neuroblastoma cells against activation of p53 mediated pathway and intrinsic mitochondrial apoptosis. Our results suggest that EGb 761, protecting against oxidative-stress induced apoptotic cell death, could potentially be used as nutraceutical for the prevention and treatment of neurodegenerative diseases.https://www.mdpi.com/2076-3921/9/4/279ginkgo bilobaoxidative stressp53apoptosisneuroprotectionneuroblastoma cells |
| spellingShingle | Francesco Di Meo Rossana Cuciniello Sabrina Margarucci Paolo Bergamo Orsolina Petillo Gianfranco Peluso Stefania Filosa Stefania Crispi <i>Ginkgo biloba</i> Prevents Oxidative Stress-Induced Apoptosis Blocking p53 Activation in Neuroblastoma Cells Antioxidants ginkgo biloba oxidative stress p53 apoptosis neuroprotection neuroblastoma cells |
| title | <i>Ginkgo biloba</i> Prevents Oxidative Stress-Induced Apoptosis Blocking p53 Activation in Neuroblastoma Cells |
| title_full | <i>Ginkgo biloba</i> Prevents Oxidative Stress-Induced Apoptosis Blocking p53 Activation in Neuroblastoma Cells |
| title_fullStr | <i>Ginkgo biloba</i> Prevents Oxidative Stress-Induced Apoptosis Blocking p53 Activation in Neuroblastoma Cells |
| title_full_unstemmed | <i>Ginkgo biloba</i> Prevents Oxidative Stress-Induced Apoptosis Blocking p53 Activation in Neuroblastoma Cells |
| title_short | <i>Ginkgo biloba</i> Prevents Oxidative Stress-Induced Apoptosis Blocking p53 Activation in Neuroblastoma Cells |
| title_sort | i ginkgo biloba i prevents oxidative stress induced apoptosis blocking p53 activation in neuroblastoma cells |
| topic | ginkgo biloba oxidative stress p53 apoptosis neuroprotection neuroblastoma cells |
| url | https://www.mdpi.com/2076-3921/9/4/279 |
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