The Kinase USK1 Regulates Cellulase Gene Expression and Secondary Metabolite Biosynthesis in Trichoderma reesei
The complex environment of fungi requires a delicate balance between the efforts to acquire nutrition, to reproduce, and to fend off competitors. In Trichoderma reesei, an interrelationship between regulation of enzyme gene expression and secondary metabolism was shown. In this study, we investigate...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.00974/full |
| _version_ | 1818337909171814400 |
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| author | Sabrina Beier Wolfgang Hinterdobler Alberto Alonso Monroy Hoda Bazafkan Monika Schmoll |
| author_facet | Sabrina Beier Wolfgang Hinterdobler Alberto Alonso Monroy Hoda Bazafkan Monika Schmoll |
| author_sort | Sabrina Beier |
| collection | DOAJ |
| description | The complex environment of fungi requires a delicate balance between the efforts to acquire nutrition, to reproduce, and to fend off competitors. In Trichoderma reesei, an interrelationship between regulation of enzyme gene expression and secondary metabolism was shown. In this study, we investigated the physiological relevance of the unique YPK1-type kinase USK1 of T. reesei. Usk1 is located in the vicinity of the SOR cluster and is involved in regulation of several genes from this secondary metabolite cluster as well as dihydrotrichotetronine and other secondary metabolites. Moreover, USK1 is required for biosynthesis of normal levels of secondary metabolites in liquid culture. USK1 positively influences cellulase gene regulation, secreted cellulase activity, and biomass formation upon growth in constant darkness on cellulose. Positive effects of USK1 on transcript abundance of the regulator of secondary metabolism, vel1, and the carbon catabolite repressor gene cre1 are in agreement with these functions. In summary, we found that with USK1, T. reesei comprises a unique kinase that adds an additional layer of regulation to the connection of secondary metabolism and enzyme production in fungi. |
| first_indexed | 2024-12-13T15:02:42Z |
| format | Article |
| id | doaj.art-db2807c7d2a042c1ac45891cbdb60f3a |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T15:02:42Z |
| publishDate | 2020-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-db2807c7d2a042c1ac45891cbdb60f3a2022-12-21T23:41:05ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-05-011110.3389/fmicb.2020.00974512422The Kinase USK1 Regulates Cellulase Gene Expression and Secondary Metabolite Biosynthesis in Trichoderma reeseiSabrina BeierWolfgang HinterdoblerAlberto Alonso MonroyHoda BazafkanMonika SchmollThe complex environment of fungi requires a delicate balance between the efforts to acquire nutrition, to reproduce, and to fend off competitors. In Trichoderma reesei, an interrelationship between regulation of enzyme gene expression and secondary metabolism was shown. In this study, we investigated the physiological relevance of the unique YPK1-type kinase USK1 of T. reesei. Usk1 is located in the vicinity of the SOR cluster and is involved in regulation of several genes from this secondary metabolite cluster as well as dihydrotrichotetronine and other secondary metabolites. Moreover, USK1 is required for biosynthesis of normal levels of secondary metabolites in liquid culture. USK1 positively influences cellulase gene regulation, secreted cellulase activity, and biomass formation upon growth in constant darkness on cellulose. Positive effects of USK1 on transcript abundance of the regulator of secondary metabolism, vel1, and the carbon catabolite repressor gene cre1 are in agreement with these functions. In summary, we found that with USK1, T. reesei comprises a unique kinase that adds an additional layer of regulation to the connection of secondary metabolism and enzyme production in fungi.https://www.frontiersin.org/article/10.3389/fmicb.2020.00974/fullTrichoderma reeseiHypocrea jecorinasorbicillindihydrotrichotetroninsecondary metabolismplant cell wall degradation |
| spellingShingle | Sabrina Beier Wolfgang Hinterdobler Alberto Alonso Monroy Hoda Bazafkan Monika Schmoll The Kinase USK1 Regulates Cellulase Gene Expression and Secondary Metabolite Biosynthesis in Trichoderma reesei Frontiers in Microbiology Trichoderma reesei Hypocrea jecorina sorbicillin dihydrotrichotetronin secondary metabolism plant cell wall degradation |
| title | The Kinase USK1 Regulates Cellulase Gene Expression and Secondary Metabolite Biosynthesis in Trichoderma reesei |
| title_full | The Kinase USK1 Regulates Cellulase Gene Expression and Secondary Metabolite Biosynthesis in Trichoderma reesei |
| title_fullStr | The Kinase USK1 Regulates Cellulase Gene Expression and Secondary Metabolite Biosynthesis in Trichoderma reesei |
| title_full_unstemmed | The Kinase USK1 Regulates Cellulase Gene Expression and Secondary Metabolite Biosynthesis in Trichoderma reesei |
| title_short | The Kinase USK1 Regulates Cellulase Gene Expression and Secondary Metabolite Biosynthesis in Trichoderma reesei |
| title_sort | kinase usk1 regulates cellulase gene expression and secondary metabolite biosynthesis in trichoderma reesei |
| topic | Trichoderma reesei Hypocrea jecorina sorbicillin dihydrotrichotetronin secondary metabolism plant cell wall degradation |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.00974/full |
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