Pro-Atherogenic and Pro-Oxidant Diets Influence Semen and Blood Traits of Rabbit Bucks
Many dietary factors can affect sperm traits. We compared the effect of diets rich in pro-oxidant (flaxseed oil) and pro-atherogenic (coconut oil) substances without added antioxidants on semen traits, using the rabbit as an animal model. Thirty rabbit bucks (8 months old) were fed three diets for 1...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/10/1880 |
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| author | Simona Mattioli Elisa Angelucci Alessandro Dal Bosco Cinzia Signorini Lakamy Sylla Luigia Bosa Giulia Collodel Thierry Durand Jean-Marie Galano Camille Oger Cesare Castellini |
| author_facet | Simona Mattioli Elisa Angelucci Alessandro Dal Bosco Cinzia Signorini Lakamy Sylla Luigia Bosa Giulia Collodel Thierry Durand Jean-Marie Galano Camille Oger Cesare Castellini |
| author_sort | Simona Mattioli |
| collection | DOAJ |
| description | Many dietary factors can affect sperm traits. We compared the effect of diets rich in pro-oxidant (flaxseed oil) and pro-atherogenic (coconut oil) substances without added antioxidants on semen traits, using the rabbit as an animal model. Thirty rabbit bucks (8 months old) were fed three diets for 150 days: CNT (control) a standard diet; HA (high-atherogenic) standard diet + 3% coconut oil, and HO (high-oxidizing) standard diet + 3% flaxseed oil. Semen samples were collected weekly for the evaluation of qualitative traits (kinetics, viability) and the oxidative damage (MDA and cytokines). Blood was collected at the start (T0) and end (T8) of the experimental period for the assessment of the oxidative damage (MDA and isoprostanoids), lipid profile, and testosterone. A worsening of sperm kinetics and viability was recorded in the HA group. Lipid oxidation in seminal plasma, as well as isoprostanoids in blood (F<sub>3</sub>-IsoPs and F<sub>4</sub>-NeuroPs), increased in both the HO and HA groups. A high level of TNF-α, a marker of inflammatory status, was recorded in the seminal plasma of the HA group. The resulting outcomes were mainly attributable to the different fatty acid profiles (SFA vs. PUFA) of the diets, which modulated an inflammatory/oxidative response. |
| first_indexed | 2024-03-10T21:29:49Z |
| format | Article |
| id | doaj.art-db29b8055c324d3083409eb0a36b814c |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T21:29:49Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-db29b8055c324d3083409eb0a36b814c2023-11-19T15:28:29ZengMDPI AGAntioxidants2076-39212023-10-011210188010.3390/antiox12101880Pro-Atherogenic and Pro-Oxidant Diets Influence Semen and Blood Traits of Rabbit BucksSimona Mattioli0Elisa Angelucci1Alessandro Dal Bosco2Cinzia Signorini3Lakamy Sylla4Luigia Bosa5Giulia Collodel6Thierry Durand7Jean-Marie Galano8Camille Oger9Cesare Castellini10Department of Agricultural, Environmental and Food Science, University of Perugia, Borgo XX Giugno 74, 06124 Perugia, ItalyDepartment of Agricultural, Environmental and Food Science, University of Perugia, Borgo XX Giugno 74, 06124 Perugia, ItalyDepartment of Agricultural, Environmental and Food Science, University of Perugia, Borgo XX Giugno 74, 06124 Perugia, ItalyDepartment of Molecular and Developmental Medicine, University of Siena, Policlinico Santa Maria alle Scotte, Viale Bracci, 14, 53100 Siena, ItalyDepartment of Veterinary Medicine, University of Perugia, Via S. Costanzo 4, 06126 Perugia, ItalyDepartment of Agricultural, Environmental and Food Science, University of Perugia, Borgo XX Giugno 74, 06124 Perugia, ItalyDepartment of Molecular and Developmental Medicine, University of Siena, Policlinico Santa Maria alle Scotte, Viale Bracci, 14, 53100 Siena, ItalyInstitut des Biomolécules Max Mousseron (IBMM), UMR, CNRS, Université de Montpellier, ENSCM, 5247 Montpellier, FranceInstitut des Biomolécules Max Mousseron (IBMM), UMR, CNRS, Université de Montpellier, ENSCM, 5247 Montpellier, FranceInstitut des Biomolécules Max Mousseron (IBMM), UMR, CNRS, Université de Montpellier, ENSCM, 5247 Montpellier, FranceDepartment of Agricultural, Environmental and Food Science, University of Perugia, Borgo XX Giugno 74, 06124 Perugia, ItalyMany dietary factors can affect sperm traits. We compared the effect of diets rich in pro-oxidant (flaxseed oil) and pro-atherogenic (coconut oil) substances without added antioxidants on semen traits, using the rabbit as an animal model. Thirty rabbit bucks (8 months old) were fed three diets for 150 days: CNT (control) a standard diet; HA (high-atherogenic) standard diet + 3% coconut oil, and HO (high-oxidizing) standard diet + 3% flaxseed oil. Semen samples were collected weekly for the evaluation of qualitative traits (kinetics, viability) and the oxidative damage (MDA and cytokines). Blood was collected at the start (T0) and end (T8) of the experimental period for the assessment of the oxidative damage (MDA and isoprostanoids), lipid profile, and testosterone. A worsening of sperm kinetics and viability was recorded in the HA group. Lipid oxidation in seminal plasma, as well as isoprostanoids in blood (F<sub>3</sub>-IsoPs and F<sub>4</sub>-NeuroPs), increased in both the HO and HA groups. A high level of TNF-α, a marker of inflammatory status, was recorded in the seminal plasma of the HA group. The resulting outcomes were mainly attributable to the different fatty acid profiles (SFA vs. PUFA) of the diets, which modulated an inflammatory/oxidative response.https://www.mdpi.com/2076-3921/12/10/1880saturated fatty acidspolyunsaturated fatty acidssemenrabbitoxidationinflammation |
| spellingShingle | Simona Mattioli Elisa Angelucci Alessandro Dal Bosco Cinzia Signorini Lakamy Sylla Luigia Bosa Giulia Collodel Thierry Durand Jean-Marie Galano Camille Oger Cesare Castellini Pro-Atherogenic and Pro-Oxidant Diets Influence Semen and Blood Traits of Rabbit Bucks Antioxidants saturated fatty acids polyunsaturated fatty acids semen rabbit oxidation inflammation |
| title | Pro-Atherogenic and Pro-Oxidant Diets Influence Semen and Blood Traits of Rabbit Bucks |
| title_full | Pro-Atherogenic and Pro-Oxidant Diets Influence Semen and Blood Traits of Rabbit Bucks |
| title_fullStr | Pro-Atherogenic and Pro-Oxidant Diets Influence Semen and Blood Traits of Rabbit Bucks |
| title_full_unstemmed | Pro-Atherogenic and Pro-Oxidant Diets Influence Semen and Blood Traits of Rabbit Bucks |
| title_short | Pro-Atherogenic and Pro-Oxidant Diets Influence Semen and Blood Traits of Rabbit Bucks |
| title_sort | pro atherogenic and pro oxidant diets influence semen and blood traits of rabbit bucks |
| topic | saturated fatty acids polyunsaturated fatty acids semen rabbit oxidation inflammation |
| url | https://www.mdpi.com/2076-3921/12/10/1880 |
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