Hfq Regulates Efflux Pump Expression and Purine Metabolic Pathway to Increase Trimethoprim Resistance in Aeromonas veronii
Aeromonas veronii (A. veronii) is a zoonotic pathogen. It causes clinically a variety of diseases such as dysentery, bacteremia, and meningitis, and brings huge losses to aquaculture. A. veronii has been documented as a multiple antibiotic resistant bacterium. Hfq (host factor for RNA bacteriophage...
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.742114/full |
| _version_ | 1818936146083708928 |
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| author | Dan Wang Dan Wang Hong Li Xiang Ma Yanqiong Tang Hongqian Tang Dongyi Huang Min Lin Zhu Liu |
| author_facet | Dan Wang Dan Wang Hong Li Xiang Ma Yanqiong Tang Hongqian Tang Dongyi Huang Min Lin Zhu Liu |
| author_sort | Dan Wang |
| collection | DOAJ |
| description | Aeromonas veronii (A. veronii) is a zoonotic pathogen. It causes clinically a variety of diseases such as dysentery, bacteremia, and meningitis, and brings huge losses to aquaculture. A. veronii has been documented as a multiple antibiotic resistant bacterium. Hfq (host factor for RNA bacteriophage Qβ replication) participates in the regulations of the virulence, adhesion, and nitrogen fixation, effecting on the growth, metabolism synthesis and stress resistance in bacteria. The deletion of hfq gene in A. veronii showed more sensitivity to trimethoprim, accompanying by the upregulations of purine metabolic genes and downregulations of efflux pump genes by transcriptomic data analysis. Coherently, the complementation of efflux pump-related genes acrA and acrB recovered the trimethoprim resistance in Δhfq. Besides, the accumulations of adenosine and guanosine were increased in Δhfq in metabonomic data. The strain Δhfq conferred more sensitive to trimethoprim after appending 1 mM guanosine to M9 medium, while wild type was not altered. These results demonstrated that Hfq mediated trimethoprim resistance by elevating efflux pump expression and degrading adenosine, and guanosine metabolites. Collectively, Hfq is a potential target to tackle trimethoprim resistance in A. veronii infection. |
| first_indexed | 2024-12-20T05:31:25Z |
| format | Article |
| id | doaj.art-db2dcd9052884620832db92727074e02 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-20T05:31:25Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-db2dcd9052884620832db92727074e022022-12-21T19:51:44ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-11-011210.3389/fmicb.2021.742114742114Hfq Regulates Efflux Pump Expression and Purine Metabolic Pathway to Increase Trimethoprim Resistance in Aeromonas veroniiDan Wang0Dan Wang1Hong Li2Xiang Ma3Yanqiong Tang4Hongqian Tang5Dongyi Huang6Min Lin7Zhu Liu8College of Life Sciences, Hainan University, Haikou, ChinaCollege of Tropical Crops Hainan University, Haikou, ChinaCollege of Life Sciences, Hainan University, Haikou, ChinaCollege of Life Sciences, Hainan University, Haikou, ChinaCollege of Life Sciences, Hainan University, Haikou, ChinaCollege of Life Sciences, Hainan University, Haikou, ChinaCollege of Tropical Crops Hainan University, Haikou, ChinaChinese Academy of Agricultural Science, Beijing, ChinaCollege of Life Sciences, Hainan University, Haikou, ChinaAeromonas veronii (A. veronii) is a zoonotic pathogen. It causes clinically a variety of diseases such as dysentery, bacteremia, and meningitis, and brings huge losses to aquaculture. A. veronii has been documented as a multiple antibiotic resistant bacterium. Hfq (host factor for RNA bacteriophage Qβ replication) participates in the regulations of the virulence, adhesion, and nitrogen fixation, effecting on the growth, metabolism synthesis and stress resistance in bacteria. The deletion of hfq gene in A. veronii showed more sensitivity to trimethoprim, accompanying by the upregulations of purine metabolic genes and downregulations of efflux pump genes by transcriptomic data analysis. Coherently, the complementation of efflux pump-related genes acrA and acrB recovered the trimethoprim resistance in Δhfq. Besides, the accumulations of adenosine and guanosine were increased in Δhfq in metabonomic data. The strain Δhfq conferred more sensitive to trimethoprim after appending 1 mM guanosine to M9 medium, while wild type was not altered. These results demonstrated that Hfq mediated trimethoprim resistance by elevating efflux pump expression and degrading adenosine, and guanosine metabolites. Collectively, Hfq is a potential target to tackle trimethoprim resistance in A. veronii infection.https://www.frontiersin.org/articles/10.3389/fmicb.2021.742114/fullAeromonas veroniiHfqtrimethoprimantibiotic resistanceacrA/acrBpurine pathway |
| spellingShingle | Dan Wang Dan Wang Hong Li Xiang Ma Yanqiong Tang Hongqian Tang Dongyi Huang Min Lin Zhu Liu Hfq Regulates Efflux Pump Expression and Purine Metabolic Pathway to Increase Trimethoprim Resistance in Aeromonas veronii Frontiers in Microbiology Aeromonas veronii Hfq trimethoprim antibiotic resistance acrA/acrB purine pathway |
| title | Hfq Regulates Efflux Pump Expression and Purine Metabolic Pathway to Increase Trimethoprim Resistance in Aeromonas veronii |
| title_full | Hfq Regulates Efflux Pump Expression and Purine Metabolic Pathway to Increase Trimethoprim Resistance in Aeromonas veronii |
| title_fullStr | Hfq Regulates Efflux Pump Expression and Purine Metabolic Pathway to Increase Trimethoprim Resistance in Aeromonas veronii |
| title_full_unstemmed | Hfq Regulates Efflux Pump Expression and Purine Metabolic Pathway to Increase Trimethoprim Resistance in Aeromonas veronii |
| title_short | Hfq Regulates Efflux Pump Expression and Purine Metabolic Pathway to Increase Trimethoprim Resistance in Aeromonas veronii |
| title_sort | hfq regulates efflux pump expression and purine metabolic pathway to increase trimethoprim resistance in aeromonas veronii |
| topic | Aeromonas veronii Hfq trimethoprim antibiotic resistance acrA/acrB purine pathway |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.742114/full |
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