Detection of Seasonal Variation in Aloe Polysaccharides Using Carbohydrate Detecting Microarrays
Aloe vera gel is a globally popular natural product used for the treatment of skin conditions. Its useful properties are attributed to the presence of bioactive polysaccharides. Nearly 25% of the 600 species in the genus Aloe are used locally in traditional medicine, indicating that the bioactive co...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2019-05-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.00512/full |
| _version_ | 1818176537665470464 |
|---|---|
| author | Louise Isager Ahl Narjes Al-Husseini Sara Al-Helle Dan Staerk Olwen M. Grace William G. T. Willats Jozef Mravec Bodil Jørgensen Nina Rønsted |
| author_facet | Louise Isager Ahl Narjes Al-Husseini Sara Al-Helle Dan Staerk Olwen M. Grace William G. T. Willats Jozef Mravec Bodil Jørgensen Nina Rønsted |
| author_sort | Louise Isager Ahl |
| collection | DOAJ |
| description | Aloe vera gel is a globally popular natural product used for the treatment of skin conditions. Its useful properties are attributed to the presence of bioactive polysaccharides. Nearly 25% of the 600 species in the genus Aloe are used locally in traditional medicine, indicating that the bioactive components in Aloe vera may be common across the genus Aloe. The complexity of the polysaccharides has hindered development of relevant assays for authentication of Aloe products. Carbohydrate detecting microarrays have recently been suggested as a method for profiling Aloe polysaccharide composition. The aim of this study was to use carbohydrate detecting microarrays to investigate the seasonal variation in the polysaccharide composition of two medicinal and two non-medicinal Aloe species over the course of a year. Microscopy was used to explore where in the cells the bioactive polysaccharides are present and predict their functional role in the cell wall structure. The carbohydrate detecting microarrays analyses showed distinctive differences in the polysaccharide composition between the different species and carbohydrate detecting microarrays therefore has potential as a complementary screening method directly targeting the presence and composition of relevant polysaccharides. The results also show changes in the polysaccharide composition over the year within the investigated species, which may be of importance for commercial growing in optimizing harvest times to obtain higher yield of relevant polysaccharides. |
| first_indexed | 2024-12-11T20:17:46Z |
| format | Article |
| id | doaj.art-db31cfb8fd9e4169850f8fed072442f4 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-11T20:17:46Z |
| publishDate | 2019-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-db31cfb8fd9e4169850f8fed072442f42022-12-22T00:52:09ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-05-011010.3389/fpls.2019.00512440013Detection of Seasonal Variation in Aloe Polysaccharides Using Carbohydrate Detecting MicroarraysLouise Isager Ahl0Narjes Al-Husseini1Sara Al-Helle2Dan Staerk3Olwen M. Grace4William G. T. Willats5Jozef Mravec6Bodil Jørgensen7Nina Rønsted8Natural History Museum of Denmark, University of Copenhagen, Copenhagen, DenmarkNatural History Museum of Denmark, University of Copenhagen, Copenhagen, DenmarkNatural History Museum of Denmark, University of Copenhagen, Copenhagen, DenmarkDepartment of Drug Design and Pharmacology, University of Copenhagen, Copenhagen, DenmarkComparative Plant and Fungal Biology, Royal Botanic Gardens Kew, Richmond, United KingdomSchool of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, United KingdomDepartment of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, DenmarkDepartment of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, DenmarkNatural History Museum of Denmark, University of Copenhagen, Copenhagen, DenmarkAloe vera gel is a globally popular natural product used for the treatment of skin conditions. Its useful properties are attributed to the presence of bioactive polysaccharides. Nearly 25% of the 600 species in the genus Aloe are used locally in traditional medicine, indicating that the bioactive components in Aloe vera may be common across the genus Aloe. The complexity of the polysaccharides has hindered development of relevant assays for authentication of Aloe products. Carbohydrate detecting microarrays have recently been suggested as a method for profiling Aloe polysaccharide composition. The aim of this study was to use carbohydrate detecting microarrays to investigate the seasonal variation in the polysaccharide composition of two medicinal and two non-medicinal Aloe species over the course of a year. Microscopy was used to explore where in the cells the bioactive polysaccharides are present and predict their functional role in the cell wall structure. The carbohydrate detecting microarrays analyses showed distinctive differences in the polysaccharide composition between the different species and carbohydrate detecting microarrays therefore has potential as a complementary screening method directly targeting the presence and composition of relevant polysaccharides. The results also show changes in the polysaccharide composition over the year within the investigated species, which may be of importance for commercial growing in optimizing harvest times to obtain higher yield of relevant polysaccharides.https://www.frontiersin.org/article/10.3389/fpls.2019.00512/fullAloeauthenticationcarbohydrate detecting microarraysplant cell wallspolysaccharidesseasonal variation |
| spellingShingle | Louise Isager Ahl Narjes Al-Husseini Sara Al-Helle Dan Staerk Olwen M. Grace William G. T. Willats Jozef Mravec Bodil Jørgensen Nina Rønsted Detection of Seasonal Variation in Aloe Polysaccharides Using Carbohydrate Detecting Microarrays Frontiers in Plant Science Aloe authentication carbohydrate detecting microarrays plant cell walls polysaccharides seasonal variation |
| title | Detection of Seasonal Variation in Aloe Polysaccharides Using Carbohydrate Detecting Microarrays |
| title_full | Detection of Seasonal Variation in Aloe Polysaccharides Using Carbohydrate Detecting Microarrays |
| title_fullStr | Detection of Seasonal Variation in Aloe Polysaccharides Using Carbohydrate Detecting Microarrays |
| title_full_unstemmed | Detection of Seasonal Variation in Aloe Polysaccharides Using Carbohydrate Detecting Microarrays |
| title_short | Detection of Seasonal Variation in Aloe Polysaccharides Using Carbohydrate Detecting Microarrays |
| title_sort | detection of seasonal variation in aloe polysaccharides using carbohydrate detecting microarrays |
| topic | Aloe authentication carbohydrate detecting microarrays plant cell walls polysaccharides seasonal variation |
| url | https://www.frontiersin.org/article/10.3389/fpls.2019.00512/full |
| work_keys_str_mv | AT louiseisagerahl detectionofseasonalvariationinaloepolysaccharidesusingcarbohydratedetectingmicroarrays AT narjesalhusseini detectionofseasonalvariationinaloepolysaccharidesusingcarbohydratedetectingmicroarrays AT saraalhelle detectionofseasonalvariationinaloepolysaccharidesusingcarbohydratedetectingmicroarrays AT danstaerk detectionofseasonalvariationinaloepolysaccharidesusingcarbohydratedetectingmicroarrays AT olwenmgrace detectionofseasonalvariationinaloepolysaccharidesusingcarbohydratedetectingmicroarrays AT williamgtwillats detectionofseasonalvariationinaloepolysaccharidesusingcarbohydratedetectingmicroarrays AT jozefmravec detectionofseasonalvariationinaloepolysaccharidesusingcarbohydratedetectingmicroarrays AT bodiljørgensen detectionofseasonalvariationinaloepolysaccharidesusingcarbohydratedetectingmicroarrays AT ninarønsted detectionofseasonalvariationinaloepolysaccharidesusingcarbohydratedetectingmicroarrays |