An Ethylene-inhibited NF-YC Transcription Factor RhNF-YC9 Regulates Petal Expansion in Rose
The speed of flower opening is closely related to their ornamental period. Ethylene functions as a negative regulator involved in the regulation of the petal expansion process. In this study, we isolated a NF-YC transcription factor gene, RhNF-YC9, from rose petals. RhNF-YC9 expression was induced a...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2020-11-01
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| Series: | Horticultural Plant Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014120301114 |
| _version_ | 1797203330521366528 |
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| author | Changxi Chen Nisar Hussain Yaru Wang Mingtong Li Lin Liu Meizhu Qin Nan Ma Junping Gao Xiaoming Sun |
| author_facet | Changxi Chen Nisar Hussain Yaru Wang Mingtong Li Lin Liu Meizhu Qin Nan Ma Junping Gao Xiaoming Sun |
| author_sort | Changxi Chen |
| collection | DOAJ |
| description | The speed of flower opening is closely related to their ornamental period. Ethylene functions as a negative regulator involved in the regulation of the petal expansion process. In this study, we isolated a NF-YC transcription factor gene, RhNF-YC9, from rose petals. RhNF-YC9 expression was induced at the early stages of flower opening but was inhibited by ethylene treatment. Silencing RhNF-YC9 decreased the speed of petal expansion from stage 2 to stage 5. The expressions of 11 cell expansion-related genes involved in cell wall loosening, cell turgor modulation, and cytoskeleton remodeling were significantly down-regulated in RhNF-YC9-silenced petals. We also found that silencing RhNF-YC9 decreased the expression of gibberellin acid (GA) biosynthetic gene RhGA20ox while significantly increasing the transcripts of GA catabolic gene RhGA2ox, reducing the accumulation of GA4 and GA7. The influence of ethylene treatment on the expression of RhGA20ox and RhGA2ox showed the same trend. These results together suggested that RhNF-YC9 positively regulated the speed of petal expansion and mediated the crosstalk between ethylene and GA. Our findings revealed a new insight into the function of NF-YC transcription factors involved in ethylene-regulated petal expansion. |
| first_indexed | 2024-04-24T08:17:37Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2468-0141 |
| language | English |
| last_indexed | 2024-04-24T08:17:37Z |
| publishDate | 2020-11-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj.art-db341fbc97b7417494fedf78b83c3da82024-04-17T03:11:04ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412020-11-0166419427An Ethylene-inhibited NF-YC Transcription Factor RhNF-YC9 Regulates Petal Expansion in RoseChangxi Chen0Nisar Hussain1Yaru Wang2Mingtong Li3Lin Liu4Meizhu Qin5Nan Ma6Junping Gao7Xiaoming Sun8Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, College of Horticulture, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, College of Horticulture, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, College of Horticulture, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, College of Horticulture, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, College of Horticulture, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, College of Horticulture, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, College of Horticulture, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, College of Horticulture, China Agricultural University, Beijing 100193, ChinaCorresponding author. Tel.: +86 10 62733603.; Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, College of Horticulture, China Agricultural University, Beijing 100193, ChinaThe speed of flower opening is closely related to their ornamental period. Ethylene functions as a negative regulator involved in the regulation of the petal expansion process. In this study, we isolated a NF-YC transcription factor gene, RhNF-YC9, from rose petals. RhNF-YC9 expression was induced at the early stages of flower opening but was inhibited by ethylene treatment. Silencing RhNF-YC9 decreased the speed of petal expansion from stage 2 to stage 5. The expressions of 11 cell expansion-related genes involved in cell wall loosening, cell turgor modulation, and cytoskeleton remodeling were significantly down-regulated in RhNF-YC9-silenced petals. We also found that silencing RhNF-YC9 decreased the expression of gibberellin acid (GA) biosynthetic gene RhGA20ox while significantly increasing the transcripts of GA catabolic gene RhGA2ox, reducing the accumulation of GA4 and GA7. The influence of ethylene treatment on the expression of RhGA20ox and RhGA2ox showed the same trend. These results together suggested that RhNF-YC9 positively regulated the speed of petal expansion and mediated the crosstalk between ethylene and GA. Our findings revealed a new insight into the function of NF-YC transcription factors involved in ethylene-regulated petal expansion.http://www.sciencedirect.com/science/article/pii/S2468014120301114Rosa hybridaPetal expansionEthyleneRhNF-YC9Transcriptional regulationGAs |
| spellingShingle | Changxi Chen Nisar Hussain Yaru Wang Mingtong Li Lin Liu Meizhu Qin Nan Ma Junping Gao Xiaoming Sun An Ethylene-inhibited NF-YC Transcription Factor RhNF-YC9 Regulates Petal Expansion in Rose Horticultural Plant Journal Rosa hybrida Petal expansion Ethylene RhNF-YC9 Transcriptional regulation GAs |
| title | An Ethylene-inhibited NF-YC Transcription Factor RhNF-YC9 Regulates Petal Expansion in Rose |
| title_full | An Ethylene-inhibited NF-YC Transcription Factor RhNF-YC9 Regulates Petal Expansion in Rose |
| title_fullStr | An Ethylene-inhibited NF-YC Transcription Factor RhNF-YC9 Regulates Petal Expansion in Rose |
| title_full_unstemmed | An Ethylene-inhibited NF-YC Transcription Factor RhNF-YC9 Regulates Petal Expansion in Rose |
| title_short | An Ethylene-inhibited NF-YC Transcription Factor RhNF-YC9 Regulates Petal Expansion in Rose |
| title_sort | ethylene inhibited nf yc transcription factor rhnf yc9 regulates petal expansion in rose |
| topic | Rosa hybrida Petal expansion Ethylene RhNF-YC9 Transcriptional regulation GAs |
| url | http://www.sciencedirect.com/science/article/pii/S2468014120301114 |
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