Improving Lipid Production of <i>Yarrowia lipolytica</i> by the Aldehyde Dehydrogenase-Mediated Furfural Detoxification
<i>Yarrowia lipolytica</i>, the non-conventional yeast capable of high lipogenesis, is a microbial chassis for producing lipid-based biofuels and chemicals from renewable resources such as lignocellulosic biomass. However, the low tolerance of <i>Y. lipolytica</i> against fur...
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MDPI AG
2022-04-01
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| Series: | International Journal of Molecular Sciences |
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| author | Jiwon Kim Hyeoncheol Francis Son Sungmin Hwang Gyeongtaek Gong Ja Kyong Ko Youngsoon Um Sung Ok Han Sun-Mi Lee |
| author_facet | Jiwon Kim Hyeoncheol Francis Son Sungmin Hwang Gyeongtaek Gong Ja Kyong Ko Youngsoon Um Sung Ok Han Sun-Mi Lee |
| author_sort | Jiwon Kim |
| collection | DOAJ |
| description | <i>Yarrowia lipolytica</i>, the non-conventional yeast capable of high lipogenesis, is a microbial chassis for producing lipid-based biofuels and chemicals from renewable resources such as lignocellulosic biomass. However, the low tolerance of <i>Y. lipolytica</i> against furfural, a major inhibitory furan aldehyde derived from the pretreatment processes of lignocellulosic biomass, has restricted the efficient conversion of lignocellulosic hydrolysates. In this study, the furfural tolerance of <i>Y. lipolytica</i> has been improved by supporting its endogenous detoxification mechanism. Specifically, the endogenous genes encoding the aldehyde dehydrogenase family proteins were overexpressed in <i>Y. lipolytica</i> to support the conversion of furfural to furoic acid. Among them, YALI0E15400p (FALDH2) has shown the highest conversion rate of furfural to furoic acid and resulted in two-fold increased cell growth and lipid production in the presence of 0.4 g/L of furfural. To our knowledge, this is the first report to identify the native furfural detoxification mechanism and increase furfural resistance through rational engineering in <i>Y. lipolytica</i>. Overall, these results will improve the potential of <i>Y. lipolytica</i> to produce lipids and other value-added chemicals from a carbon-neutral feedstock of lignocellulosic biomass. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T04:06:42Z |
| publishDate | 2022-04-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-db349bbe19e04f9a950375efeebf4bfb2023-11-23T08:21:53ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-04-01239476110.3390/ijms23094761Improving Lipid Production of <i>Yarrowia lipolytica</i> by the Aldehyde Dehydrogenase-Mediated Furfural DetoxificationJiwon Kim0Hyeoncheol Francis Son1Sungmin Hwang2Gyeongtaek Gong3Ja Kyong Ko4Youngsoon Um5Sung Ok Han6Sun-Mi Lee7Clean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, KoreaClean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, KoreaClean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, KoreaClean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, KoreaClean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, KoreaClean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, KoreaDepartment of Biotechnology, Korea University, Seoul 02841, KoreaClean Energy Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, Korea<i>Yarrowia lipolytica</i>, the non-conventional yeast capable of high lipogenesis, is a microbial chassis for producing lipid-based biofuels and chemicals from renewable resources such as lignocellulosic biomass. However, the low tolerance of <i>Y. lipolytica</i> against furfural, a major inhibitory furan aldehyde derived from the pretreatment processes of lignocellulosic biomass, has restricted the efficient conversion of lignocellulosic hydrolysates. In this study, the furfural tolerance of <i>Y. lipolytica</i> has been improved by supporting its endogenous detoxification mechanism. Specifically, the endogenous genes encoding the aldehyde dehydrogenase family proteins were overexpressed in <i>Y. lipolytica</i> to support the conversion of furfural to furoic acid. Among them, YALI0E15400p (FALDH2) has shown the highest conversion rate of furfural to furoic acid and resulted in two-fold increased cell growth and lipid production in the presence of 0.4 g/L of furfural. To our knowledge, this is the first report to identify the native furfural detoxification mechanism and increase furfural resistance through rational engineering in <i>Y. lipolytica</i>. Overall, these results will improve the potential of <i>Y. lipolytica</i> to produce lipids and other value-added chemicals from a carbon-neutral feedstock of lignocellulosic biomass.https://www.mdpi.com/1422-0067/23/9/4761<i>Yarrowia lipolytica</i>lipidsfurfuraltolerancealdehyde dehydrogenaseYALI0E15400p |
| spellingShingle | Jiwon Kim Hyeoncheol Francis Son Sungmin Hwang Gyeongtaek Gong Ja Kyong Ko Youngsoon Um Sung Ok Han Sun-Mi Lee Improving Lipid Production of <i>Yarrowia lipolytica</i> by the Aldehyde Dehydrogenase-Mediated Furfural Detoxification International Journal of Molecular Sciences <i>Yarrowia lipolytica</i> lipids furfural tolerance aldehyde dehydrogenase YALI0E15400p |
| title | Improving Lipid Production of <i>Yarrowia lipolytica</i> by the Aldehyde Dehydrogenase-Mediated Furfural Detoxification |
| title_full | Improving Lipid Production of <i>Yarrowia lipolytica</i> by the Aldehyde Dehydrogenase-Mediated Furfural Detoxification |
| title_fullStr | Improving Lipid Production of <i>Yarrowia lipolytica</i> by the Aldehyde Dehydrogenase-Mediated Furfural Detoxification |
| title_full_unstemmed | Improving Lipid Production of <i>Yarrowia lipolytica</i> by the Aldehyde Dehydrogenase-Mediated Furfural Detoxification |
| title_short | Improving Lipid Production of <i>Yarrowia lipolytica</i> by the Aldehyde Dehydrogenase-Mediated Furfural Detoxification |
| title_sort | improving lipid production of i yarrowia lipolytica i by the aldehyde dehydrogenase mediated furfural detoxification |
| topic | <i>Yarrowia lipolytica</i> lipids furfural tolerance aldehyde dehydrogenase YALI0E15400p |
| url | https://www.mdpi.com/1422-0067/23/9/4761 |
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