Population structure, genetic diversity and pathotypes of Streptococcus suis isolated during the last 13 years from diseased pigs in Switzerland
Abstract Streptococcus (S.) suis is a globally important swine pathogen, which comprises certain zoonotic serotypes. In this study, a detailed characterization of 88 porcine S. suis isolates was performed by analyzing capsular (cps) types, multilocus sequence typing (MLST) and investigation of the m...
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| Format: | Article |
| Language: | English |
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BMC
2020-07-01
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| Series: | Veterinary Research |
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| Online Access: | http://link.springer.com/article/10.1186/s13567-020-00813-w |
| _version_ | 1818317035035164672 |
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| author | Simone Scherrer Giuliana Rosato Nathalie Spoerry Serrano Marc J. A. Stevens Fenja Rademacher Jacques Schrenzel Marcelo Gottschalk Roger Stephan Sophie Peterhans |
| author_facet | Simone Scherrer Giuliana Rosato Nathalie Spoerry Serrano Marc J. A. Stevens Fenja Rademacher Jacques Schrenzel Marcelo Gottschalk Roger Stephan Sophie Peterhans |
| author_sort | Simone Scherrer |
| collection | DOAJ |
| description | Abstract Streptococcus (S.) suis is a globally important swine pathogen, which comprises certain zoonotic serotypes. In this study, a detailed characterization of 88 porcine S. suis isolates was performed by analyzing capsular (cps) types, multilocus sequence typing (MLST) and investigation of the minimum core genome (MCG). In order to focus on the virulence potential of presumable invasive disease-associated S. suis isolates, virulence-associated gene profiles were assessed followed by screening a chosen subset of S. suis strains with a molecular pathotyping tool. Results showed a high genetic variability within this strain collection. In total, seventeen cps types were identified with a predominance of cps type 9 (15.9%) and 6 (14.8%). MLST revealed 48 sequence types (STs) including 41 novel ones. The population structure of S. suis was heterogenous and isolates belonged to eight different clonal complexes (CCs) including CC28 (9.1%), CC1109 (8%), CC13/149 (6.8%), CC1237 (5.7%), CC1 (3.4%), CC17 (3.4%), CC87 (2.3%), and CC1112 (1.1%), whereas a significant portion of isolates (60.2%) could not be assigned to any described CCs. Virulence-associated markers, namely extracellular protein factor (epf), muramidase-released protein (mrp), and suilysin (sly), showed a link with STs rather than with cps types. With this study an expanded knowledge about the population structure and the genetic diversity of S. suis could be achieved, which helps to contribute to an optimal public health surveillance system by promoting a focus on strains with an increased virulence and zoonotic potential. |
| first_indexed | 2024-12-13T09:30:55Z |
| format | Article |
| id | doaj.art-db358acb1c414fe2af1ee51bdd467df5 |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-12-13T09:30:55Z |
| publishDate | 2020-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-db358acb1c414fe2af1ee51bdd467df52022-12-21T23:52:30ZengBMCVeterinary Research1297-97162020-07-0151111610.1186/s13567-020-00813-wPopulation structure, genetic diversity and pathotypes of Streptococcus suis isolated during the last 13 years from diseased pigs in SwitzerlandSimone Scherrer0Giuliana Rosato1Nathalie Spoerry Serrano2Marc J. A. Stevens3Fenja Rademacher4Jacques Schrenzel5Marcelo Gottschalk6Roger Stephan7Sophie Peterhans8Department of Veterinary Bacteriology, Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of ZurichInstitute for Veterinary Pathology, Vetsuisse Faculty, University of ZurichDepartment of Veterinary Bacteriology, Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of ZurichDepartment of Veterinary Bacteriology, Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of ZurichDepartment of Veterinary Bacteriology, Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of ZurichBacteriology Laboratory, Geneva University Hospitals and University of GenevaSwine and Poultry Infectious Diseases Research Center (CRIPA), Groupe de recherche sur les maladies infectieuses des animaux de production (GREMIP), Faculty of Veterinary Medicine, University of MontrealDepartment of Veterinary Bacteriology, Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of ZurichDepartment of Veterinary Bacteriology, Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of ZurichAbstract Streptococcus (S.) suis is a globally important swine pathogen, which comprises certain zoonotic serotypes. In this study, a detailed characterization of 88 porcine S. suis isolates was performed by analyzing capsular (cps) types, multilocus sequence typing (MLST) and investigation of the minimum core genome (MCG). In order to focus on the virulence potential of presumable invasive disease-associated S. suis isolates, virulence-associated gene profiles were assessed followed by screening a chosen subset of S. suis strains with a molecular pathotyping tool. Results showed a high genetic variability within this strain collection. In total, seventeen cps types were identified with a predominance of cps type 9 (15.9%) and 6 (14.8%). MLST revealed 48 sequence types (STs) including 41 novel ones. The population structure of S. suis was heterogenous and isolates belonged to eight different clonal complexes (CCs) including CC28 (9.1%), CC1109 (8%), CC13/149 (6.8%), CC1237 (5.7%), CC1 (3.4%), CC17 (3.4%), CC87 (2.3%), and CC1112 (1.1%), whereas a significant portion of isolates (60.2%) could not be assigned to any described CCs. Virulence-associated markers, namely extracellular protein factor (epf), muramidase-released protein (mrp), and suilysin (sly), showed a link with STs rather than with cps types. With this study an expanded knowledge about the population structure and the genetic diversity of S. suis could be achieved, which helps to contribute to an optimal public health surveillance system by promoting a focus on strains with an increased virulence and zoonotic potential.http://link.springer.com/article/10.1186/s13567-020-00813-wStreptococcus suisMLSTCapsular typeClonal complexVirulence potentialInvasive disease-associated |
| spellingShingle | Simone Scherrer Giuliana Rosato Nathalie Spoerry Serrano Marc J. A. Stevens Fenja Rademacher Jacques Schrenzel Marcelo Gottschalk Roger Stephan Sophie Peterhans Population structure, genetic diversity and pathotypes of Streptococcus suis isolated during the last 13 years from diseased pigs in Switzerland Veterinary Research Streptococcus suis MLST Capsular type Clonal complex Virulence potential Invasive disease-associated |
| title | Population structure, genetic diversity and pathotypes of Streptococcus suis isolated during the last 13 years from diseased pigs in Switzerland |
| title_full | Population structure, genetic diversity and pathotypes of Streptococcus suis isolated during the last 13 years from diseased pigs in Switzerland |
| title_fullStr | Population structure, genetic diversity and pathotypes of Streptococcus suis isolated during the last 13 years from diseased pigs in Switzerland |
| title_full_unstemmed | Population structure, genetic diversity and pathotypes of Streptococcus suis isolated during the last 13 years from diseased pigs in Switzerland |
| title_short | Population structure, genetic diversity and pathotypes of Streptococcus suis isolated during the last 13 years from diseased pigs in Switzerland |
| title_sort | population structure genetic diversity and pathotypes of streptococcus suis isolated during the last 13 years from diseased pigs in switzerland |
| topic | Streptococcus suis MLST Capsular type Clonal complex Virulence potential Invasive disease-associated |
| url | http://link.springer.com/article/10.1186/s13567-020-00813-w |
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