Detection of high PD-L1 expression in oral cancers by a novel monoclonal antibody L1Mab-4
Programmed cell death-ligand 1 (PD-L1), which is a ligand of programmed cell death-1 (PD-1), is a type I transmembrane glycoprotein that is expressed on antigen-presenting cells and several tumor cells, including melanoma and lung cancer cells. There is a strong correlation between human PD-L1 (hPD-...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-03-01
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| Series: | Biochemistry and Biophysics Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580818300049 |
| _version_ | 1819289445219696640 |
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| author | Shinji Yamada Shunsuke Itai Mika K. Kaneko Yukinari Kato |
| author_facet | Shinji Yamada Shunsuke Itai Mika K. Kaneko Yukinari Kato |
| author_sort | Shinji Yamada |
| collection | DOAJ |
| description | Programmed cell death-ligand 1 (PD-L1), which is a ligand of programmed cell death-1 (PD-1), is a type I transmembrane glycoprotein that is expressed on antigen-presenting cells and several tumor cells, including melanoma and lung cancer cells. There is a strong correlation between human PD-L1 (hPD-L1) expression on tumor cells and negative prognosis in cancer patients. In this study, we produced a novel anti-hPD-L1 monoclonal antibody (mAb), L1Mab-4 (IgG2b, kappa), using cell-based immunization and screening (CBIS) method and investigated hPD-L1 expression in oral cancers. L1Mab-4 reacted with oral cancer cell lines (Ca9-22, HO-1-u-1, SAS, HSC-2, HSC-3, and HSC-4) in flow cytometry and stained oral cancers in a membrane-staining pattern. L1Mab-4 stained 106/150 (70.7%) of oral squamous cell carcinomas, indicating the very high sensitivity of L1Mab-4. These results indicate that L1Mab-4 could be useful for investigating the function of hPD-L1 in oral cancers. Keywords: Programmed cell death-ligand 1, Monoclonal antibody, Oral cancer |
| first_indexed | 2024-12-24T03:06:57Z |
| format | Article |
| id | doaj.art-db35c3c29188487c977e954fbac36f52 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-24T03:06:57Z |
| publishDate | 2018-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-db35c3c29188487c977e954fbac36f522022-12-21T17:18:00ZengElsevierBiochemistry and Biophysics Reports2405-58082018-03-0113123128Detection of high PD-L1 expression in oral cancers by a novel monoclonal antibody L1Mab-4Shinji Yamada0Shunsuke Itai1Mika K. Kaneko2Yukinari Kato3Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, JapanDepartment of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, JapanDepartment of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, JapanCorrespondence to: New Industry Creation Hatchery Center, Tohoku University, 2-1, Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, JapanProgrammed cell death-ligand 1 (PD-L1), which is a ligand of programmed cell death-1 (PD-1), is a type I transmembrane glycoprotein that is expressed on antigen-presenting cells and several tumor cells, including melanoma and lung cancer cells. There is a strong correlation between human PD-L1 (hPD-L1) expression on tumor cells and negative prognosis in cancer patients. In this study, we produced a novel anti-hPD-L1 monoclonal antibody (mAb), L1Mab-4 (IgG2b, kappa), using cell-based immunization and screening (CBIS) method and investigated hPD-L1 expression in oral cancers. L1Mab-4 reacted with oral cancer cell lines (Ca9-22, HO-1-u-1, SAS, HSC-2, HSC-3, and HSC-4) in flow cytometry and stained oral cancers in a membrane-staining pattern. L1Mab-4 stained 106/150 (70.7%) of oral squamous cell carcinomas, indicating the very high sensitivity of L1Mab-4. These results indicate that L1Mab-4 could be useful for investigating the function of hPD-L1 in oral cancers. Keywords: Programmed cell death-ligand 1, Monoclonal antibody, Oral cancerhttp://www.sciencedirect.com/science/article/pii/S2405580818300049 |
| spellingShingle | Shinji Yamada Shunsuke Itai Mika K. Kaneko Yukinari Kato Detection of high PD-L1 expression in oral cancers by a novel monoclonal antibody L1Mab-4 Biochemistry and Biophysics Reports |
| title | Detection of high PD-L1 expression in oral cancers by a novel monoclonal antibody L1Mab-4 |
| title_full | Detection of high PD-L1 expression in oral cancers by a novel monoclonal antibody L1Mab-4 |
| title_fullStr | Detection of high PD-L1 expression in oral cancers by a novel monoclonal antibody L1Mab-4 |
| title_full_unstemmed | Detection of high PD-L1 expression in oral cancers by a novel monoclonal antibody L1Mab-4 |
| title_short | Detection of high PD-L1 expression in oral cancers by a novel monoclonal antibody L1Mab-4 |
| title_sort | detection of high pd l1 expression in oral cancers by a novel monoclonal antibody l1mab 4 |
| url | http://www.sciencedirect.com/science/article/pii/S2405580818300049 |
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