Studies on Natural Products Using Monoclonal Antibodies: A Review
An immunoblotting system (“eastern blotting”) was developed for small-molecule herbal medicines like glycosides, with no conjugation function to the membrane. Briefly, the crude extracts of herb medicines were developed by thin-layer chromatography (TLC). The small-molecule herbal medicines on TLC p...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Antibodies |
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| Online Access: | https://www.mdpi.com/2073-4468/10/4/43 |
| _version_ | 1797506899130712064 |
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| author | Yukihiro Shoyama |
| author_facet | Yukihiro Shoyama |
| author_sort | Yukihiro Shoyama |
| collection | DOAJ |
| description | An immunoblotting system (“eastern blotting”) was developed for small-molecule herbal medicines like glycosides, with no conjugation function to the membrane. Briefly, the crude extracts of herb medicines were developed by thin-layer chromatography (TLC). The small-molecule herbal medicines on TLC plates were transferred to polyvinylidene fluoride (PVDF) or polyethersulfone (PES) membranes by heating. Antigen components were divided into two categories based on their function, i.e., their membrane recognizing (aglycone part) and fixing (sugar moiety) abilities. This procedure allows for the staining of only target glycosides. Double eastern blotting was developed as a further staining system for two herb medicines using a set of MAbs and substrates. |
| first_indexed | 2024-03-10T04:39:00Z |
| format | Article |
| id | doaj.art-db36b5ab69094c31ad7c551749be28fc |
| institution | Directory Open Access Journal |
| issn | 2073-4468 |
| language | English |
| last_indexed | 2024-03-10T04:39:00Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibodies |
| spelling | doaj.art-db36b5ab69094c31ad7c551749be28fc2023-11-23T03:31:53ZengMDPI AGAntibodies2073-44682021-11-011044310.3390/antib10040043Studies on Natural Products Using Monoclonal Antibodies: A ReviewYukihiro Shoyama0Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298, JapanAn immunoblotting system (“eastern blotting”) was developed for small-molecule herbal medicines like glycosides, with no conjugation function to the membrane. Briefly, the crude extracts of herb medicines were developed by thin-layer chromatography (TLC). The small-molecule herbal medicines on TLC plates were transferred to polyvinylidene fluoride (PVDF) or polyethersulfone (PES) membranes by heating. Antigen components were divided into two categories based on their function, i.e., their membrane recognizing (aglycone part) and fixing (sugar moiety) abilities. This procedure allows for the staining of only target glycosides. Double eastern blotting was developed as a further staining system for two herb medicines using a set of MAbs and substrates.https://www.mdpi.com/2073-4468/10/4/43monoclonal antibodyglycosideseastern blottingimmunoaffinity separationknockout extract |
| spellingShingle | Yukihiro Shoyama Studies on Natural Products Using Monoclonal Antibodies: A Review Antibodies monoclonal antibody glycosides eastern blotting immunoaffinity separation knockout extract |
| title | Studies on Natural Products Using Monoclonal Antibodies: A Review |
| title_full | Studies on Natural Products Using Monoclonal Antibodies: A Review |
| title_fullStr | Studies on Natural Products Using Monoclonal Antibodies: A Review |
| title_full_unstemmed | Studies on Natural Products Using Monoclonal Antibodies: A Review |
| title_short | Studies on Natural Products Using Monoclonal Antibodies: A Review |
| title_sort | studies on natural products using monoclonal antibodies a review |
| topic | monoclonal antibody glycosides eastern blotting immunoaffinity separation knockout extract |
| url | https://www.mdpi.com/2073-4468/10/4/43 |
| work_keys_str_mv | AT yukihiroshoyama studiesonnaturalproductsusingmonoclonalantibodiesareview |