DIAGNOSTICATION OF MALIGNANT HYPERTHERMIA IN SWINE
Malignant hyperthermia (MH) causes neurological, liver, and kidney damage and death in humans and major economic losses in the swine industry. A single point mutation in the gene for the skeletal muscle ryanodine receptor (Ryrl) was found to be correlated with MH in major breeds of swine. This mutat...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Agroprint Timisoara
2023-11-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/1713 |
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| author | MARIA ADINA MANEA STELIANA KEVORKIAN S. E. GEORGESCU ANCA DINISCHIOTU MARIETA COSTACHE |
| author_facet | MARIA ADINA MANEA STELIANA KEVORKIAN S. E. GEORGESCU ANCA DINISCHIOTU MARIETA COSTACHE |
| author_sort | MARIA ADINA MANEA |
| collection | DOAJ |
| description | Malignant hyperthermia (MH) causes neurological, liver, and kidney damage and
death in humans and major economic losses in the swine industry. A single point
mutation in the gene for the skeletal muscle ryanodine receptor (Ryrl) was found to
be correlated with MH in major breeds of swine. This mutation generates the
production of an abnormal protein which alters the structure and function of the
Ca2+ release channel of skeletal muscle sarcoplasmic reticulum (ryanodine
receptor). Because the mutation destroys the recognition site for the restriction
endonuclease HhaI, it was possible to develop a PCR-RFLP method for MH
diagnostication in all swine breeds. Our objective was to identify the normal
homozygous, heterozygous carriers and affected homozygous swine for MH trait,
using this method. Results suggest that the genetic test will be useful in identifying
heterozygous pigs for the MH trait. |
| first_indexed | 2024-04-24T11:48:28Z |
| format | Article |
| id | doaj.art-db370d4ad78b4edfabebdc20f79ccd0c |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:48:28Z |
| publishDate | 2023-11-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-db370d4ad78b4edfabebdc20f79ccd0c2024-04-09T08:50:27ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-11-014111171171713DIAGNOSTICATION OF MALIGNANT HYPERTHERMIA IN SWINEMARIA ADINA MANEA0STELIANA KEVORKIAN1S. E. GEORGESCU2ANCA DINISCHIOTU3MARIETA COSTACHE4University of Bucharest, Molecular Biology Center University of Bucharest, Molecular Biology Center University of Bucharest, Molecular Biology Center University of Bucharest, Molecular Biology Center University of Bucharest, Molecular Biology Center Malignant hyperthermia (MH) causes neurological, liver, and kidney damage and death in humans and major economic losses in the swine industry. A single point mutation in the gene for the skeletal muscle ryanodine receptor (Ryrl) was found to be correlated with MH in major breeds of swine. This mutation generates the production of an abnormal protein which alters the structure and function of the Ca2+ release channel of skeletal muscle sarcoplasmic reticulum (ryanodine receptor). Because the mutation destroys the recognition site for the restriction endonuclease HhaI, it was possible to develop a PCR-RFLP method for MH diagnostication in all swine breeds. Our objective was to identify the normal homozygous, heterozygous carriers and affected homozygous swine for MH trait, using this method. Results suggest that the genetic test will be useful in identifying heterozygous pigs for the MH trait.https://spasb.ro/index.php/public_html/article/view/1713swinemalignant hyperthermiapcr-rflpdiagnostication |
| spellingShingle | MARIA ADINA MANEA STELIANA KEVORKIAN S. E. GEORGESCU ANCA DINISCHIOTU MARIETA COSTACHE DIAGNOSTICATION OF MALIGNANT HYPERTHERMIA IN SWINE Scientific Papers Animal Science and Biotechnologies swine malignant hyperthermia pcr-rflp diagnostication |
| title | DIAGNOSTICATION OF MALIGNANT HYPERTHERMIA IN SWINE |
| title_full | DIAGNOSTICATION OF MALIGNANT HYPERTHERMIA IN SWINE |
| title_fullStr | DIAGNOSTICATION OF MALIGNANT HYPERTHERMIA IN SWINE |
| title_full_unstemmed | DIAGNOSTICATION OF MALIGNANT HYPERTHERMIA IN SWINE |
| title_short | DIAGNOSTICATION OF MALIGNANT HYPERTHERMIA IN SWINE |
| title_sort | diagnostication of malignant hyperthermia in swine |
| topic | swine malignant hyperthermia pcr-rflp diagnostication |
| url | https://spasb.ro/index.php/public_html/article/view/1713 |
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