Surface Acoustic Wave Immunosensor for Detection of Botulinum Neurotoxin
A Love-type acoustic wave sensor (AT-cut quartz substrate, SiO<sub>2</sub> guiding layer) with a center frequency of approximately 120 MHz was used to detect a simulant of pathogenic botulinum neurotoxin type A—recombinant of BoNT-A light chain—in liquid samples. The sensor was prepared...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/23/18/7688 |
| _version_ | 1797577137928011776 |
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| author | Michał Grabka Krzysztof Jasek Zygfryd Witkiewicz |
| author_facet | Michał Grabka Krzysztof Jasek Zygfryd Witkiewicz |
| author_sort | Michał Grabka |
| collection | DOAJ |
| description | A Love-type acoustic wave sensor (AT-cut quartz substrate, SiO<sub>2</sub> guiding layer) with a center frequency of approximately 120 MHz was used to detect a simulant of pathogenic botulinum neurotoxin type A—recombinant of BoNT-A light chain—in liquid samples. The sensor was prepared by immobilizing monoclonal antibodies specific for botulinum neurotoxin via a thiol monolayer deposited on a gold substrate. Studies have shown that the sensor enables selective analyte detection within a few minutes. In addition, the sensor can be used several times (regeneration of the sensor is possible using a low pH buffer). Nevertheless, the detectability of the analyte is relatively low compared to other analytical techniques that can be used for rapid detection of botulinum neurotoxin. The obtained results confirm the operation of the proposed sensor and give hope for further development of this label-free technique for detecting botulinum neurotoxin. |
| first_indexed | 2024-03-10T22:03:45Z |
| format | Article |
| id | doaj.art-db38137147604ea1bb7ee1d69042c13a |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T22:03:45Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-db38137147604ea1bb7ee1d69042c13a2023-11-19T12:52:47ZengMDPI AGSensors1424-82202023-09-012318768810.3390/s23187688Surface Acoustic Wave Immunosensor for Detection of Botulinum NeurotoxinMichał Grabka0Krzysztof Jasek1Zygfryd Witkiewicz2Institute of Chemistry, Faculty of Advanced Technologies and Chemistry, Military University of Technology, 00-908 Warsaw, PolandInstitute of Chemistry, Faculty of Advanced Technologies and Chemistry, Military University of Technology, 00-908 Warsaw, PolandInstitute of Chemistry, Faculty of Advanced Technologies and Chemistry, Military University of Technology, 00-908 Warsaw, PolandA Love-type acoustic wave sensor (AT-cut quartz substrate, SiO<sub>2</sub> guiding layer) with a center frequency of approximately 120 MHz was used to detect a simulant of pathogenic botulinum neurotoxin type A—recombinant of BoNT-A light chain—in liquid samples. The sensor was prepared by immobilizing monoclonal antibodies specific for botulinum neurotoxin via a thiol monolayer deposited on a gold substrate. Studies have shown that the sensor enables selective analyte detection within a few minutes. In addition, the sensor can be used several times (regeneration of the sensor is possible using a low pH buffer). Nevertheless, the detectability of the analyte is relatively low compared to other analytical techniques that can be used for rapid detection of botulinum neurotoxin. The obtained results confirm the operation of the proposed sensor and give hope for further development of this label-free technique for detecting botulinum neurotoxin.https://www.mdpi.com/1424-8220/23/18/7688SAWlabel-free detectionbotulinum neurotoxinbiological warfare agents (BWA)on-site detection of BWAbiological aerosol |
| spellingShingle | Michał Grabka Krzysztof Jasek Zygfryd Witkiewicz Surface Acoustic Wave Immunosensor for Detection of Botulinum Neurotoxin Sensors SAW label-free detection botulinum neurotoxin biological warfare agents (BWA) on-site detection of BWA biological aerosol |
| title | Surface Acoustic Wave Immunosensor for Detection of Botulinum Neurotoxin |
| title_full | Surface Acoustic Wave Immunosensor for Detection of Botulinum Neurotoxin |
| title_fullStr | Surface Acoustic Wave Immunosensor for Detection of Botulinum Neurotoxin |
| title_full_unstemmed | Surface Acoustic Wave Immunosensor for Detection of Botulinum Neurotoxin |
| title_short | Surface Acoustic Wave Immunosensor for Detection of Botulinum Neurotoxin |
| title_sort | surface acoustic wave immunosensor for detection of botulinum neurotoxin |
| topic | SAW label-free detection botulinum neurotoxin biological warfare agents (BWA) on-site detection of BWA biological aerosol |
| url | https://www.mdpi.com/1424-8220/23/18/7688 |
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