Identification and Characterization of Triple Action Bioagents (TAB) and Their Potency against Fusarium Wilt of Lentil
<i>Fusarium</i> wilt is a severe disease that plays a significant role in reducing the yield of lentil. Under favorable conditions for disease growth, the disease can cause complete crop failure and can be a crucial limiting issue for lentil cultivation in specific geographical zones. Th...
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| Format: | Article |
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MDPI AG
2021-12-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/7/12/587 |
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| author | Raheeba Tun Nisa Khurshid Ahmad Bhat Umer Basu Rameesa Rashid Xiukang Wang Shaheen Kauser Jan H. S. Viswanath Walid Soufan Muntazir Mushtaq Muhammad Habib ur Rahman Farooq Ahmad bhat Ayman EL Sabagh |
| author_facet | Raheeba Tun Nisa Khurshid Ahmad Bhat Umer Basu Rameesa Rashid Xiukang Wang Shaheen Kauser Jan H. S. Viswanath Walid Soufan Muntazir Mushtaq Muhammad Habib ur Rahman Farooq Ahmad bhat Ayman EL Sabagh |
| author_sort | Raheeba Tun Nisa |
| collection | DOAJ |
| description | <i>Fusarium</i> wilt is a severe disease that plays a significant role in reducing the yield of lentil. Under favorable conditions for disease growth, the disease can cause complete crop failure and can be a crucial limiting issue for lentil cultivation in specific geographical zones. The current work focused on isolating potentialbio-agents exhibiting copper oxychloride resistance and evaluating their efficacy in seed treatment for ecologically sustainable management of <i>Fusarium</i> wilt of lentil. Seventy biocontrol agent isolates were isolated and tested for resistance by growing them on Potato Dextrose Agar medium (PDA) amended with copper oxychloride at the rate of 2500 ppm. Isolate-H10 and isolate-C9 showed more excellent compatibility with copper oxychloride fungicide with 69 mm and 65 mm radial growths, respectively. The isolates H10 and C9 had the highest inhibitory percentages of 84.30% and 83.94% against <i>Fusarium oxysporum</i> f. sp. <i>lentis</i>, respectively, and the highest phosphorus solubilization index (PSI). Primers (ITS 1 and ITS 4) identified these putative bioagents as <i>Trichoderma harzianum</i> isolate skua-tab-1 and <i>Penicillium crysogenum</i> strain Tab2. Sequences were submitted to the NCBI and assigned the accession numbers MK414603 and MK418066. In pot culture, these isolates also demonstrated their superiority in reducing the disease incidence and severity if seeds were treated with H10 and C9 alone or in combination with copper oxychloride fungicide. The two isolated bioagents exhibit three fundamental properties: compatibility with copper oxychloride, antagonistic activity toward the pathogen fall armyworm, and the ability to dissolve phosphorus minerals. |
| first_indexed | 2024-03-10T03:58:56Z |
| format | Article |
| id | doaj.art-db381d948a8f40ca8d11e72dd66cc416 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T03:58:56Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-db381d948a8f40ca8d11e72dd66cc4162023-11-23T08:38:40ZengMDPI AGHorticulturae2311-75242021-12-0171258710.3390/horticulturae7120587Identification and Characterization of Triple Action Bioagents (TAB) and Their Potency against Fusarium Wilt of LentilRaheeba Tun Nisa0Khurshid Ahmad Bhat1Umer Basu2Rameesa Rashid3Xiukang Wang4Shaheen Kauser Jan5H. S. Viswanath6Walid Soufan7Muntazir Mushtaq8Muhammad Habib ur Rahman9Farooq Ahmad bhat10Ayman EL Sabagh11Division of Plant Pathology, FoA, Sher-e-Kashmir University of Agricultural Sciences and Technology-Kashmir, Wadura Sopore, Srinagar 190025, IndiaDivision of Plant Pathology, FoA, Sher-e-Kashmir University of Agricultural Sciences and Technology-Kashmir, Wadura Sopore, Srinagar 190025, IndiaDivision of Plant Pathology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu 18009, IndiaRegional Research Institute of Unani Medicine Srinagar, Srinagar 190003, IndiaCollege of Life Sciences, Yan’an University, Yan’an 716000, ChinaDivision of Plant Pathology, FoA, Sher-e-Kashmir University of Agricultural Sciences and Technology-Kashmir, Wadura Sopore, Srinagar 190025, IndiaDivision of Plant Pathology, FoA, Sher-e-Kashmir University of Agricultural Sciences and Technology-Kashmir, Wadura Sopore, Srinagar 190025, IndiaPlant Production Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaDivision of Germplasm Evaluation, ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110012, IndiaInstitute of Crop Science and Resource Conservation (INRES), University of Bonn, 53113 Bonn, GermanyDivision of Plant Pathology, FoA, Sher-e-Kashmir University of Agricultural Sciences and Technology-Kashmir, Wadura Sopore, Srinagar 190025, IndiaDepartment of Agronomy, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh 33516, Egypt<i>Fusarium</i> wilt is a severe disease that plays a significant role in reducing the yield of lentil. Under favorable conditions for disease growth, the disease can cause complete crop failure and can be a crucial limiting issue for lentil cultivation in specific geographical zones. The current work focused on isolating potentialbio-agents exhibiting copper oxychloride resistance and evaluating their efficacy in seed treatment for ecologically sustainable management of <i>Fusarium</i> wilt of lentil. Seventy biocontrol agent isolates were isolated and tested for resistance by growing them on Potato Dextrose Agar medium (PDA) amended with copper oxychloride at the rate of 2500 ppm. Isolate-H10 and isolate-C9 showed more excellent compatibility with copper oxychloride fungicide with 69 mm and 65 mm radial growths, respectively. The isolates H10 and C9 had the highest inhibitory percentages of 84.30% and 83.94% against <i>Fusarium oxysporum</i> f. sp. <i>lentis</i>, respectively, and the highest phosphorus solubilization index (PSI). Primers (ITS 1 and ITS 4) identified these putative bioagents as <i>Trichoderma harzianum</i> isolate skua-tab-1 and <i>Penicillium crysogenum</i> strain Tab2. Sequences were submitted to the NCBI and assigned the accession numbers MK414603 and MK418066. In pot culture, these isolates also demonstrated their superiority in reducing the disease incidence and severity if seeds were treated with H10 and C9 alone or in combination with copper oxychloride fungicide. The two isolated bioagents exhibit three fundamental properties: compatibility with copper oxychloride, antagonistic activity toward the pathogen fall armyworm, and the ability to dissolve phosphorus minerals.https://www.mdpi.com/2311-7524/7/12/587bio-agentscopper oxychloridephosphorus solubilization<i>Fusarium</i> wiltlentil |
| spellingShingle | Raheeba Tun Nisa Khurshid Ahmad Bhat Umer Basu Rameesa Rashid Xiukang Wang Shaheen Kauser Jan H. S. Viswanath Walid Soufan Muntazir Mushtaq Muhammad Habib ur Rahman Farooq Ahmad bhat Ayman EL Sabagh Identification and Characterization of Triple Action Bioagents (TAB) and Their Potency against Fusarium Wilt of Lentil Horticulturae bio-agents copper oxychloride phosphorus solubilization <i>Fusarium</i> wilt lentil |
| title | Identification and Characterization of Triple Action Bioagents (TAB) and Their Potency against Fusarium Wilt of Lentil |
| title_full | Identification and Characterization of Triple Action Bioagents (TAB) and Their Potency against Fusarium Wilt of Lentil |
| title_fullStr | Identification and Characterization of Triple Action Bioagents (TAB) and Their Potency against Fusarium Wilt of Lentil |
| title_full_unstemmed | Identification and Characterization of Triple Action Bioagents (TAB) and Their Potency against Fusarium Wilt of Lentil |
| title_short | Identification and Characterization of Triple Action Bioagents (TAB) and Their Potency against Fusarium Wilt of Lentil |
| title_sort | identification and characterization of triple action bioagents tab and their potency against fusarium wilt of lentil |
| topic | bio-agents copper oxychloride phosphorus solubilization <i>Fusarium</i> wilt lentil |
| url | https://www.mdpi.com/2311-7524/7/12/587 |
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