Effects of Low Intensity Pulsed Ultrasound Stimulation on the Temporal Dynamics of Irradiated Bone Tissue Healing: A Histomorphometric Study in Rabbits
The present study evaluated the influence of Low-Intensity Pulsed Ultrasound (LIPUS) on the regeneration processes of non-critical-size bone defects in irradiated and non-irradiated rabbit tibias. Bone defects were surgically created on both tibiae of six rabbits. The control group had no additional...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/20/12426 |
| _version_ | 1797431588727816192 |
|---|---|
| author | Bob Biewer Felix Kleine-Borgmann Gaël P. Hammer Eric H. Rompen Michel Mittelbronn Pascale Quatresooz |
| author_facet | Bob Biewer Felix Kleine-Borgmann Gaël P. Hammer Eric H. Rompen Michel Mittelbronn Pascale Quatresooz |
| author_sort | Bob Biewer |
| collection | DOAJ |
| description | The present study evaluated the influence of Low-Intensity Pulsed Ultrasound (LIPUS) on the regeneration processes of non-critical-size bone defects in irradiated and non-irradiated rabbit tibias. Bone defects were surgically created on both tibiae of six rabbits. The control group had no additional treatment. In one intervention group, one tibia was irradiated with 15 Gy in a single dose. A second group was treated with LIPUS, and a third with a combination of both treatments. The control samples showed 83.10% ± 17.79% of bone repair after 9 weeks, while the irradiated bone had regenerated significantly less during the same period (66.42% ± 29.36%). The LIPUS treatment on irradiated bones performed a 79.21% ± 21.07% bone fill and could not significantly improve the response compared to the non-treated irradiated specimens. However, LIPUS treatment on non-irradiated bone showed bone formations beyond the size defect (115.91% ± 33.69%), which was a highly significant increase when compared to the control group or any irradiated group. The application of ultrasound to healthy bone produced highly significant and enhanced bone formations with 36.70% more regenerated bone when compared to the same application on irradiated bone. LIPUS vibration stimuli may be considered as a promising complementary treatment approach in non-irradiated bone regeneration procedures to shorten the treatment and enhance bone healing. In irradiated bones, the effect of ultrasound application is less clear, and further studies are needed to refine the dynamics of the present results. |
| first_indexed | 2024-03-09T09:46:13Z |
| format | Article |
| id | doaj.art-db39de04cdb7489fb0fad8a4122a46bd |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T09:46:13Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-db39de04cdb7489fb0fad8a4122a46bd2023-12-02T00:32:46ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123201242610.3390/ijms232012426Effects of Low Intensity Pulsed Ultrasound Stimulation on the Temporal Dynamics of Irradiated Bone Tissue Healing: A Histomorphometric Study in RabbitsBob Biewer0Felix Kleine-Borgmann1Gaël P. Hammer2Eric H. Rompen3Michel Mittelbronn4Pascale Quatresooz5Department of Periodontology and Oral Surgery, Faculty of Medicine, 4000 Liège, BelgiumLuxembourg Center of Neuropathology (LCNP), 3555 Dudelange, LuxembourgNational Center of Pathology (NCP), Laboratoire National de Santé (LNS), 3555 Dudelange, LuxembourgDepartment of Periodontology and Oral Surgery, Faculty of Medicine, 4000 Liège, BelgiumDepartment of Life Science and Medicine (DLSM), University of Luxembourg, 4365 Esch/Alzette, LuxembourgDepartment of Human Histology and Dermatopathology, University Hospital of Liège University (CHU), 4000 Liège, BelgiumThe present study evaluated the influence of Low-Intensity Pulsed Ultrasound (LIPUS) on the regeneration processes of non-critical-size bone defects in irradiated and non-irradiated rabbit tibias. Bone defects were surgically created on both tibiae of six rabbits. The control group had no additional treatment. In one intervention group, one tibia was irradiated with 15 Gy in a single dose. A second group was treated with LIPUS, and a third with a combination of both treatments. The control samples showed 83.10% ± 17.79% of bone repair after 9 weeks, while the irradiated bone had regenerated significantly less during the same period (66.42% ± 29.36%). The LIPUS treatment on irradiated bones performed a 79.21% ± 21.07% bone fill and could not significantly improve the response compared to the non-treated irradiated specimens. However, LIPUS treatment on non-irradiated bone showed bone formations beyond the size defect (115.91% ± 33.69%), which was a highly significant increase when compared to the control group or any irradiated group. The application of ultrasound to healthy bone produced highly significant and enhanced bone formations with 36.70% more regenerated bone when compared to the same application on irradiated bone. LIPUS vibration stimuli may be considered as a promising complementary treatment approach in non-irradiated bone regeneration procedures to shorten the treatment and enhance bone healing. In irradiated bones, the effect of ultrasound application is less clear, and further studies are needed to refine the dynamics of the present results.https://www.mdpi.com/1422-0067/23/20/12426low intensity pulsed ultrasoundosteogenesisosteoradionecrosisbone regenerationtibiarabbit |
| spellingShingle | Bob Biewer Felix Kleine-Borgmann Gaël P. Hammer Eric H. Rompen Michel Mittelbronn Pascale Quatresooz Effects of Low Intensity Pulsed Ultrasound Stimulation on the Temporal Dynamics of Irradiated Bone Tissue Healing: A Histomorphometric Study in Rabbits International Journal of Molecular Sciences low intensity pulsed ultrasound osteogenesis osteoradionecrosis bone regeneration tibia rabbit |
| title | Effects of Low Intensity Pulsed Ultrasound Stimulation on the Temporal Dynamics of Irradiated Bone Tissue Healing: A Histomorphometric Study in Rabbits |
| title_full | Effects of Low Intensity Pulsed Ultrasound Stimulation on the Temporal Dynamics of Irradiated Bone Tissue Healing: A Histomorphometric Study in Rabbits |
| title_fullStr | Effects of Low Intensity Pulsed Ultrasound Stimulation on the Temporal Dynamics of Irradiated Bone Tissue Healing: A Histomorphometric Study in Rabbits |
| title_full_unstemmed | Effects of Low Intensity Pulsed Ultrasound Stimulation on the Temporal Dynamics of Irradiated Bone Tissue Healing: A Histomorphometric Study in Rabbits |
| title_short | Effects of Low Intensity Pulsed Ultrasound Stimulation on the Temporal Dynamics of Irradiated Bone Tissue Healing: A Histomorphometric Study in Rabbits |
| title_sort | effects of low intensity pulsed ultrasound stimulation on the temporal dynamics of irradiated bone tissue healing a histomorphometric study in rabbits |
| topic | low intensity pulsed ultrasound osteogenesis osteoradionecrosis bone regeneration tibia rabbit |
| url | https://www.mdpi.com/1422-0067/23/20/12426 |
| work_keys_str_mv | AT bobbiewer effectsoflowintensitypulsedultrasoundstimulationonthetemporaldynamicsofirradiatedbonetissuehealingahistomorphometricstudyinrabbits AT felixkleineborgmann effectsoflowintensitypulsedultrasoundstimulationonthetemporaldynamicsofirradiatedbonetissuehealingahistomorphometricstudyinrabbits AT gaelphammer effectsoflowintensitypulsedultrasoundstimulationonthetemporaldynamicsofirradiatedbonetissuehealingahistomorphometricstudyinrabbits AT erichrompen effectsoflowintensitypulsedultrasoundstimulationonthetemporaldynamicsofirradiatedbonetissuehealingahistomorphometricstudyinrabbits AT michelmittelbronn effectsoflowintensitypulsedultrasoundstimulationonthetemporaldynamicsofirradiatedbonetissuehealingahistomorphometricstudyinrabbits AT pascalequatresooz effectsoflowintensitypulsedultrasoundstimulationonthetemporaldynamicsofirradiatedbonetissuehealingahistomorphometricstudyinrabbits |