Serogroup identification and Virulence gene characterization of Listeria monocytogenes isolated from chicken carcasses
Listeria monocytogenes is an important food borne pathogen with a high case fatality rate. Among 13 different serotypes of this bacterium, 4 serotypes (1/2a, 1/2b, 1/2c, and 4b) were considered as the main cause of listeriosis outbreaks. The aim of this study is to identify the major serotypes and v...
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| Format: | Article |
| Language: | English |
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Ferdowsi University of Mashhad
2015-10-01
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| Series: | The Iranian Journal of Veterinary Science and Technology |
| Subjects: | |
| Online Access: | https://ijvst.um.ac.ir/article_27823_26d4a1c7be9adf3bebc11046fd825e32.pdf |
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| author | Tayebeh Zeinali Abdolah Jamshidi Mohammadreza Bassami Mehrnaz Rad |
| author_facet | Tayebeh Zeinali Abdolah Jamshidi Mohammadreza Bassami Mehrnaz Rad |
| author_sort | Tayebeh Zeinali |
| collection | DOAJ |
| description | Listeria monocytogenes is an important food borne pathogen with a high case fatality rate. Among 13 different serotypes of this bacterium, 4 serotypes (1/2a, 1/2b, 1/2c, and 4b) were considered as the main cause of listeriosis outbreaks. The aim of this study is to identify the major serotypes and virulence genes in Listeria monocytegenes isolated from chicken carcasses which were collected from different supermarkets and butcheries in Mashhad. Among the 80 isolated Listeria spp., most of them were identified as L. monocytogenes (36 out of 80). Most of the L. monocytogenes isolates belonged to serogroup llb (52.77%) which contains 1/2b and 3b serotypes. The second and third major serogroups were IVa (27.77%) and IIa (16.66%). Serogroup IVb (2.77%) which contains 4b serotype was the fourth major isolate. In order to differentiate serotype 1/2a and 3a from 1/2c, amplification of flaA gene was used. L. monocytogenesis isolates were also examined for the presence of inlC, inlJ and hlyA virulence genes. 26 out of 36 isolates were positive for inlC, and inlJ, whereas hlyA gene was detected in 32 isolates. Chicken carcasses may act as a source of infectious listeriosis for humans living in this area. |
| first_indexed | 2024-12-10T11:10:03Z |
| format | Article |
| id | doaj.art-db3a503e20e9482fb3212579376e831b |
| institution | Directory Open Access Journal |
| issn | 2008-465X 2423-6306 |
| language | English |
| last_indexed | 2024-12-10T11:10:03Z |
| publishDate | 2015-10-01 |
| publisher | Ferdowsi University of Mashhad |
| record_format | Article |
| series | The Iranian Journal of Veterinary Science and Technology |
| spelling | doaj.art-db3a503e20e9482fb3212579376e831b2022-12-22T01:51:27ZengFerdowsi University of MashhadThe Iranian Journal of Veterinary Science and Technology2008-465X2423-63062015-10-017291910.22067/veterinary.v7i2.4365827823Serogroup identification and Virulence gene characterization of Listeria monocytogenes isolated from chicken carcassesTayebeh Zeinali0Abdolah Jamshidi1Mohammadreza Bassami2Mehrnaz Rad3Ferdowsi university of mashhadFerdowsi university of mashhadFerdowsi university of mashhadferdowsi university of mashhadListeria monocytogenes is an important food borne pathogen with a high case fatality rate. Among 13 different serotypes of this bacterium, 4 serotypes (1/2a, 1/2b, 1/2c, and 4b) were considered as the main cause of listeriosis outbreaks. The aim of this study is to identify the major serotypes and virulence genes in Listeria monocytegenes isolated from chicken carcasses which were collected from different supermarkets and butcheries in Mashhad. Among the 80 isolated Listeria spp., most of them were identified as L. monocytogenes (36 out of 80). Most of the L. monocytogenes isolates belonged to serogroup llb (52.77%) which contains 1/2b and 3b serotypes. The second and third major serogroups were IVa (27.77%) and IIa (16.66%). Serogroup IVb (2.77%) which contains 4b serotype was the fourth major isolate. In order to differentiate serotype 1/2a and 3a from 1/2c, amplification of flaA gene was used. L. monocytogenesis isolates were also examined for the presence of inlC, inlJ and hlyA virulence genes. 26 out of 36 isolates were positive for inlC, and inlJ, whereas hlyA gene was detected in 32 isolates. Chicken carcasses may act as a source of infectious listeriosis for humans living in this area.https://ijvst.um.ac.ir/article_27823_26d4a1c7be9adf3bebc11046fd825e32.pdfl. monocytogenesserotypeinlcinljhlya |
| spellingShingle | Tayebeh Zeinali Abdolah Jamshidi Mohammadreza Bassami Mehrnaz Rad Serogroup identification and Virulence gene characterization of Listeria monocytogenes isolated from chicken carcasses The Iranian Journal of Veterinary Science and Technology l. monocytogenes serotype inlc inlj hlya |
| title | Serogroup identification and Virulence gene characterization of Listeria monocytogenes isolated from chicken carcasses |
| title_full | Serogroup identification and Virulence gene characterization of Listeria monocytogenes isolated from chicken carcasses |
| title_fullStr | Serogroup identification and Virulence gene characterization of Listeria monocytogenes isolated from chicken carcasses |
| title_full_unstemmed | Serogroup identification and Virulence gene characterization of Listeria monocytogenes isolated from chicken carcasses |
| title_short | Serogroup identification and Virulence gene characterization of Listeria monocytogenes isolated from chicken carcasses |
| title_sort | serogroup identification and virulence gene characterization of listeria monocytogenes isolated from chicken carcasses |
| topic | l. monocytogenes serotype inlc inlj hlya |
| url | https://ijvst.um.ac.ir/article_27823_26d4a1c7be9adf3bebc11046fd825e32.pdf |
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