Efficient discrimination by MIRU-VNTRs of Mycobacterium tuberculosis clinical isolates belonging to the predominant SIT11/EAI3-IND ancestral genotypic lineage in Kerala, India
The present study evaluated the ability of MIRU–VNTRs to discriminate Mycobacterium tuberculosis (MTB) clinical isolates belonging to the SIT11/EAI3-IND ancestral genotypic lineage, which is highly prevalent in Kerala, India. Starting from 168 MTB clinical isolates, spoligotyping (discriminatory ind...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2013-01-01
|
| Series: | International Journal of Mycobacteriology |
| Subjects: | |
| Online Access: | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2013;volume=2;issue=4;spage=244;epage=247;aulast=Joseph |
| _version_ | 1828382532036460544 |
|---|---|
| author | Biljo V Joseph Smitha Soman Véronique Hill R Ajay Kumar Nalin Rastogi Sathish Mundayoor |
| author_facet | Biljo V Joseph Smitha Soman Véronique Hill R Ajay Kumar Nalin Rastogi Sathish Mundayoor |
| author_sort | Biljo V Joseph |
| collection | DOAJ |
| description | The present study evaluated the ability of MIRU–VNTRs to discriminate Mycobacterium tuberculosis (MTB) clinical isolates belonging to the SIT11/EAI3-IND ancestral genotypic lineage, which is highly prevalent in Kerala, India. Starting from 168 MTB clinical isolates, spoligotyping (discriminatory index of 0.9113) differentiated the strains into 68 distinct patterns, the biggest cluster being SIT11/48 SIT11 (n = 48). The present study shows that 12-loci MIRUs and 3 ETRs allowed an efficient discrimination of these isolates (discriminatory indexes of 0.7819 and 0.5523, respectively). |
| first_indexed | 2024-12-10T04:33:27Z |
| format | Article |
| id | doaj.art-db3b7b55258c471fae9db1cf0fcc0de7 |
| institution | Directory Open Access Journal |
| issn | 2212-5531 2212-554X |
| language | English |
| last_indexed | 2024-12-10T04:33:27Z |
| publishDate | 2013-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | International Journal of Mycobacteriology |
| spelling | doaj.art-db3b7b55258c471fae9db1cf0fcc0de72022-12-22T02:02:04ZengWolters Kluwer Medknow PublicationsInternational Journal of Mycobacteriology2212-55312212-554X2013-01-012424424710.1016/j.ijmyco.2013.10.005Efficient discrimination by MIRU-VNTRs of Mycobacterium tuberculosis clinical isolates belonging to the predominant SIT11/EAI3-IND ancestral genotypic lineage in Kerala, IndiaBiljo V JosephSmitha SomanVéronique HillR Ajay KumarNalin RastogiSathish MundayoorThe present study evaluated the ability of MIRU–VNTRs to discriminate Mycobacterium tuberculosis (MTB) clinical isolates belonging to the SIT11/EAI3-IND ancestral genotypic lineage, which is highly prevalent in Kerala, India. Starting from 168 MTB clinical isolates, spoligotyping (discriminatory index of 0.9113) differentiated the strains into 68 distinct patterns, the biggest cluster being SIT11/48 SIT11 (n = 48). The present study shows that 12-loci MIRUs and 3 ETRs allowed an efficient discrimination of these isolates (discriminatory indexes of 0.7819 and 0.5523, respectively).http://www.ijmyco.org/article.asp?issn=2212-5531;year=2013;volume=2;issue=4;spage=244;epage=247;aulast=JosephMycobacterium tuberculosisKeralaIndiaIS6110-RFLPMIRU-VNTRSpoligotyping |
| spellingShingle | Biljo V Joseph Smitha Soman Véronique Hill R Ajay Kumar Nalin Rastogi Sathish Mundayoor Efficient discrimination by MIRU-VNTRs of Mycobacterium tuberculosis clinical isolates belonging to the predominant SIT11/EAI3-IND ancestral genotypic lineage in Kerala, India International Journal of Mycobacteriology Mycobacterium tuberculosis Kerala India IS6110-RFLP MIRU-VNTR Spoligotyping |
| title | Efficient discrimination by MIRU-VNTRs of Mycobacterium tuberculosis clinical isolates belonging to the predominant SIT11/EAI3-IND ancestral genotypic lineage in Kerala, India |
| title_full | Efficient discrimination by MIRU-VNTRs of Mycobacterium tuberculosis clinical isolates belonging to the predominant SIT11/EAI3-IND ancestral genotypic lineage in Kerala, India |
| title_fullStr | Efficient discrimination by MIRU-VNTRs of Mycobacterium tuberculosis clinical isolates belonging to the predominant SIT11/EAI3-IND ancestral genotypic lineage in Kerala, India |
| title_full_unstemmed | Efficient discrimination by MIRU-VNTRs of Mycobacterium tuberculosis clinical isolates belonging to the predominant SIT11/EAI3-IND ancestral genotypic lineage in Kerala, India |
| title_short | Efficient discrimination by MIRU-VNTRs of Mycobacterium tuberculosis clinical isolates belonging to the predominant SIT11/EAI3-IND ancestral genotypic lineage in Kerala, India |
| title_sort | efficient discrimination by miru vntrs of mycobacterium tuberculosis clinical isolates belonging to the predominant sit11 eai3 ind ancestral genotypic lineage in kerala india |
| topic | Mycobacterium tuberculosis Kerala India IS6110-RFLP MIRU-VNTR Spoligotyping |
| url | http://www.ijmyco.org/article.asp?issn=2212-5531;year=2013;volume=2;issue=4;spage=244;epage=247;aulast=Joseph |
| work_keys_str_mv | AT biljovjoseph efficientdiscriminationbymiruvntrsofmycobacteriumtuberculosisclinicalisolatesbelongingtothepredominantsit11eai3indancestralgenotypiclineageinkeralaindia AT smithasoman efficientdiscriminationbymiruvntrsofmycobacteriumtuberculosisclinicalisolatesbelongingtothepredominantsit11eai3indancestralgenotypiclineageinkeralaindia AT veroniquehill efficientdiscriminationbymiruvntrsofmycobacteriumtuberculosisclinicalisolatesbelongingtothepredominantsit11eai3indancestralgenotypiclineageinkeralaindia AT rajaykumar efficientdiscriminationbymiruvntrsofmycobacteriumtuberculosisclinicalisolatesbelongingtothepredominantsit11eai3indancestralgenotypiclineageinkeralaindia AT nalinrastogi efficientdiscriminationbymiruvntrsofmycobacteriumtuberculosisclinicalisolatesbelongingtothepredominantsit11eai3indancestralgenotypiclineageinkeralaindia AT sathishmundayoor efficientdiscriminationbymiruvntrsofmycobacteriumtuberculosisclinicalisolatesbelongingtothepredominantsit11eai3indancestralgenotypiclineageinkeralaindia |