Intratumoral Monocyte Transfer to Examine Monocyte Differentiation in the Tumor Microenvironment
Summary: The regulation of monocyte differentiation in the tumor microenvironment is of significant interest to tumor immunologists. Monocytes injected into the circulation may not track into tumors in sufficient numbers, making intratumoral injections a preferred experimental approach. Monocyte enr...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720301751 |
| _version_ | 1818601541287804928 |
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| author | Samir Devalaraja Malay Haldar |
| author_facet | Samir Devalaraja Malay Haldar |
| author_sort | Samir Devalaraja |
| collection | DOAJ |
| description | Summary: The regulation of monocyte differentiation in the tumor microenvironment is of significant interest to tumor immunologists. Monocytes injected into the circulation may not track into tumors in sufficient numbers, making intratumoral injections a preferred experimental approach. Monocyte enrichment with antibody-based positive selection may activate downstream signaling, while cell sorters expose monocytes to mechanical stress. Here, we describe an approach of intratumoral monocyte transfer that circumvents these limitations by using negative selection and fluorescent reporter mice.For complete details on the use and execution of this protocol, please refer to Devalaraja et al. (2020). |
| first_indexed | 2024-12-16T12:53:01Z |
| format | Article |
| id | doaj.art-db40009b60074dd38239f69e3f801978 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-16T12:53:01Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-db40009b60074dd38239f69e3f8019782022-12-21T22:31:06ZengElsevierSTAR Protocols2666-16672020-12-0113100188Intratumoral Monocyte Transfer to Examine Monocyte Differentiation in the Tumor MicroenvironmentSamir Devalaraja0Malay Haldar1Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Abramson Family Cancer Research Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Institute for Immunology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USADepartment of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Abramson Family Cancer Research Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Institute for Immunology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Corresponding authorSummary: The regulation of monocyte differentiation in the tumor microenvironment is of significant interest to tumor immunologists. Monocytes injected into the circulation may not track into tumors in sufficient numbers, making intratumoral injections a preferred experimental approach. Monocyte enrichment with antibody-based positive selection may activate downstream signaling, while cell sorters expose monocytes to mechanical stress. Here, we describe an approach of intratumoral monocyte transfer that circumvents these limitations by using negative selection and fluorescent reporter mice.For complete details on the use and execution of this protocol, please refer to Devalaraja et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166720301751Cell isolationFlow Cytometry/Mass CytometryCancerImmunology |
| spellingShingle | Samir Devalaraja Malay Haldar Intratumoral Monocyte Transfer to Examine Monocyte Differentiation in the Tumor Microenvironment STAR Protocols Cell isolation Flow Cytometry/Mass Cytometry Cancer Immunology |
| title | Intratumoral Monocyte Transfer to Examine Monocyte Differentiation in the Tumor Microenvironment |
| title_full | Intratumoral Monocyte Transfer to Examine Monocyte Differentiation in the Tumor Microenvironment |
| title_fullStr | Intratumoral Monocyte Transfer to Examine Monocyte Differentiation in the Tumor Microenvironment |
| title_full_unstemmed | Intratumoral Monocyte Transfer to Examine Monocyte Differentiation in the Tumor Microenvironment |
| title_short | Intratumoral Monocyte Transfer to Examine Monocyte Differentiation in the Tumor Microenvironment |
| title_sort | intratumoral monocyte transfer to examine monocyte differentiation in the tumor microenvironment |
| topic | Cell isolation Flow Cytometry/Mass Cytometry Cancer Immunology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166720301751 |
| work_keys_str_mv | AT samirdevalaraja intratumoralmonocytetransfertoexaminemonocytedifferentiationinthetumormicroenvironment AT malayhaldar intratumoralmonocytetransfertoexaminemonocytedifferentiationinthetumormicroenvironment |