Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, <i>Cupriavidus necator</i> IBP/SFU-1, from Various Carbon Sources
The bacterial strain isolated from soil was identified as <i>Cupriavidus necator</i> IBP/SFU-1 and investigated as a PHA producer. The strain was found to be able to grow and synthesize PHAs under autotrophic conditions and showed a broad organotrophic potential towards different carbon...
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MDPI AG
2021-09-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/18/3142 |
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| author | Natalia O. Zhila Kristina Yu. Sapozhnikova Evgeniy G. Kiselev Alexander D. Vasiliev Ivan V. Nemtsev Ekaterina I. Shishatskaya Tatiana G. Volova |
| author_facet | Natalia O. Zhila Kristina Yu. Sapozhnikova Evgeniy G. Kiselev Alexander D. Vasiliev Ivan V. Nemtsev Ekaterina I. Shishatskaya Tatiana G. Volova |
| author_sort | Natalia O. Zhila |
| collection | DOAJ |
| description | The bacterial strain isolated from soil was identified as <i>Cupriavidus necator</i> IBP/SFU-1 and investigated as a PHA producer. The strain was found to be able to grow and synthesize PHAs under autotrophic conditions and showed a broad organotrophic potential towards different carbon sources: sugars, glycerol, fatty acids, and plant oils. The highest cell concentrations (7–8 g/L) and PHA contents were produced from oleic acid (78%), fructose, glucose, and palm oil (over 80%). The type of the carbon source influenced the PHA chemical composition and properties: when grown on oleic acid, the strain synthesized the P(3HB-co-3HV) copolymer; on plant oils, the P(3HB-co-3HV-co-3HHx) terpolymer, and on the other substrates, the P(3HB) homopolymer. The type of the carbon source influenced molecular-weight properties of PHAs: P(3HB) synthesized under autotrophic growth conditions, from CO<sub>2</sub>, had the highest number-average (290 ± 15 kDa) and weight-average (850 ± 25 kDa) molecular weights and the lowest polydispersity (2.9 ± 0.2); polymers synthesized from organic carbon sources showed increased polydispersity and reduced molecular weight. The carbon source was not found to affect the degree of crystallinity and thermal properties of the PHAs. The type of the carbon source determined not only PHA composition and molecular weight but also surface microstructure and porosity of the polymer films. The new strain can be recommended as a promising P(3HB) producer from palm oil, oleic acid, and sugars (fructose and glucose) and as a producer of P(3HB-co-3HV) from oleic acid and P(3HB-co-3HV-co-3HHx) from palm oil. |
| first_indexed | 2024-03-10T07:16:52Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T07:16:52Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-db4013996d3144db821e2adefcbf796d2023-11-22T14:56:46ZengMDPI AGPolymers2073-43602021-09-011318314210.3390/polym13183142Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, <i>Cupriavidus necator</i> IBP/SFU-1, from Various Carbon SourcesNatalia O. Zhila0Kristina Yu. Sapozhnikova1Evgeniy G. Kiselev2Alexander D. Vasiliev3Ivan V. Nemtsev4Ekaterina I. Shishatskaya5Tatiana G. Volova6Basic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., 660041 Krasnoyarsk, RussiaBasic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., 660041 Krasnoyarsk, RussiaBasic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., 660041 Krasnoyarsk, RussiaBasic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., 660041 Krasnoyarsk, RussiaBasic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., 660041 Krasnoyarsk, RussiaBasic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., 660041 Krasnoyarsk, RussiaBasic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., 660041 Krasnoyarsk, RussiaThe bacterial strain isolated from soil was identified as <i>Cupriavidus necator</i> IBP/SFU-1 and investigated as a PHA producer. The strain was found to be able to grow and synthesize PHAs under autotrophic conditions and showed a broad organotrophic potential towards different carbon sources: sugars, glycerol, fatty acids, and plant oils. The highest cell concentrations (7–8 g/L) and PHA contents were produced from oleic acid (78%), fructose, glucose, and palm oil (over 80%). The type of the carbon source influenced the PHA chemical composition and properties: when grown on oleic acid, the strain synthesized the P(3HB-co-3HV) copolymer; on plant oils, the P(3HB-co-3HV-co-3HHx) terpolymer, and on the other substrates, the P(3HB) homopolymer. The type of the carbon source influenced molecular-weight properties of PHAs: P(3HB) synthesized under autotrophic growth conditions, from CO<sub>2</sub>, had the highest number-average (290 ± 15 kDa) and weight-average (850 ± 25 kDa) molecular weights and the lowest polydispersity (2.9 ± 0.2); polymers synthesized from organic carbon sources showed increased polydispersity and reduced molecular weight. The carbon source was not found to affect the degree of crystallinity and thermal properties of the PHAs. The type of the carbon source determined not only PHA composition and molecular weight but also surface microstructure and porosity of the polymer films. The new strain can be recommended as a promising P(3HB) producer from palm oil, oleic acid, and sugars (fructose and glucose) and as a producer of P(3HB-co-3HV) from oleic acid and P(3HB-co-3HV-co-3HHx) from palm oil.https://www.mdpi.com/2073-4360/13/18/3142<i>Cupriavidus necator</i> IBP/SFU-1cell growth and PHA synthesisvarious carbon sourcesPHA composition and propertiespolymer films |
| spellingShingle | Natalia O. Zhila Kristina Yu. Sapozhnikova Evgeniy G. Kiselev Alexander D. Vasiliev Ivan V. Nemtsev Ekaterina I. Shishatskaya Tatiana G. Volova Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, <i>Cupriavidus necator</i> IBP/SFU-1, from Various Carbon Sources Polymers <i>Cupriavidus necator</i> IBP/SFU-1 cell growth and PHA synthesis various carbon sources PHA composition and properties polymer films |
| title | Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, <i>Cupriavidus necator</i> IBP/SFU-1, from Various Carbon Sources |
| title_full | Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, <i>Cupriavidus necator</i> IBP/SFU-1, from Various Carbon Sources |
| title_fullStr | Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, <i>Cupriavidus necator</i> IBP/SFU-1, from Various Carbon Sources |
| title_full_unstemmed | Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, <i>Cupriavidus necator</i> IBP/SFU-1, from Various Carbon Sources |
| title_short | Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, <i>Cupriavidus necator</i> IBP/SFU-1, from Various Carbon Sources |
| title_sort | properties of degradable polyhydroxyalkanoates phas synthesized by a new strain i cupriavidus necator i ibp sfu 1 from various carbon sources |
| topic | <i>Cupriavidus necator</i> IBP/SFU-1 cell growth and PHA synthesis various carbon sources PHA composition and properties polymer films |
| url | https://www.mdpi.com/2073-4360/13/18/3142 |
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