Comparison of Colorimetric and Fluorometric Chemosensors for Protein Concentration Determination and Approaches for Estimation of Their Limits of Detection
Here, we present a direct comparison of different dyes and assays for the determination of protein concentrations. We compared the classical Bradford assay with two modern assays based on the fluorogenic dyes QuDye and ProteOrange and showed that the Bradford reagent achieved excellent results in th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/10/12/542 |
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| author | Anastasiya A. Mamaeva Vladimir I. Martynov Sergey M. Deyev Alexey A. Pakhomov |
| author_facet | Anastasiya A. Mamaeva Vladimir I. Martynov Sergey M. Deyev Alexey A. Pakhomov |
| author_sort | Anastasiya A. Mamaeva |
| collection | DOAJ |
| description | Here, we present a direct comparison of different dyes and assays for the determination of protein concentrations. We compared the classical Bradford assay with two modern assays based on the fluorogenic dyes QuDye and ProteOrange and showed that the Bradford reagent achieved excellent results in the determination of protein concentrations as compared with more modern rivals. We also showed that standard approaches for determining the limit of detection (LoD) and limit of quantification (LoQ) may not work correctly with the tested dyes. We proposed a new approach that extends the standard algorithm for LoD and LoQ determination. This approach works well with both classical colorimetric and fluorogenic dyes, as well as with nontrivial fluorescent probes. |
| first_indexed | 2024-03-09T17:12:46Z |
| format | Article |
| id | doaj.art-db402ea68b2a471fbe8b8840bbdab3dd |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-09T17:12:46Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-db402ea68b2a471fbe8b8840bbdab3dd2023-11-24T13:59:45ZengMDPI AGChemosensors2227-90402022-12-01101254210.3390/chemosensors10120542Comparison of Colorimetric and Fluorometric Chemosensors for Protein Concentration Determination and Approaches for Estimation of Their Limits of DetectionAnastasiya A. Mamaeva0Vladimir I. Martynov1Sergey M. Deyev2Alexey A. Pakhomov3M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaM.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaM.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaM.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaHere, we present a direct comparison of different dyes and assays for the determination of protein concentrations. We compared the classical Bradford assay with two modern assays based on the fluorogenic dyes QuDye and ProteOrange and showed that the Bradford reagent achieved excellent results in the determination of protein concentrations as compared with more modern rivals. We also showed that standard approaches for determining the limit of detection (LoD) and limit of quantification (LoQ) may not work correctly with the tested dyes. We proposed a new approach that extends the standard algorithm for LoD and LoQ determination. This approach works well with both classical colorimetric and fluorogenic dyes, as well as with nontrivial fluorescent probes.https://www.mdpi.com/2227-9040/10/12/542Bradford reagentProteOrangeQuDyefluorescent dyeprotein assayLoD |
| spellingShingle | Anastasiya A. Mamaeva Vladimir I. Martynov Sergey M. Deyev Alexey A. Pakhomov Comparison of Colorimetric and Fluorometric Chemosensors for Protein Concentration Determination and Approaches for Estimation of Their Limits of Detection Chemosensors Bradford reagent ProteOrange QuDye fluorescent dye protein assay LoD |
| title | Comparison of Colorimetric and Fluorometric Chemosensors for Protein Concentration Determination and Approaches for Estimation of Their Limits of Detection |
| title_full | Comparison of Colorimetric and Fluorometric Chemosensors for Protein Concentration Determination and Approaches for Estimation of Their Limits of Detection |
| title_fullStr | Comparison of Colorimetric and Fluorometric Chemosensors for Protein Concentration Determination and Approaches for Estimation of Their Limits of Detection |
| title_full_unstemmed | Comparison of Colorimetric and Fluorometric Chemosensors for Protein Concentration Determination and Approaches for Estimation of Their Limits of Detection |
| title_short | Comparison of Colorimetric and Fluorometric Chemosensors for Protein Concentration Determination and Approaches for Estimation of Their Limits of Detection |
| title_sort | comparison of colorimetric and fluorometric chemosensors for protein concentration determination and approaches for estimation of their limits of detection |
| topic | Bradford reagent ProteOrange QuDye fluorescent dye protein assay LoD |
| url | https://www.mdpi.com/2227-9040/10/12/542 |
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