Comparison of Colorimetric and Fluorometric Chemosensors for Protein Concentration Determination and Approaches for Estimation of Their Limits of Detection

Comparison of Colorimetric and Fluorometric Chemosensors for Protein Concentration Determination and Approaches for Estimation of Their Limits of Detection

Here, we present a direct comparison of different dyes and assays for the determination of protein concentrations. We compared the classical Bradford assay with two modern assays based on the fluorogenic dyes QuDye and ProteOrange and showed that the Bradford reagent achieved excellent results in th...

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Bibliographic Details
Main Authors: Anastasiya A. Mamaeva, Vladimir I. Martynov, Sergey M. Deyev, Alexey A. Pakhomov
Format: Article
Language:English
Published: MDPI AG 2022-12-01
Series:Chemosensors
Subjects:
Bradford reagent
ProteOrange
QuDye
fluorescent dye
protein assay
LoD
Online Access:https://www.mdpi.com/2227-9040/10/12/542

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