Antimicrobial Activity of Propolis from the Brazilian Stingless Bees <i>Melipona quadrifasciata anthidioides</i> and <i>Scaptotrigona depilis</i> (Hymenoptera, Apidae, Meliponini)
<i>Melipona quadrifasciata anthidioides</i> and <i>Scaptotrigona depilis</i> are species of stingless bees capable of producing propolis, which has considerable bioprospecting potential. In this context, the objective of this study was to determine the chemical compositions a...
| Principais autores: | , , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | English |
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MDPI AG
2022-12-01
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| coleção: | Microorganisms |
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| Acesso em linha: | https://www.mdpi.com/2076-2607/11/1/68 |
| _version_ | 1827623172187357184 |
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| author | Jaqueline Ferreira Campos Thaliny Bonamigo Paola dos Santos da Rocha Vanessa Marina Branco Paula Uilson Pereira dos Santos José Benedito Perrella Balestieri Denise Brentan Silva Carlos Alexandre Carollo Leticia M. Estevinho Kely de Picoli Souza Edson Lucas dos Santos |
| author_facet | Jaqueline Ferreira Campos Thaliny Bonamigo Paola dos Santos da Rocha Vanessa Marina Branco Paula Uilson Pereira dos Santos José Benedito Perrella Balestieri Denise Brentan Silva Carlos Alexandre Carollo Leticia M. Estevinho Kely de Picoli Souza Edson Lucas dos Santos |
| author_sort | Jaqueline Ferreira Campos |
| collection | DOAJ |
| description | <i>Melipona quadrifasciata anthidioides</i> and <i>Scaptotrigona depilis</i> are species of stingless bees capable of producing propolis, which has considerable bioprospecting potential. In this context, the objective of this study was to determine the chemical compositions and evaluate the antimicrobial activity of propolis produced by <i>M. q. anthidioides</i> and <i>S. depilis</i>. The ethanolic extracts of propolis of <i>M. q. anthidioides</i> (EEP-M) and <i>S. depilis</i> (EEP-S) were prepared, and their chemical constituents were characterized by HPLC-ESI-MS. The antimicrobial activity was evaluated against bacteria and fungi, isolated from reference strains and hospital origin resistant to the action of antibiotics. From EEP-M, phenolic compounds were annotated, including gallic acid, ellagic acid, and flavonoids, as well as diterpenes and triterpenes. EEP-S showed mainly triterpene in its chemical composition. Both extracts inhibited the growth of medically relevant bacteria and fungi, including hospital-acquired and antimicrobial-resistant. In general, EEP-S showed better antimicrobial activity compared to EEP-M. The MIC of EEP-S against vancomycin-resistant <i>Enterococcus faecalis</i> was 3.50 mg/mL, while the MIC of EEP-M was 5.33 ± 0.16 mg/mL. In conclusion, this study shows that propolis produced by <i>M. q. anthidioides</i> and <i>S. depilis</i> has the potential to be used for the prevention or treatment of microbial infections. |
| first_indexed | 2024-03-09T11:39:38Z |
| format | Article |
| id | doaj.art-db43c06eaecd4ba786d3b7ba2ee7e97c |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T11:39:38Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-db43c06eaecd4ba786d3b7ba2ee7e97c2023-11-30T23:35:56ZengMDPI AGMicroorganisms2076-26072022-12-011116810.3390/microorganisms11010068Antimicrobial Activity of Propolis from the Brazilian Stingless Bees <i>Melipona quadrifasciata anthidioides</i> and <i>Scaptotrigona depilis</i> (Hymenoptera, Apidae, Meliponini)Jaqueline Ferreira Campos0Thaliny Bonamigo1Paola dos Santos da Rocha2Vanessa Marina Branco Paula3Uilson Pereira dos Santos4José Benedito Perrella Balestieri5Denise Brentan Silva6Carlos Alexandre Carollo7Leticia M. Estevinho8Kely de Picoli Souza9Edson Lucas dos Santos10Research Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados 79804-970, BrazilResearch Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados 79804-970, BrazilResearch Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados 79804-970, BrazilCIMO-Mountain Research Center, Department of Biology and Biotechnology, Polytechnic Institute of Bragança, Campus Santa Apolónia, Agricultural College of Bragança, Bragança 5301-855, PortugalResearch Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados 79804-970, BrazilResearch Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados 79804-970, BrazilLaboratory of Natural Products and Mass Spectrometry, Federal University of Mato Grosso do Sul, Cidade Universitária, Campo Grande 79070-900, BrazilLaboratory of Natural Products and Mass Spectrometry, Federal University of Mato Grosso do Sul, Cidade Universitária, Campo Grande 79070-900, BrazilCIMO-Mountain Research Center, Department of Biology and Biotechnology, Polytechnic Institute of Bragança, Campus Santa Apolónia, Agricultural College of Bragança, Bragança 5301-855, PortugalResearch Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados 79804-970, BrazilResearch Group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Dourados 79804-970, Brazil<i>Melipona quadrifasciata anthidioides</i> and <i>Scaptotrigona depilis</i> are species of stingless bees capable of producing propolis, which has considerable bioprospecting potential. In this context, the objective of this study was to determine the chemical compositions and evaluate the antimicrobial activity of propolis produced by <i>M. q. anthidioides</i> and <i>S. depilis</i>. The ethanolic extracts of propolis of <i>M. q. anthidioides</i> (EEP-M) and <i>S. depilis</i> (EEP-S) were prepared, and their chemical constituents were characterized by HPLC-ESI-MS. The antimicrobial activity was evaluated against bacteria and fungi, isolated from reference strains and hospital origin resistant to the action of antibiotics. From EEP-M, phenolic compounds were annotated, including gallic acid, ellagic acid, and flavonoids, as well as diterpenes and triterpenes. EEP-S showed mainly triterpene in its chemical composition. Both extracts inhibited the growth of medically relevant bacteria and fungi, including hospital-acquired and antimicrobial-resistant. In general, EEP-S showed better antimicrobial activity compared to EEP-M. The MIC of EEP-S against vancomycin-resistant <i>Enterococcus faecalis</i> was 3.50 mg/mL, while the MIC of EEP-M was 5.33 ± 0.16 mg/mL. In conclusion, this study shows that propolis produced by <i>M. q. anthidioides</i> and <i>S. depilis</i> has the potential to be used for the prevention or treatment of microbial infections.https://www.mdpi.com/2076-2607/11/1/68natural productsMeliponiniHPLC-ESI-MSresistant microorganisms |
| spellingShingle | Jaqueline Ferreira Campos Thaliny Bonamigo Paola dos Santos da Rocha Vanessa Marina Branco Paula Uilson Pereira dos Santos José Benedito Perrella Balestieri Denise Brentan Silva Carlos Alexandre Carollo Leticia M. Estevinho Kely de Picoli Souza Edson Lucas dos Santos Antimicrobial Activity of Propolis from the Brazilian Stingless Bees <i>Melipona quadrifasciata anthidioides</i> and <i>Scaptotrigona depilis</i> (Hymenoptera, Apidae, Meliponini) Microorganisms natural products Meliponini HPLC-ESI-MS resistant microorganisms |
| title | Antimicrobial Activity of Propolis from the Brazilian Stingless Bees <i>Melipona quadrifasciata anthidioides</i> and <i>Scaptotrigona depilis</i> (Hymenoptera, Apidae, Meliponini) |
| title_full | Antimicrobial Activity of Propolis from the Brazilian Stingless Bees <i>Melipona quadrifasciata anthidioides</i> and <i>Scaptotrigona depilis</i> (Hymenoptera, Apidae, Meliponini) |
| title_fullStr | Antimicrobial Activity of Propolis from the Brazilian Stingless Bees <i>Melipona quadrifasciata anthidioides</i> and <i>Scaptotrigona depilis</i> (Hymenoptera, Apidae, Meliponini) |
| title_full_unstemmed | Antimicrobial Activity of Propolis from the Brazilian Stingless Bees <i>Melipona quadrifasciata anthidioides</i> and <i>Scaptotrigona depilis</i> (Hymenoptera, Apidae, Meliponini) |
| title_short | Antimicrobial Activity of Propolis from the Brazilian Stingless Bees <i>Melipona quadrifasciata anthidioides</i> and <i>Scaptotrigona depilis</i> (Hymenoptera, Apidae, Meliponini) |
| title_sort | antimicrobial activity of propolis from the brazilian stingless bees i melipona quadrifasciata anthidioides i and i scaptotrigona depilis i hymenoptera apidae meliponini |
| topic | natural products Meliponini HPLC-ESI-MS resistant microorganisms |
| url | https://www.mdpi.com/2076-2607/11/1/68 |
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