Cellular antioxidant activities of polyphenols isolated from Eucalyptus leaves (Eucalyptus grandis × Eucalyptus urophylla GL9)
An activity-guided isolation process was performed to identify the antioxidant constituents of Eucalyptus leaves (Eucalyptus grandis × Eucalyptus urophylla GL9). The ethyl acetate extract showed the highest activity in scavenging DPPH radical among five different fractions extracted from 70% aqueous...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-03-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S175646461300296X |
| _version_ | 1819293771584503808 |
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| author | Yunjiao Chen Junjiang Wang Yangwen Ou Hongzhang Chen Suyao Xiao Guo Liu Yong Cao Qingrong Huang |
| author_facet | Yunjiao Chen Junjiang Wang Yangwen Ou Hongzhang Chen Suyao Xiao Guo Liu Yong Cao Qingrong Huang |
| author_sort | Yunjiao Chen |
| collection | DOAJ |
| description | An activity-guided isolation process was performed to identify the antioxidant constituents of Eucalyptus leaves (Eucalyptus grandis × Eucalyptus urophylla GL9). The ethyl acetate extract showed the highest activity in scavenging DPPH radical among five different fractions extracted from 70% aqueous ethanol extract of Eucalyptus leaves. Nine known compounds possessing radical scavenging activity, which included gallic acid (1), quercetin-3-O-β-d-glucuronide (Q-3-G-A) (2), ethyl gallate (3), tellimagrandin II(4), chlorogenic acid (5), 3-O-galloyl-4,6-O-[(S)-hexahydroxydiphenoyl]-d-glucose (gemin D) (6), pedunculagin (7), tellimagrandin I (8), and 1,2,3,4,6-penta-O-galloyl-β-d-glucose (PGG) (9) were isolated and identified from the ethyl acetate extract. Our results indicated that ethyl acetate extract and hydrolysable tannins among nine compounds exhibited great antioxidant activity in both chemical-based (e.g. DPPH and ABTS assays) and cellular-based (i.e., Hep G2) antioxidant assays, and PGG showed the highest cellular antioxidant activity. This study suggested that ethyl acetate extract and the hydrolysable tannins in Eucalyptus leaves would be promising natural antioxidants in pharmaceutical, feed and food supplement industries. |
| first_indexed | 2024-12-24T04:15:43Z |
| format | Article |
| id | doaj.art-db43df21532f4f078c3d3be15bafbd98 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-24T04:15:43Z |
| publishDate | 2014-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-db43df21532f4f078c3d3be15bafbd982022-12-21T17:15:57ZengElsevierJournal of Functional Foods1756-46462014-03-017737745Cellular antioxidant activities of polyphenols isolated from Eucalyptus leaves (Eucalyptus grandis × Eucalyptus urophylla GL9)Yunjiao Chen0Junjiang Wang1Yangwen Ou2Hongzhang Chen3Suyao Xiao4Guo Liu5Yong Cao6Qingrong Huang7Department of Food Science, College of Food Science, South China Agricultural University, Guangzhou 510640, China; Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, NJ 08901, USADepartment of Food Science, College of Food Science, South China Agricultural University, Guangzhou 510640, ChinaDepartment of Food Science, College of Food Science, South China Agricultural University, Guangzhou 510640, China; School of Pharmacy, Hunan University of Traditional Chinese Medicine, Changsha 410208, ChinaDepartment of Food Science, College of Food Science, South China Agricultural University, Guangzhou 510640, ChinaDepartment of Food Science, College of Food Science, South China Agricultural University, Guangzhou 510640, ChinaDepartment of Food Science, College of Food Science, South China Agricultural University, Guangzhou 510640, ChinaDepartment of Food Science, College of Food Science, South China Agricultural University, Guangzhou 510640, China; Corresponding authors. Tel./fax: +86 20 85286234 (Y. Cao). Tel.: +1 848 932 5514; fax: +1 732 932 6776 (Q. Huang).Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, NJ 08901, USA; Corresponding authors. Tel./fax: +86 20 85286234 (Y. Cao). Tel.: +1 848 932 5514; fax: +1 732 932 6776 (Q. Huang).An activity-guided isolation process was performed to identify the antioxidant constituents of Eucalyptus leaves (Eucalyptus grandis × Eucalyptus urophylla GL9). The ethyl acetate extract showed the highest activity in scavenging DPPH radical among five different fractions extracted from 70% aqueous ethanol extract of Eucalyptus leaves. Nine known compounds possessing radical scavenging activity, which included gallic acid (1), quercetin-3-O-β-d-glucuronide (Q-3-G-A) (2), ethyl gallate (3), tellimagrandin II(4), chlorogenic acid (5), 3-O-galloyl-4,6-O-[(S)-hexahydroxydiphenoyl]-d-glucose (gemin D) (6), pedunculagin (7), tellimagrandin I (8), and 1,2,3,4,6-penta-O-galloyl-β-d-glucose (PGG) (9) were isolated and identified from the ethyl acetate extract. Our results indicated that ethyl acetate extract and hydrolysable tannins among nine compounds exhibited great antioxidant activity in both chemical-based (e.g. DPPH and ABTS assays) and cellular-based (i.e., Hep G2) antioxidant assays, and PGG showed the highest cellular antioxidant activity. This study suggested that ethyl acetate extract and the hydrolysable tannins in Eucalyptus leaves would be promising natural antioxidants in pharmaceutical, feed and food supplement industries.http://www.sciencedirect.com/science/article/pii/S175646461300296XEucalyptus leavesCellular antioxidant activityHydrolysable tannins |
| spellingShingle | Yunjiao Chen Junjiang Wang Yangwen Ou Hongzhang Chen Suyao Xiao Guo Liu Yong Cao Qingrong Huang Cellular antioxidant activities of polyphenols isolated from Eucalyptus leaves (Eucalyptus grandis × Eucalyptus urophylla GL9) Journal of Functional Foods Eucalyptus leaves Cellular antioxidant activity Hydrolysable tannins |
| title | Cellular antioxidant activities of polyphenols isolated from Eucalyptus leaves (Eucalyptus grandis × Eucalyptus urophylla GL9) |
| title_full | Cellular antioxidant activities of polyphenols isolated from Eucalyptus leaves (Eucalyptus grandis × Eucalyptus urophylla GL9) |
| title_fullStr | Cellular antioxidant activities of polyphenols isolated from Eucalyptus leaves (Eucalyptus grandis × Eucalyptus urophylla GL9) |
| title_full_unstemmed | Cellular antioxidant activities of polyphenols isolated from Eucalyptus leaves (Eucalyptus grandis × Eucalyptus urophylla GL9) |
| title_short | Cellular antioxidant activities of polyphenols isolated from Eucalyptus leaves (Eucalyptus grandis × Eucalyptus urophylla GL9) |
| title_sort | cellular antioxidant activities of polyphenols isolated from eucalyptus leaves eucalyptus grandis eucalyptus urophylla gl9 |
| topic | Eucalyptus leaves Cellular antioxidant activity Hydrolysable tannins |
| url | http://www.sciencedirect.com/science/article/pii/S175646461300296X |
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