Effects of α-Lipoic Acid Supplementation on Growth Performance, Liver Histology, Antioxidant and Related Genes Expression of Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂)
This study aimed to assess the impact of α-lipoic acid on the growth performance, antioxidant capacity and immunity in hybrid groupers (♀ <i>Epinephelus fuscoguttatus</i> × ♂ <i>E. lanceolatus</i>) fed with a high-lipid diet. Groupers (8.97 ± 0.01 g) were fed six different di...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/13/1/88 |
| _version_ | 1797344715362795520 |
|---|---|
| author | Weibin Huang Tao Li Wenshan Cai Hengyang Song Hao Liu Beiping Tan Shuang Zhang Menglong Zhou Yuanzhi Yang Xiaohui Dong |
| author_facet | Weibin Huang Tao Li Wenshan Cai Hengyang Song Hao Liu Beiping Tan Shuang Zhang Menglong Zhou Yuanzhi Yang Xiaohui Dong |
| author_sort | Weibin Huang |
| collection | DOAJ |
| description | This study aimed to assess the impact of α-lipoic acid on the growth performance, antioxidant capacity and immunity in hybrid groupers (♀ <i>Epinephelus fuscoguttatus</i> × ♂ <i>E. lanceolatus</i>) fed with a high-lipid diet. Groupers (8.97 ± 0.01 g) were fed six different diets, with α-lipoic acid content in diets being 0, 400, 800, 1200, 1600, and 2000 mg/kg, named S1, S2, S3, S4, S5, and S6, respectively. The results show that the addition of 2000 mg/kg α-lipoic acid in the diet inhibited the growth, weight gain rate (WGR), and specific growth rate (SGR), which were significantly lower than other groups. In serum, catalase (CAT) and superoxide dismutase (SOD) were significantly higher in the S5 group than in the S1 group. In the liver, CAT, SOD and total antioxidative capacity (T-AOC) levels were significantly increased in α-lipoic acid supplemented groups. α-lipoic acid significantly upregulated liver antioxidant genes <i>sod</i> and <i>cat</i>, anti-inflammatory factor interleukin 10 (<i>il10</i>) and transforming growth factor β (<i>tgfβ</i>) mRNA levels. Conclusion: the addition of 2000 mg/kg of α-lipoic acid inhibits the growth of hybrid groupers. In addition, 400–800 mg/kg α-lipoic acid contents improve the antioxidant capacity of groupers and have a protective effect against high-lipid-diet-induced liver oxidative damage. |
| first_indexed | 2024-03-08T11:06:45Z |
| format | Article |
| id | doaj.art-db44360904b14e3b91412bd034ef0df4 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-08T11:06:45Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-db44360904b14e3b91412bd034ef0df42024-01-26T14:41:24ZengMDPI AGAntioxidants2076-39212024-01-011318810.3390/antiox13010088Effects of α-Lipoic Acid Supplementation on Growth Performance, Liver Histology, Antioxidant and Related Genes Expression of Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂)Weibin Huang0Tao Li1Wenshan Cai2Hengyang Song3Hao Liu4Beiping Tan5Shuang Zhang6Menglong Zhou7Yuanzhi Yang8Xiaohui Dong9Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaLaboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaLaboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaLaboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaLaboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaLaboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaLaboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaLaboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaLaboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaLaboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaThis study aimed to assess the impact of α-lipoic acid on the growth performance, antioxidant capacity and immunity in hybrid groupers (♀ <i>Epinephelus fuscoguttatus</i> × ♂ <i>E. lanceolatus</i>) fed with a high-lipid diet. Groupers (8.97 ± 0.01 g) were fed six different diets, with α-lipoic acid content in diets being 0, 400, 800, 1200, 1600, and 2000 mg/kg, named S1, S2, S3, S4, S5, and S6, respectively. The results show that the addition of 2000 mg/kg α-lipoic acid in the diet inhibited the growth, weight gain rate (WGR), and specific growth rate (SGR), which were significantly lower than other groups. In serum, catalase (CAT) and superoxide dismutase (SOD) were significantly higher in the S5 group than in the S1 group. In the liver, CAT, SOD and total antioxidative capacity (T-AOC) levels were significantly increased in α-lipoic acid supplemented groups. α-lipoic acid significantly upregulated liver antioxidant genes <i>sod</i> and <i>cat</i>, anti-inflammatory factor interleukin 10 (<i>il10</i>) and transforming growth factor β (<i>tgfβ</i>) mRNA levels. Conclusion: the addition of 2000 mg/kg of α-lipoic acid inhibits the growth of hybrid groupers. In addition, 400–800 mg/kg α-lipoic acid contents improve the antioxidant capacity of groupers and have a protective effect against high-lipid-diet-induced liver oxidative damage.https://www.mdpi.com/2076-3921/13/1/88high-lipid diethybrid grouperantioxidantimmunityα-lipoic acid |
| spellingShingle | Weibin Huang Tao Li Wenshan Cai Hengyang Song Hao Liu Beiping Tan Shuang Zhang Menglong Zhou Yuanzhi Yang Xiaohui Dong Effects of α-Lipoic Acid Supplementation on Growth Performance, Liver Histology, Antioxidant and Related Genes Expression of Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂) Antioxidants high-lipid diet hybrid grouper antioxidant immunity α-lipoic acid |
| title | Effects of α-Lipoic Acid Supplementation on Growth Performance, Liver Histology, Antioxidant and Related Genes Expression of Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂) |
| title_full | Effects of α-Lipoic Acid Supplementation on Growth Performance, Liver Histology, Antioxidant and Related Genes Expression of Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂) |
| title_fullStr | Effects of α-Lipoic Acid Supplementation on Growth Performance, Liver Histology, Antioxidant and Related Genes Expression of Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂) |
| title_full_unstemmed | Effects of α-Lipoic Acid Supplementation on Growth Performance, Liver Histology, Antioxidant and Related Genes Expression of Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂) |
| title_short | Effects of α-Lipoic Acid Supplementation on Growth Performance, Liver Histology, Antioxidant and Related Genes Expression of Hybrid Grouper (<i>Epinephelus fuscoguttatus</i> ♀ × <i>E. lanceolatus</i> ♂) |
| title_sort | effects of α lipoic acid supplementation on growth performance liver histology antioxidant and related genes expression of hybrid grouper i epinephelus fuscoguttatus i ♀ i e lanceolatus i ♂ |
| topic | high-lipid diet hybrid grouper antioxidant immunity α-lipoic acid |
| url | https://www.mdpi.com/2076-3921/13/1/88 |
| work_keys_str_mv | AT weibinhuang effectsofalipoicacidsupplementationongrowthperformanceliverhistologyantioxidantandrelatedgenesexpressionofhybridgrouperiepinephelusfuscoguttatusiielanceolatusi AT taoli effectsofalipoicacidsupplementationongrowthperformanceliverhistologyantioxidantandrelatedgenesexpressionofhybridgrouperiepinephelusfuscoguttatusiielanceolatusi AT wenshancai effectsofalipoicacidsupplementationongrowthperformanceliverhistologyantioxidantandrelatedgenesexpressionofhybridgrouperiepinephelusfuscoguttatusiielanceolatusi AT hengyangsong effectsofalipoicacidsupplementationongrowthperformanceliverhistologyantioxidantandrelatedgenesexpressionofhybridgrouperiepinephelusfuscoguttatusiielanceolatusi AT haoliu effectsofalipoicacidsupplementationongrowthperformanceliverhistologyantioxidantandrelatedgenesexpressionofhybridgrouperiepinephelusfuscoguttatusiielanceolatusi AT beipingtan effectsofalipoicacidsupplementationongrowthperformanceliverhistologyantioxidantandrelatedgenesexpressionofhybridgrouperiepinephelusfuscoguttatusiielanceolatusi AT shuangzhang effectsofalipoicacidsupplementationongrowthperformanceliverhistologyantioxidantandrelatedgenesexpressionofhybridgrouperiepinephelusfuscoguttatusiielanceolatusi AT menglongzhou effectsofalipoicacidsupplementationongrowthperformanceliverhistologyantioxidantandrelatedgenesexpressionofhybridgrouperiepinephelusfuscoguttatusiielanceolatusi AT yuanzhiyang effectsofalipoicacidsupplementationongrowthperformanceliverhistologyantioxidantandrelatedgenesexpressionofhybridgrouperiepinephelusfuscoguttatusiielanceolatusi AT xiaohuidong effectsofalipoicacidsupplementationongrowthperformanceliverhistologyantioxidantandrelatedgenesexpressionofhybridgrouperiepinephelusfuscoguttatusiielanceolatusi |