A Multienzyme Reaction-Mediated Electrochemical Biosensor for Sensitive Detection of Organophosphorus Pesticides
Ethephon (ETH), a commonly employed growth regulator, poses potential health risks due to its residue in fruits and vegetables, leading to both acute and subchronic toxicity. However, the detection accuracy of ETH is compromised by the color effects of the samples during the detection process. In th...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/14/2/62 |
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| author | Chengzhen Ji Xuemei Tang Ruiming Wen Chengdong Xu Jing Wei Bingjun Han Long Wu |
| author_facet | Chengzhen Ji Xuemei Tang Ruiming Wen Chengdong Xu Jing Wei Bingjun Han Long Wu |
| author_sort | Chengzhen Ji |
| collection | DOAJ |
| description | Ethephon (ETH), a commonly employed growth regulator, poses potential health risks due to its residue in fruits and vegetables, leading to both acute and subchronic toxicity. However, the detection accuracy of ETH is compromised by the color effects of the samples during the detection process. In this work, a multienzyme reaction-mediated electrochemical biosensor (MRMEC) was developed for the sensitive, rapid, and color-interference-resistant determination of ETH. Nanozymes Fe<sub>3</sub>O<sub>4</sub>@Au–Pt and graphene nanocomplexes (GN–Au NPs) were prepared as catalysts and signal amplifiers for MRMEC. Acetylcholinesterase (AChE), acetylcholine (ACh), and choline oxidase (CHOx) form a cascade enzyme reaction to produce H<sub>2</sub>O<sub>2</sub> in an electrolytic cell. Fe<sub>3</sub>O<sub>4</sub>@Au–Pt has excellent peroxidase-like activity and can catalyze the oxidation of 3,3′,5,5′-tetramethvlbenzidine (TMB) in the presence of H<sub>2</sub>O<sub>2</sub>, resulting in a decrease in the characteristic peak current of TMB. Based on the inhibitory effect of ETH on AChE, the differential pulse voltammetry (DPV) current signal of TMB was used to detect ETH, offering the limit of detection (LOD) of 2.01 nmol L<sup>−1</sup>. The MRMEC method effectively analyzed ETH levels in mangoes, showing satisfactory precision (coefficient of variations, 2.88–15.97%) and recovery rate (92.18–110.72%). This biosensor holds promise for detecting various organophosphorus pesticides in food samples. |
| first_indexed | 2024-03-07T22:41:05Z |
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| language | English |
| last_indexed | 2024-03-07T22:41:05Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
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| series | Biosensors |
| spelling | doaj.art-db458c05eeae4210ab101e1fac0fcba22024-02-23T15:09:29ZengMDPI AGBiosensors2079-63742024-01-011426210.3390/bios14020062A Multienzyme Reaction-Mediated Electrochemical Biosensor for Sensitive Detection of Organophosphorus PesticidesChengzhen Ji0Xuemei Tang1Ruiming Wen2Chengdong Xu3Jing Wei4Bingjun Han5Long Wu6Hainan Engineering Research Center of Aquatic Resources Efficient Utilization in South China Sea, Key Laboratory of Seafood Processing of Haikou, School of Food Science and Engineering, Hainan University, Haikou 570228, ChinaHainan Engineering Research Center of Aquatic Resources Efficient Utilization in South China Sea, Key Laboratory of Seafood Processing of Haikou, School of Food Science and Engineering, Hainan University, Haikou 570228, ChinaHainan Engineering Research Center of Aquatic Resources Efficient Utilization in South China Sea, Key Laboratory of Seafood Processing of Haikou, School of Food Science and Engineering, Hainan University, Haikou 570228, ChinaHainan Engineering Research Center of Aquatic Resources Efficient Utilization in South China Sea, Key Laboratory of Seafood Processing of Haikou, School of Food Science and Engineering, Hainan University, Haikou 570228, ChinaKey Laboratory of Tropical Fruits and Vegetables Quality and Safety for State Market Regulation, Hainan Institute for Food Control, Haikou 570314, ChinaHainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables, Analysis and Test Center, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaHainan Engineering Research Center of Aquatic Resources Efficient Utilization in South China Sea, Key Laboratory of Seafood Processing of Haikou, School of Food Science and Engineering, Hainan University, Haikou 570228, ChinaEthephon (ETH), a commonly employed growth regulator, poses potential health risks due to its residue in fruits and vegetables, leading to both acute and subchronic toxicity. However, the detection accuracy of ETH is compromised by the color effects of the samples during the detection process. In this work, a multienzyme reaction-mediated electrochemical biosensor (MRMEC) was developed for the sensitive, rapid, and color-interference-resistant determination of ETH. Nanozymes Fe<sub>3</sub>O<sub>4</sub>@Au–Pt and graphene nanocomplexes (GN–Au NPs) were prepared as catalysts and signal amplifiers for MRMEC. Acetylcholinesterase (AChE), acetylcholine (ACh), and choline oxidase (CHOx) form a cascade enzyme reaction to produce H<sub>2</sub>O<sub>2</sub> in an electrolytic cell. Fe<sub>3</sub>O<sub>4</sub>@Au–Pt has excellent peroxidase-like activity and can catalyze the oxidation of 3,3′,5,5′-tetramethvlbenzidine (TMB) in the presence of H<sub>2</sub>O<sub>2</sub>, resulting in a decrease in the characteristic peak current of TMB. Based on the inhibitory effect of ETH on AChE, the differential pulse voltammetry (DPV) current signal of TMB was used to detect ETH, offering the limit of detection (LOD) of 2.01 nmol L<sup>−1</sup>. The MRMEC method effectively analyzed ETH levels in mangoes, showing satisfactory precision (coefficient of variations, 2.88–15.97%) and recovery rate (92.18–110.72%). This biosensor holds promise for detecting various organophosphorus pesticides in food samples.https://www.mdpi.com/2079-6374/14/2/62tropical fruitsorganophosphorus pesticidesnanozymesenzyme inhibitionfood safety |
| spellingShingle | Chengzhen Ji Xuemei Tang Ruiming Wen Chengdong Xu Jing Wei Bingjun Han Long Wu A Multienzyme Reaction-Mediated Electrochemical Biosensor for Sensitive Detection of Organophosphorus Pesticides Biosensors tropical fruits organophosphorus pesticides nanozymes enzyme inhibition food safety |
| title | A Multienzyme Reaction-Mediated Electrochemical Biosensor for Sensitive Detection of Organophosphorus Pesticides |
| title_full | A Multienzyme Reaction-Mediated Electrochemical Biosensor for Sensitive Detection of Organophosphorus Pesticides |
| title_fullStr | A Multienzyme Reaction-Mediated Electrochemical Biosensor for Sensitive Detection of Organophosphorus Pesticides |
| title_full_unstemmed | A Multienzyme Reaction-Mediated Electrochemical Biosensor for Sensitive Detection of Organophosphorus Pesticides |
| title_short | A Multienzyme Reaction-Mediated Electrochemical Biosensor for Sensitive Detection of Organophosphorus Pesticides |
| title_sort | multienzyme reaction mediated electrochemical biosensor for sensitive detection of organophosphorus pesticides |
| topic | tropical fruits organophosphorus pesticides nanozymes enzyme inhibition food safety |
| url | https://www.mdpi.com/2079-6374/14/2/62 |
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