Transcriptome alterations in spermatogonial stem cells exposed to bisphenol A
Owing to their self-renewal and differentiation abilities, spermatogonial stem cells (SSCs) are essential for maintaining male fertility and species preservation through spermatogenesis. With an increase in exposure to plasticizers, the risk of endocrine-disrupting chemicals exerting mimetic effects...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-03-01
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| Series: | Animal Cells and Systems |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19768354.2022.2061592 |
| _version_ | 1818481368469864448 |
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| author | Jin Seop Ahn Jong-Hyun Won Do-Young Kim Sang-Eun Jung Bang-Jin Kim Jun-Mo Kim Buom-Yong Ryu |
| author_facet | Jin Seop Ahn Jong-Hyun Won Do-Young Kim Sang-Eun Jung Bang-Jin Kim Jun-Mo Kim Buom-Yong Ryu |
| author_sort | Jin Seop Ahn |
| collection | DOAJ |
| description | Owing to their self-renewal and differentiation abilities, spermatogonial stem cells (SSCs) are essential for maintaining male fertility and species preservation through spermatogenesis. With an increase in exposure to plasticizers, the risk of endocrine-disrupting chemicals exerting mimetic effects on estrogen receptors, such as bisphenol A (BPA), has also increased. This has led to concerns regarding the preservation of male fertility. BPA impairs spermatogenesis and the maintenance of SSCs; however, the transcriptome differences caused by BPA in SSCs are poorly understood. Thus, this study aimed to investigate the transcriptome differences in SSCs exposed to BPA, using RNA sequencing (RNA-Seq) analysis. We found that cell proliferation and survival were suppressed by SSC exposure to BPA. Therefore, we investigated transcriptome differences through RNA-Seq, functional annotation, and gene set enrichment analysis. Our results showed repetitive and abundant terms related to two genes of lysosomal acidification and five genes of glycosaminoglycan degradation. Furthermore, we validated the transcriptome analyses by detecting mRNA and protein expression levels. The findings confirmed the discovery of differentially expressed genes (DEGs) and the mechanism of SSCs following exposure to BPA. Taken together, we expect that the identified DEGs and lysosomal mechanisms could provide new insights into the preservation of male fertility and related research. |
| first_indexed | 2024-12-10T11:33:57Z |
| format | Article |
| id | doaj.art-db469125b64f41faa98775588db5e569 |
| institution | Directory Open Access Journal |
| issn | 1976-8354 2151-2485 |
| language | English |
| last_indexed | 2024-12-10T11:33:57Z |
| publishDate | 2022-03-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Cells and Systems |
| spelling | doaj.art-db469125b64f41faa98775588db5e5692022-12-22T01:50:29ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852022-03-01262708310.1080/19768354.2022.2061592Transcriptome alterations in spermatogonial stem cells exposed to bisphenol AJin Seop Ahn0Jong-Hyun Won1Do-Young Kim2Sang-Eun Jung3Bang-Jin Kim4Jun-Mo Kim5Buom-Yong Ryu6Department of Animal Science & Technology, BET Research Institute, Chung-Ang University, Anseong-si, Republic of KoreaDepartment of Animal Science & Technology, BET Research Institute, Chung-Ang University, Anseong-si, Republic of KoreaDepartment of Animal Science & Technology, BET Research Institute, Chung-Ang University, Anseong-si, Republic of KoreaDepartment of Animal Science & Technology, BET Research Institute, Chung-Ang University, Anseong-si, Republic of KoreaDepartment of Cancer Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USADepartment of Animal Science & Technology, BET Research Institute, Chung-Ang University, Anseong-si, Republic of KoreaDepartment of Animal Science & Technology, BET Research Institute, Chung-Ang University, Anseong-si, Republic of KoreaOwing to their self-renewal and differentiation abilities, spermatogonial stem cells (SSCs) are essential for maintaining male fertility and species preservation through spermatogenesis. With an increase in exposure to plasticizers, the risk of endocrine-disrupting chemicals exerting mimetic effects on estrogen receptors, such as bisphenol A (BPA), has also increased. This has led to concerns regarding the preservation of male fertility. BPA impairs spermatogenesis and the maintenance of SSCs; however, the transcriptome differences caused by BPA in SSCs are poorly understood. Thus, this study aimed to investigate the transcriptome differences in SSCs exposed to BPA, using RNA sequencing (RNA-Seq) analysis. We found that cell proliferation and survival were suppressed by SSC exposure to BPA. Therefore, we investigated transcriptome differences through RNA-Seq, functional annotation, and gene set enrichment analysis. Our results showed repetitive and abundant terms related to two genes of lysosomal acidification and five genes of glycosaminoglycan degradation. Furthermore, we validated the transcriptome analyses by detecting mRNA and protein expression levels. The findings confirmed the discovery of differentially expressed genes (DEGs) and the mechanism of SSCs following exposure to BPA. Taken together, we expect that the identified DEGs and lysosomal mechanisms could provide new insights into the preservation of male fertility and related research.https://www.tandfonline.com/doi/10.1080/19768354.2022.2061592Bisphenol ARNA sequencingspermatogonial stem cellsautophagy |
| spellingShingle | Jin Seop Ahn Jong-Hyun Won Do-Young Kim Sang-Eun Jung Bang-Jin Kim Jun-Mo Kim Buom-Yong Ryu Transcriptome alterations in spermatogonial stem cells exposed to bisphenol A Animal Cells and Systems Bisphenol A RNA sequencing spermatogonial stem cells autophagy |
| title | Transcriptome alterations in spermatogonial stem cells exposed to bisphenol A |
| title_full | Transcriptome alterations in spermatogonial stem cells exposed to bisphenol A |
| title_fullStr | Transcriptome alterations in spermatogonial stem cells exposed to bisphenol A |
| title_full_unstemmed | Transcriptome alterations in spermatogonial stem cells exposed to bisphenol A |
| title_short | Transcriptome alterations in spermatogonial stem cells exposed to bisphenol A |
| title_sort | transcriptome alterations in spermatogonial stem cells exposed to bisphenol a |
| topic | Bisphenol A RNA sequencing spermatogonial stem cells autophagy |
| url | https://www.tandfonline.com/doi/10.1080/19768354.2022.2061592 |
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