<i>SiMYB19</i> from Foxtail Millet (<i>Setaria italica</i>) Confers Transgenic Rice Tolerance to High Salt Stress in the Field
Salt stress is a major threat to crop quality and yield. Most experiments on salt stress-related genes have been conducted at the laboratory or greenhouse scale. Consequently, there is a lack of research demonstrating the merit of exploring these genes in field crops. Here, we found that the R2R3-MY...
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MDPI AG
2022-01-01
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| Series: | International Journal of Molecular Sciences |
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| author | Chengjie Xu Mingzhao Luo Xianjun Sun Jiji Yan Huawei Shi Huishu Yan Rongyue Yan Shuguang Wang Wensi Tang Yongbin Zhou Chunxiao Wang Zhaoshi Xu Jun Chen Youzhi Ma Qiyan Jiang Ming Chen Daizhen Sun |
| author_facet | Chengjie Xu Mingzhao Luo Xianjun Sun Jiji Yan Huawei Shi Huishu Yan Rongyue Yan Shuguang Wang Wensi Tang Yongbin Zhou Chunxiao Wang Zhaoshi Xu Jun Chen Youzhi Ma Qiyan Jiang Ming Chen Daizhen Sun |
| author_sort | Chengjie Xu |
| collection | DOAJ |
| description | Salt stress is a major threat to crop quality and yield. Most experiments on salt stress-related genes have been conducted at the laboratory or greenhouse scale. Consequently, there is a lack of research demonstrating the merit of exploring these genes in field crops. Here, we found that the R2R3-MYB transcription factor <i>SiMYB19</i> from foxtail millet is expressed mainly in the roots and is induced by various abiotic stressors such as salt, drought, low nitrogen, and abscisic acid. <i>SiMYB19</i> is tentatively localized to the nucleus and activates transcription. It enhances salt tolerance in transgenic rice at the germination and seedling stages. <i>SiMYB19</i> overexpression increased shoot height, grain yield, and salt tolerance in field- and salt pond-grown transgenic rice. <i>SiMYB19</i> overexpression promotes abscisic acid (ABA) accumulation in transgenic rice and upregulates the ABA synthesis gene <i>OsNCED3</i> and the ABA signal transduction pathway-related genes <i>OsPK1</i> and <i>OsABF2</i>. Thus, <i>SiMYB19</i> improves salt tolerance in transgenic rice by regulating ABA synthesis and signal transduction. Using rice heterologous expression analysis, the present study introduced a novel candidate gene for improving salt tolerance and increasing yield in crops grown in saline-alkali soil. |
| first_indexed | 2024-03-10T01:19:27Z |
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| id | doaj.art-db471192dd1f496183879e7238fa4c4e |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:19:27Z |
| publishDate | 2022-01-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-db471192dd1f496183879e7238fa4c4e2023-11-23T14:03:45ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-01-0123275610.3390/ijms23020756<i>SiMYB19</i> from Foxtail Millet (<i>Setaria italica</i>) Confers Transgenic Rice Tolerance to High Salt Stress in the FieldChengjie Xu0Mingzhao Luo1Xianjun Sun2Jiji Yan3Huawei Shi4Huishu Yan5Rongyue Yan6Shuguang Wang7Wensi Tang8Yongbin Zhou9Chunxiao Wang10Zhaoshi Xu11Jun Chen12Youzhi Ma13Qiyan Jiang14Ming Chen15Daizhen Sun16Key Laboratory of Sustainable Dryland Agriculture, College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, ChinaInstitute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, ChinaInstitute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, ChinaCollege of Life Sciences, Shanxi Normal University, Taiyuan 030006, ChinaKey Laboratory of Sustainable Dryland Agriculture, College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, ChinaKey Laboratory of Sustainable Dryland Agriculture, College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, ChinaKey Laboratory of Sustainable Dryland Agriculture, College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, ChinaKey Laboratory of Sustainable Dryland Agriculture, College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, ChinaInstitute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, ChinaInstitute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, ChinaInstitute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, ChinaInstitute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, ChinaInstitute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, ChinaInstitute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, ChinaInstitute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, ChinaInstitute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, ChinaKey Laboratory of Sustainable Dryland Agriculture, College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, ChinaSalt stress is a major threat to crop quality and yield. Most experiments on salt stress-related genes have been conducted at the laboratory or greenhouse scale. Consequently, there is a lack of research demonstrating the merit of exploring these genes in field crops. Here, we found that the R2R3-MYB transcription factor <i>SiMYB19</i> from foxtail millet is expressed mainly in the roots and is induced by various abiotic stressors such as salt, drought, low nitrogen, and abscisic acid. <i>SiMYB19</i> is tentatively localized to the nucleus and activates transcription. It enhances salt tolerance in transgenic rice at the germination and seedling stages. <i>SiMYB19</i> overexpression increased shoot height, grain yield, and salt tolerance in field- and salt pond-grown transgenic rice. <i>SiMYB19</i> overexpression promotes abscisic acid (ABA) accumulation in transgenic rice and upregulates the ABA synthesis gene <i>OsNCED3</i> and the ABA signal transduction pathway-related genes <i>OsPK1</i> and <i>OsABF2</i>. Thus, <i>SiMYB19</i> improves salt tolerance in transgenic rice by regulating ABA synthesis and signal transduction. Using rice heterologous expression analysis, the present study introduced a novel candidate gene for improving salt tolerance and increasing yield in crops grown in saline-alkali soil.https://www.mdpi.com/1422-0067/23/2/756ABA pathwayfoxtail millethigh salt stressMYB transcription factor |
| spellingShingle | Chengjie Xu Mingzhao Luo Xianjun Sun Jiji Yan Huawei Shi Huishu Yan Rongyue Yan Shuguang Wang Wensi Tang Yongbin Zhou Chunxiao Wang Zhaoshi Xu Jun Chen Youzhi Ma Qiyan Jiang Ming Chen Daizhen Sun <i>SiMYB19</i> from Foxtail Millet (<i>Setaria italica</i>) Confers Transgenic Rice Tolerance to High Salt Stress in the Field International Journal of Molecular Sciences ABA pathway foxtail millet high salt stress MYB transcription factor |
| title | <i>SiMYB19</i> from Foxtail Millet (<i>Setaria italica</i>) Confers Transgenic Rice Tolerance to High Salt Stress in the Field |
| title_full | <i>SiMYB19</i> from Foxtail Millet (<i>Setaria italica</i>) Confers Transgenic Rice Tolerance to High Salt Stress in the Field |
| title_fullStr | <i>SiMYB19</i> from Foxtail Millet (<i>Setaria italica</i>) Confers Transgenic Rice Tolerance to High Salt Stress in the Field |
| title_full_unstemmed | <i>SiMYB19</i> from Foxtail Millet (<i>Setaria italica</i>) Confers Transgenic Rice Tolerance to High Salt Stress in the Field |
| title_short | <i>SiMYB19</i> from Foxtail Millet (<i>Setaria italica</i>) Confers Transgenic Rice Tolerance to High Salt Stress in the Field |
| title_sort | i simyb19 i from foxtail millet i setaria italica i confers transgenic rice tolerance to high salt stress in the field |
| topic | ABA pathway foxtail millet high salt stress MYB transcription factor |
| url | https://www.mdpi.com/1422-0067/23/2/756 |
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