Prevalence of specific human papillomavirus genotypes among Moroccan women with invasive cervical cancer
Aim: The aim of this study is to investigate the prevalence of human papillomavirus (HPV) genotypes in Moroccan women diagnosed with invasive cervical cancer and to assess the association between HPV infection and some socio-demographic characteristics and clinicopathological features. Methods: In t...
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| Format: | Article |
| Language: | English |
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Open Exploration Publishing Inc.
2024-02-01
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| Series: | Exploration of Medicine |
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| Online Access: | https://www.explorationpub.com/Journals/em/Article/1001207 |
| _version_ | 1797289834569531392 |
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| author | Kaoutar Anouar Tadlaoui Mustapha Benhessou Abdelilah Laraqui Lina Benfdil El Arbi Bouaiti Mohammed El Mzibri Moulay Mustapha Ennaji |
| author_facet | Kaoutar Anouar Tadlaoui Mustapha Benhessou Abdelilah Laraqui Lina Benfdil El Arbi Bouaiti Mohammed El Mzibri Moulay Mustapha Ennaji |
| author_sort | Kaoutar Anouar Tadlaoui |
| collection | DOAJ |
| description | Aim: The aim of this study is to investigate the prevalence of human papillomavirus (HPV) genotypes in Moroccan women diagnosed with invasive cervical cancer and to assess the association between HPV infection and some socio-demographic characteristics and clinicopathological features. Methods: In this study, 80 fresh biopsies from patients with confirmed diagnoses of cervical cancer during the study period (2020–2021) were collected. All cases were subject to HPV detection by nested PCR using MY09/11 and GP5+/6+ primers. HPV genotyping was performed by type-specific PCR targeting HPV 6, 11, 16, 18, 31, and 33. Results: The average age of patients was 54 years. Most patients were diagnosed with squamous cell carcinoma (SCC; 82.5%) at stage II (71.3%). Overall, 91.3% of cervical cancer cases were HPV-positive. HPV 16 is the most prevalent genotype, reported in 60.3% of HPV-positive cases, followed by HPV 18, 33, and 31 genotypes, identified in 20.5%, 12.3%, and 6.8%, respectively. No double infection with these genotypes was observed. Statistical analysis showed a significant correlation between HPV infection and age at menarche (P = 0.028), parity (P = 0.004), childbirth delivery (P = 0.040), and marital status (P = 0.042). Conclusions: HPV-DNA was prevalent in most examined cervical cancer tissues and HPV 16, HPV 18, HPV 33, and HPV 31 were present, at single infection, in all HPV-positive cases. These results emphasize already reported data on HPV distribution in Morocco and may contribute significantly to promoting the use of HPV DNA-based screening tests and available vaccines to limit HPV infection, viral dissemination, and cancer cervical development. |
| first_indexed | 2024-03-07T19:11:44Z |
| format | Article |
| id | doaj.art-db47d958b97b46719550aa0cae06bb75 |
| institution | Directory Open Access Journal |
| issn | 2692-3106 |
| language | English |
| last_indexed | 2024-03-07T19:11:44Z |
| publishDate | 2024-02-01 |
| publisher | Open Exploration Publishing Inc. |
| record_format | Article |
| series | Exploration of Medicine |
| spelling | doaj.art-db47d958b97b46719550aa0cae06bb752024-03-01T01:01:43ZengOpen Exploration Publishing Inc.Exploration of Medicine2692-31062024-02-0151768810.37349/emed.2024.00207Prevalence of specific human papillomavirus genotypes among Moroccan women with invasive cervical cancerKaoutar Anouar Tadlaoui0https://orcid.org/0000-0002-5850-9788Mustapha Benhessou1https://orcid.org/0000-0002-2664-1194Abdelilah Laraqui2https://orcid.org/0000-0002-1375-6021Lina Benfdil3https://orcid.org/0000-0002-0145-2522El Arbi Bouaiti4https://orcid.org/0000-0003-1568-8269Mohammed El Mzibri5https://orcid.org/0000-0002-3148-1527Moulay Mustapha Ennaji6https://orcid.org/0000-0001-5809-0270Team of Virology, Oncology, and Biotechnologies, Laboratory of Virology, Oncology, Biosciences, Environment and New Energies (LVO-BEEN), Faculty of Sciences and Techniques Mohammedia, University Hassan II of Casablanca, Mohammedia 20650, MoroccoTeam of Virology, Oncology, and Biotechnologies, Laboratory of Virology, Oncology, Biosciences, Environment and New Energies (LVO-BEEN), Faculty of Sciences and Techniques Mohammedia, University Hassan II of Casablanca, Mohammedia 20650, Morocco; Department of Gynecology and Obstetrics, Ibn Rochd University Hospital, Faculty of Medicine and Pharmacy, University Hassan II of Casablanca, Casablanca 20360, MoroccoSequencing Unit, Laboratory of Virology, Center of Virology, Infectious and Tropical Diseases, Faculty of Medicine and Pharmacy, Mohammed V Military Teaching Hospital, Mohammed V University in Rabat, Rabat 10100, MoroccoDepartment of Gynecology and Obstetrics, Ibn Rochd University Hospital, Faculty of Medicine and Pharmacy, University Hassan II of Casablanca, Casablanca 20360, MoroccoLaboratory of Biostatistics, Clinical Research and Epidemiology, Faculty of Medicine and Pharmacy, Mohammed V University in Rabat, Rabat 10100, MoroccoUnit of Biology and Medical Research, National Center of Energy, Sciences and Nuclear Techniques, Rabat 10001, MoroccoTeam of Virology, Oncology, and Biotechnologies, Laboratory of Virology, Oncology, Biosciences, Environment and New Energies (LVO-BEEN), Faculty of Sciences and Techniques Mohammedia, University Hassan II of Casablanca, Mohammedia 20650, MoroccoAim: The aim of this study is to investigate the prevalence of human papillomavirus (HPV) genotypes in Moroccan women diagnosed with invasive cervical cancer and to assess the association between HPV infection and some socio-demographic characteristics and clinicopathological features. Methods: In this study, 80 fresh biopsies from patients with confirmed diagnoses of cervical cancer during the study period (2020–2021) were collected. All cases were subject to HPV detection by nested PCR using MY09/11 and GP5+/6+ primers. HPV genotyping was performed by type-specific PCR targeting HPV 6, 11, 16, 18, 31, and 33. Results: The average age of patients was 54 years. Most patients were diagnosed with squamous cell carcinoma (SCC; 82.5%) at stage II (71.3%). Overall, 91.3% of cervical cancer cases were HPV-positive. HPV 16 is the most prevalent genotype, reported in 60.3% of HPV-positive cases, followed by HPV 18, 33, and 31 genotypes, identified in 20.5%, 12.3%, and 6.8%, respectively. No double infection with these genotypes was observed. Statistical analysis showed a significant correlation between HPV infection and age at menarche (P = 0.028), parity (P = 0.004), childbirth delivery (P = 0.040), and marital status (P = 0.042). Conclusions: HPV-DNA was prevalent in most examined cervical cancer tissues and HPV 16, HPV 18, HPV 33, and HPV 31 were present, at single infection, in all HPV-positive cases. These results emphasize already reported data on HPV distribution in Morocco and may contribute significantly to promoting the use of HPV DNA-based screening tests and available vaccines to limit HPV infection, viral dissemination, and cancer cervical development.https://www.explorationpub.com/Journals/em/Article/1001207moroccocervical canceroncogenic human papillomaviruspcrgenotyping |
| spellingShingle | Kaoutar Anouar Tadlaoui Mustapha Benhessou Abdelilah Laraqui Lina Benfdil El Arbi Bouaiti Mohammed El Mzibri Moulay Mustapha Ennaji Prevalence of specific human papillomavirus genotypes among Moroccan women with invasive cervical cancer Exploration of Medicine morocco cervical cancer oncogenic human papillomavirus pcr genotyping |
| title | Prevalence of specific human papillomavirus genotypes among Moroccan women with invasive cervical cancer |
| title_full | Prevalence of specific human papillomavirus genotypes among Moroccan women with invasive cervical cancer |
| title_fullStr | Prevalence of specific human papillomavirus genotypes among Moroccan women with invasive cervical cancer |
| title_full_unstemmed | Prevalence of specific human papillomavirus genotypes among Moroccan women with invasive cervical cancer |
| title_short | Prevalence of specific human papillomavirus genotypes among Moroccan women with invasive cervical cancer |
| title_sort | prevalence of specific human papillomavirus genotypes among moroccan women with invasive cervical cancer |
| topic | morocco cervical cancer oncogenic human papillomavirus pcr genotyping |
| url | https://www.explorationpub.com/Journals/em/Article/1001207 |
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