Microbial 2-butanol production with Lactobacillus diolivorans
Abstract Background Biobutanol has great potential as biofuel of the future. However, only a few organisms have the natural ability to produce butanol. Amongst them, Clostridium spp. are the most efficient producers. The high toxicity of biobutanol constitutes one of the bottlenecks within the biobu...
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| Format: | Article |
| Language: | English |
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BMC
2019-11-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-019-1594-5 |
| _version_ | 1828729982703108096 |
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| author | Hannes Russmayer Hans Marx Michael Sauer |
| author_facet | Hannes Russmayer Hans Marx Michael Sauer |
| author_sort | Hannes Russmayer |
| collection | DOAJ |
| description | Abstract Background Biobutanol has great potential as biofuel of the future. However, only a few organisms have the natural ability to produce butanol. Amongst them, Clostridium spp. are the most efficient producers. The high toxicity of biobutanol constitutes one of the bottlenecks within the biobutanol production process which often suffers from low final butanol concentrations and yields. Butanol tolerance is a key driver for process optimisation and, therefore, in the search for alternative butanol production hosts. Many Lactobacillus species show a remarkable tolerance to solvents and some Lactobacillus spp. are known to naturally produce 2-butanol from meso-2,3-butanediol (meso-2,3-BTD) during anaerobic sugar fermentations. Lactobacillus diolivorans showed already to be highly efficient in the production of other bulk chemicals using a simple two-step metabolic pathway. Exactly, the same pathway enables this cell factory for 2-butanol production. Results Due to the inability of L. diolivorans to produce meso-2,3-BTD, a two-step cultivation processes with Serratia marcescens has been developed. S. marcescens is a very efficient producer of meso-2,3-BTD from glucose. The process yielded a butanol concentration of 10 g/L relying on wild-type bacterial strains. A further improvement of the maximum butanol titer was achieved using an engineered L. diolivorans strain overexpressing the endogenous alcohol dehydrogenase pduQ. The two-step cultivation process based on the engineered strain led to a maximum 2-butanol titer of 13.4 g/L, which is an increase of 34%. Conclusion In this study, L. diolivorans is for the first time described as a good natural producer for 2-butanol from meso-2,3-butanediol. Through the application of a two-step cultivation process with S. marcescens, 2-butanol can be produced from glucose in a one-vessel, two-step microbial process. |
| first_indexed | 2024-04-12T14:41:05Z |
| format | Article |
| id | doaj.art-db4d379926a245f995cac2726bfdbbd0 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-04-12T14:41:05Z |
| publishDate | 2019-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-db4d379926a245f995cac2726bfdbbd02022-12-22T03:28:51ZengBMCBiotechnology for Biofuels1754-68342019-11-0112111110.1186/s13068-019-1594-5Microbial 2-butanol production with Lactobacillus diolivoransHannes Russmayer0Hans Marx1Michael Sauer2CD Laboratory for Biotechnology of GlycerolCD Laboratory for Biotechnology of GlycerolCD Laboratory for Biotechnology of GlycerolAbstract Background Biobutanol has great potential as biofuel of the future. However, only a few organisms have the natural ability to produce butanol. Amongst them, Clostridium spp. are the most efficient producers. The high toxicity of biobutanol constitutes one of the bottlenecks within the biobutanol production process which often suffers from low final butanol concentrations and yields. Butanol tolerance is a key driver for process optimisation and, therefore, in the search for alternative butanol production hosts. Many Lactobacillus species show a remarkable tolerance to solvents and some Lactobacillus spp. are known to naturally produce 2-butanol from meso-2,3-butanediol (meso-2,3-BTD) during anaerobic sugar fermentations. Lactobacillus diolivorans showed already to be highly efficient in the production of other bulk chemicals using a simple two-step metabolic pathway. Exactly, the same pathway enables this cell factory for 2-butanol production. Results Due to the inability of L. diolivorans to produce meso-2,3-BTD, a two-step cultivation processes with Serratia marcescens has been developed. S. marcescens is a very efficient producer of meso-2,3-BTD from glucose. The process yielded a butanol concentration of 10 g/L relying on wild-type bacterial strains. A further improvement of the maximum butanol titer was achieved using an engineered L. diolivorans strain overexpressing the endogenous alcohol dehydrogenase pduQ. The two-step cultivation process based on the engineered strain led to a maximum 2-butanol titer of 13.4 g/L, which is an increase of 34%. Conclusion In this study, L. diolivorans is for the first time described as a good natural producer for 2-butanol from meso-2,3-butanediol. Through the application of a two-step cultivation process with S. marcescens, 2-butanol can be produced from glucose in a one-vessel, two-step microbial process.http://link.springer.com/article/10.1186/s13068-019-1594-5Industrial microbiologyMetabolic engineering2,3-Butanediol2-ButanoneSerratia marcescens |
| spellingShingle | Hannes Russmayer Hans Marx Michael Sauer Microbial 2-butanol production with Lactobacillus diolivorans Biotechnology for Biofuels Industrial microbiology Metabolic engineering 2,3-Butanediol 2-Butanone Serratia marcescens |
| title | Microbial 2-butanol production with Lactobacillus diolivorans |
| title_full | Microbial 2-butanol production with Lactobacillus diolivorans |
| title_fullStr | Microbial 2-butanol production with Lactobacillus diolivorans |
| title_full_unstemmed | Microbial 2-butanol production with Lactobacillus diolivorans |
| title_short | Microbial 2-butanol production with Lactobacillus diolivorans |
| title_sort | microbial 2 butanol production with lactobacillus diolivorans |
| topic | Industrial microbiology Metabolic engineering 2,3-Butanediol 2-Butanone Serratia marcescens |
| url | http://link.springer.com/article/10.1186/s13068-019-1594-5 |
| work_keys_str_mv | AT hannesrussmayer microbial2butanolproductionwithlactobacillusdiolivorans AT hansmarx microbial2butanolproductionwithlactobacillusdiolivorans AT michaelsauer microbial2butanolproductionwithlactobacillusdiolivorans |