AdipoR1 and AdipoR2 maintain membrane fluidity in most human cell types and independently of adiponectin
The FA composition of phospholipids must be tightly regulated to maintain optimal cell membrane properties and compensate for a highly variable supply of dietary FAs. Previous studies have shown that AdipoR2 and its homologue PAQR-2 are important regulators of phospholipid FA composition in HEK293 c...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-05-01
|
| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520322690 |
| _version_ | 1818408740864393216 |
|---|---|
| author | Mario Ruiz Marcus Ståhlman Jan Borén Marc Pilon |
| author_facet | Mario Ruiz Marcus Ståhlman Jan Borén Marc Pilon |
| author_sort | Mario Ruiz |
| collection | DOAJ |
| description | The FA composition of phospholipids must be tightly regulated to maintain optimal cell membrane properties and compensate for a highly variable supply of dietary FAs. Previous studies have shown that AdipoR2 and its homologue PAQR-2 are important regulators of phospholipid FA composition in HEK293 cells and Caenorhabditis elegans, respectively. Here we show that both AdipoR1 and AdipoR2 are essential for sustaining desaturase expression and high levels of unsaturated FAs in membrane phospholipids of many human cell types, including primary human umbilical vein endothelial cells, and for preventing membrane rigidification in cells challenged with exogenous palmitate, a saturated FA. Three independent methods confirm the role of the AdipoRs as regulators of membrane composition and fluidity: fluorescence recovery after photobleaching, measurements of Laurdan dye generalized polarization, and mass spectrometry to determine the FA composition of phospholipids. Furthermore, we show that the AdipoRs can prevent lipotoxicity in the complete absence of adiponectin, their putative ligand. We propose that the primary cellular function of AdipoR1 and AdipoR2 is to maintain membrane fluidity in most human cell types and that adiponectin is not required for this function. |
| first_indexed | 2024-12-14T09:48:32Z |
| format | Article |
| id | doaj.art-db4da3b7658d4b8da6451a37e5f02683 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-14T09:48:32Z |
| publishDate | 2019-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-db4da3b7658d4b8da6451a37e5f026832022-12-21T23:07:35ZengElsevierJournal of Lipid Research0022-22752019-05-016059951004AdipoR1 and AdipoR2 maintain membrane fluidity in most human cell types and independently of adiponectinMario Ruiz0Marcus Ståhlman1Jan Borén2Marc Pilon3Department of Chemistry and Molecular Biology; University of Gothenburg, Gothenburg, SwedenDepartment of Molecular and Clinical Medicine/Wallenberg Laboratory Institute of Medicine, University of Gothenburg, Gothenburg, SwedenDepartment of Molecular and Clinical Medicine/Wallenberg Laboratory Institute of Medicine, University of Gothenburg, Gothenburg, SwedenTo whom correspondence should be addressed.; Department of Chemistry and Molecular Biology; University of Gothenburg, Gothenburg, Sweden; To whom correspondence should be addressed.The FA composition of phospholipids must be tightly regulated to maintain optimal cell membrane properties and compensate for a highly variable supply of dietary FAs. Previous studies have shown that AdipoR2 and its homologue PAQR-2 are important regulators of phospholipid FA composition in HEK293 cells and Caenorhabditis elegans, respectively. Here we show that both AdipoR1 and AdipoR2 are essential for sustaining desaturase expression and high levels of unsaturated FAs in membrane phospholipids of many human cell types, including primary human umbilical vein endothelial cells, and for preventing membrane rigidification in cells challenged with exogenous palmitate, a saturated FA. Three independent methods confirm the role of the AdipoRs as regulators of membrane composition and fluidity: fluorescence recovery after photobleaching, measurements of Laurdan dye generalized polarization, and mass spectrometry to determine the FA composition of phospholipids. Furthermore, we show that the AdipoRs can prevent lipotoxicity in the complete absence of adiponectin, their putative ligand. We propose that the primary cellular function of AdipoR1 and AdipoR2 is to maintain membrane fluidity in most human cell types and that adiponectin is not required for this function.http://www.sciencedirect.com/science/article/pii/S0022227520322690fatty acidsdesaturasesphospholipidsmetabolismlipotoxicityreceptors |
| spellingShingle | Mario Ruiz Marcus Ståhlman Jan Borén Marc Pilon AdipoR1 and AdipoR2 maintain membrane fluidity in most human cell types and independently of adiponectin Journal of Lipid Research fatty acids desaturases phospholipids metabolism lipotoxicity receptors |
| title | AdipoR1 and AdipoR2 maintain membrane fluidity in most human cell types and independently of adiponectin |
| title_full | AdipoR1 and AdipoR2 maintain membrane fluidity in most human cell types and independently of adiponectin |
| title_fullStr | AdipoR1 and AdipoR2 maintain membrane fluidity in most human cell types and independently of adiponectin |
| title_full_unstemmed | AdipoR1 and AdipoR2 maintain membrane fluidity in most human cell types and independently of adiponectin |
| title_short | AdipoR1 and AdipoR2 maintain membrane fluidity in most human cell types and independently of adiponectin |
| title_sort | adipor1 and adipor2 maintain membrane fluidity in most human cell types and independently of adiponectin |
| topic | fatty acids desaturases phospholipids metabolism lipotoxicity receptors |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520322690 |
| work_keys_str_mv | AT marioruiz adipor1andadipor2maintainmembranefluidityinmosthumancelltypesandindependentlyofadiponectin AT marcusstahlman adipor1andadipor2maintainmembranefluidityinmosthumancelltypesandindependentlyofadiponectin AT janboren adipor1andadipor2maintainmembranefluidityinmosthumancelltypesandindependentlyofadiponectin AT marcpilon adipor1andadipor2maintainmembranefluidityinmosthumancelltypesandindependentlyofadiponectin |